Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing

Detalhes bibliográficos
Autor(a) principal: Sampaio, Susana Carla Pires Sampaio de Oliveira
Data de Publicação: 2013
Outros Autores: Monteiro, Juliana Santos de Carvalho, Cangussú, Maria Cristina Teixeira, Santos, Gustavo Miranda Pires, Santos, Marcos André Vannier dos, Santos, Jean Nunes dos, Pinheiro, Antonio Luiz Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15104
Resumo: Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brasil
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spelling Sampaio, Susana Carla Pires Sampaio de OliveiraMonteiro, Juliana Santos de CarvalhoCangussú, Maria Cristina TeixeiraSantos, Gustavo Miranda PiresSantos, Marcos André Vannier dosSantos, Jean Nunes dosPinheiro, Antonio Luiz Barbosa2016-07-22T14:25:46Z2016-07-22T14:25:46Z2013SAMPAIO, S. C. P. S. O. et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers in Medical Sciences, v. 28, p. 799–806, 2013.0268-8921https://www.arca.fiocruz.br/handle/icict/1510410.1007/s10103-012-1161-9engSpringer VerlagEffect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, BrasilFederal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, BrasilFederal University of Bahia. School of Dentistry. Oral Epidemiology and Public Health. Salvador, BA, BrasilFundação Gonçalo Moniz. Laboratório de Biologia Parasitária. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Laboratório de Biologia Parasitária. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. School of Dentistry. Laboratory of Surgical Pathology. Salvador, BA, BrasilUniversity of São Paulo. National Institute of Optics and Photonics. Physics Institute of São Carlos. São Paulo, SP, BrasilIron deficiency impairs the formation of hemoglobin, red blood cells, as well the transport of oxygen. The wound healing process involves numerous functions, many of which are dependent on the presence of oxygen. Laser has been shown to improve angiogenesis, increases blood supply, cell proliferation and function. We aimed to study the effect of λ660 nm laser and λ700 nm light-emitting diode (LED) on fibroblastic proliferation on cutaneous wounds on iron-deficient rodents. Induction of iron anemia was carried out by feeding 105 newborn rats with a special iron-free diet. A 1 × 1 cm wound was created on the dorsum of each animal that were randomly distributed into seven groups: I, control anemic; II, anemic no treatment; III, anemic+L; IV, anemic+LED; V, healthy no treatment; VI, healthy+laser; VII, healthy+LED (n=15 each). Phototherapy was carried out using either a diode laser (λ660 nm, 40 mW, 10 J/cm(2)) or a prototype LED device (λ700 ± 20 nm, 15 mW, 10 J/cm(2)). Treatment started immediately after surgery and was repeated at 48-h interval during 7, 14, and 21 days. After animal death, specimens were taken, routinely processed, cut, stained with hematoxylin-eosin, and underwent histological analysis and fibroblast counting. Significant difference between healthy and anemic subjects on regards the number of fibroblast between treatments was seen (p<0.008, p<0.001). On healthy animals, significant higher count was seen when laser was used (p<0.008). Anemic subjects irradiated with LED showed significantly higher count (p<0.001). It is concluded that the use of LED light caused a significant positive biomodulation of fibroblastic proliferation on anemic animals and laser was more effective on increasing proliferation on non-anemics.AnemiaPhototherapyWound healingCell ProliferationFibroblastsAnimalsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15104/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALOliveira SCPS Effect of laser....pdfOliveira SCPS Effect of laser....pdfapplication/pdf596479https://www.arca.fiocruz.br/bitstream/icict/15104/2/Oliveira%20SCPS%20%20Effect%20of%20laser....pdf3f816d2fe808e6e420b7fe1e50f1b436MD52TEXTOliveira SCPS Effect of laser....pdf.txtOliveira SCPS Effect of laser....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
title Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
spellingShingle Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
Sampaio, Susana Carla Pires Sampaio de Oliveira
Anemia
Phototherapy
Wound healing
Cell Proliferation
Fibroblasts
Animals
title_short Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
title_full Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
title_fullStr Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
title_full_unstemmed Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
title_sort Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing
author Sampaio, Susana Carla Pires Sampaio de Oliveira
author_facet Sampaio, Susana Carla Pires Sampaio de Oliveira
Monteiro, Juliana Santos de Carvalho
Cangussú, Maria Cristina Teixeira
Santos, Gustavo Miranda Pires
Santos, Marcos André Vannier dos
Santos, Jean Nunes dos
Pinheiro, Antonio Luiz Barbosa
author_role author
author2 Monteiro, Juliana Santos de Carvalho
Cangussú, Maria Cristina Teixeira
Santos, Gustavo Miranda Pires
Santos, Marcos André Vannier dos
Santos, Jean Nunes dos
Pinheiro, Antonio Luiz Barbosa
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sampaio, Susana Carla Pires Sampaio de Oliveira
Monteiro, Juliana Santos de Carvalho
Cangussú, Maria Cristina Teixeira
Santos, Gustavo Miranda Pires
Santos, Marcos André Vannier dos
Santos, Jean Nunes dos
Pinheiro, Antonio Luiz Barbosa
dc.subject.en.pt_BR.fl_str_mv Anemia
Phototherapy
Wound healing
Cell Proliferation
Fibroblasts
Animals
topic Anemia
Phototherapy
Wound healing
Cell Proliferation
Fibroblasts
Animals
description Federal University of Bahia. School of Dentistry. Center of Biophotonics. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-07-22T14:25:46Z
dc.date.available.fl_str_mv 2016-07-22T14:25:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SAMPAIO, S. C. P. S. O. et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers in Medical Sciences, v. 28, p. 799–806, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15104
dc.identifier.issn.pt_BR.fl_str_mv 0268-8921
dc.identifier.doi.none.fl_str_mv 10.1007/s10103-012-1161-9
identifier_str_mv SAMPAIO, S. C. P. S. O. et al. Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers in Medical Sciences, v. 28, p. 799–806, 2013.
0268-8921
10.1007/s10103-012-1161-9
url https://www.arca.fiocruz.br/handle/icict/15104
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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