Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/42578 |
Resumo: | Acesso aberto em 06/08/2020 - https://www.sciencedirect.com/science/article/pii/S2052297520300743 |
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Conceição Neto, O. C.Costa, B. S. daPontes, L. S.Santos, I. C. O.Silveira, M. C.Moura, J. R. CordeiroPereira, N. F.Teixeira, C. B. TavaresPicão, R. C.Souza, C. M. Rocha deCarvalho-Assef, A. P. D´A.2020-08-06T18:52:36Z2020-08-06T18:52:36Z2020CONCEIÇÃO NETO, O. C. et al. Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium. New Microbe and New Infect., v. 36, 100722, 7p, 2020.2052-2975https://www.arca.fiocruz.br/handle/icict/4257810.1016/j.nmni.2020.100722Acesso aberto em 06/08/2020 - https://www.sciencedirect.com/science/article/pii/S2052297520300743Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Investigação em Microbiologia Médica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil.Polymyxins are important therapeutic options for treating infections, mainly those caused by carbapenem-resistant Klebsiella pneumoniae. Specific chemical characteristics of polymyxins make it difficult to perform antimicrobial susceptibility testing, especially within the clinical laboratory. Here we aimed to evaluate the performance of three phenotypic methods: Rapid NP Polymyxin Test, ColiSpot test and the SuperPolymyxin medium. To accomplish this, 170 non-duplicate clinical K. pneumoniae isolates were analysed (123 colistin-resistant and 47 susceptible). The sensitivity and specificity obtained for Rapid Polymyxin NP Test, Colispot and SuperPolymyxin medium were, respectively, 90% and 94%, 74% and 100%, and 82% and 85%. Very major errors occurred more frequently in low-level colistin-resistant isolates (MICs 4 and 8 μg/mL). Rapid Polymyxin NP proved to be a method capable of identifying colistin-resistant strains in acceptable categorical agreement. However, major errors and very major errors of this method were considered unacceptable for colistinresistance screening. Although the Colispot test is promising and easy to perform and interpret, the results did not reproduce well in the isolates tested. The colistin-containing selective medium (SuperPolymyxin) showed limitations, including quantification of mucoid colonies and poor stability. Nevertheless, Colispot and SuperPolymyxin medium methods did not present acceptable sensitivity, specificity and categorical agreement. It is essential to use analytical tools that faithfully reproduce bacterial resistance in vitro, especially in last-line drugs, such as polymyxins, when misinterpretation of a test can result in therapeutic ineffectiveness.engElsevierResistência a antibióticosIsolados clínicosKlebsiella pneumoniaeDetecção fenotípicaResistência à polimíxinaAntibiotic resistanceClinical isolatesKlebsiella pneumoniaePhenotypic detectionPolymyxin resistanceDifficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin mediuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| title |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| spellingShingle |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium Conceição Neto, O. C. Resistência a antibióticos Isolados clínicos Klebsiella pneumoniae Detecção fenotípica Resistência à polimíxina Antibiotic resistance Clinical isolates Klebsiella pneumoniae Phenotypic detection Polymyxin resistance |
| title_short |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| title_full |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| title_fullStr |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| title_full_unstemmed |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| title_sort |
Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium |
| author |
Conceição Neto, O. C. |
| author_facet |
Conceição Neto, O. C. Costa, B. S. da Pontes, L. S. Santos, I. C. O. Silveira, M. C. Moura, J. R. Cordeiro Pereira, N. F. Teixeira, C. B. Tavares Picão, R. C. Souza, C. M. Rocha de Carvalho-Assef, A. P. D´A. |
| author_role |
author |
| author2 |
Costa, B. S. da Pontes, L. S. Santos, I. C. O. Silveira, M. C. Moura, J. R. Cordeiro Pereira, N. F. Teixeira, C. B. Tavares Picão, R. C. Souza, C. M. Rocha de Carvalho-Assef, A. P. D´A. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Conceição Neto, O. C. Costa, B. S. da Pontes, L. S. Santos, I. C. O. Silveira, M. C. Moura, J. R. Cordeiro Pereira, N. F. Teixeira, C. B. Tavares Picão, R. C. Souza, C. M. Rocha de Carvalho-Assef, A. P. D´A. |
| dc.subject.other.pt_BR.fl_str_mv |
Resistência a antibióticos Isolados clínicos Klebsiella pneumoniae Detecção fenotípica Resistência à polimíxina |
| topic |
Resistência a antibióticos Isolados clínicos Klebsiella pneumoniae Detecção fenotípica Resistência à polimíxina Antibiotic resistance Clinical isolates Klebsiella pneumoniae Phenotypic detection Polymyxin resistance |
| dc.subject.en.pt_BR.fl_str_mv |
Antibiotic resistance Clinical isolates Klebsiella pneumoniae Phenotypic detection Polymyxin resistance |
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Acesso aberto em 06/08/2020 - https://www.sciencedirect.com/science/article/pii/S2052297520300743 |
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2020 |
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2020-08-06T18:52:36Z |
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2020-08-06T18:52:36Z |
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2020 |
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CONCEIÇÃO NETO, O. C. et al. Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium. New Microbe and New Infect., v. 36, 100722, 7p, 2020. |
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https://www.arca.fiocruz.br/handle/icict/42578 |
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2052-2975 |
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10.1016/j.nmni.2020.100722 |
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CONCEIÇÃO NETO, O. C. et al. Difficulty in detecting low levels of polymyxin resistance in clinical Klebsiella pneumoniae isolates: evaluation of Rapid Polymyxin NP test, Colispot Test and SuperPolymyxin medium. New Microbe and New Infect., v. 36, 100722, 7p, 2020. 2052-2975 10.1016/j.nmni.2020.100722 |
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Elsevier |
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Elsevier |
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