An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts

Detalhes bibliográficos
Autor(a) principal: Guimarães,Erick Vaz
Data de Publicação: 2003
Outros Autores: Carvalho,Laís de, Barbosa,Helene Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700010
Resumo: Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.
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spelling An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cystsToxoplasma gondiiamylopectin granulestissue cystsultrastructural cytochemistryUltrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.Instituto Oswaldo Cruz, Ministério da Saúde2003-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700010Memórias do Instituto Oswaldo Cruz v.98 n.7 2003reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762003000700010info:eu-repo/semantics/openAccessGuimarães,Erick VazCarvalho,Laís deBarbosa,Helene Santoseng2020-04-25T17:49:08Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:12.513Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
spellingShingle An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
Guimarães,Erick Vaz
Toxoplasma gondii
amylopectin granules
tissue cysts
ultrastructural cytochemistry
title_short An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_full An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_fullStr An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_full_unstemmed An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
title_sort An alternative technique to reveal polysaccharides in Toxoplasma gondii tissue cysts
author Guimarães,Erick Vaz
author_facet Guimarães,Erick Vaz
Carvalho,Laís de
Barbosa,Helene Santos
author_role author
author2 Carvalho,Laís de
Barbosa,Helene Santos
author2_role author
author
dc.contributor.author.fl_str_mv Guimarães,Erick Vaz
Carvalho,Laís de
Barbosa,Helene Santos
dc.subject.por.fl_str_mv Toxoplasma gondii
amylopectin granules
tissue cysts
ultrastructural cytochemistry
topic Toxoplasma gondii
amylopectin granules
tissue cysts
ultrastructural cytochemistry
dc.description.none.fl_txt_mv Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.
description Ultrathin sections of tissue cysts isolated from the brain of Toxoplasma gondii infected mice were submitted to two different methodologies derived from the periodic acid - Schiff's reagent (PAS) technique. The use of osmium tetroxide vapor as a developing agent of the aldehyde oxidation to reveal polysaccharides with periodic acid resulted in positive reaction in amylopectin granules in bradyzoites, as well as in the wall and matrix of the cysts, with excellent increment of the ultrastructural morphology. This technique can be used for study of T. gondii-host cell intracellular cycle, the differentiation tachyzoite-bradyzoite, and also for the formation of cysts into the host cells.
publishDate 2003
dc.date.none.fl_str_mv 2003-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700010
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762003000700010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762003000700010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.98 n.7 2003
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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