Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals

Detalhes bibliográficos
Autor(a) principal: Fujiwara, Ricardo Toshio
Data de Publicação: 2009
Outros Autores: Cançado, Guilherme Grossi Lopes, Freitas, Paula de Albuquerque, Santiago, Helton da Costa, Massara, Cristiano Lara, Carvalho, Omar dos Santos, Oliveira, Rodrigo Corrêa, Geiger, Stefan Michael, Bethony, Jeffrey Michael
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58845
Resumo: Laboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation., Belo Horizonte, MG, Brazil/Department of Microbiology, Immunology and Tropical Medicine. The George Washington University. Washington, D.C., United States of America/Department of Parasitology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil
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spelling Fujiwara, Ricardo ToshioCançado, Guilherme Grossi LopesFreitas, Paula de AlbuquerqueSantiago, Helton da CostaMassara, Cristiano LaraCarvalho, Omar dos SantosOliveira, Rodrigo CorrêaGeiger, Stefan MichaelBethony, Jeffrey Michael2023-05-31T19:13:37Z2023-05-31T19:13:37Z2009FUJIWARA, Ricardo Toshio et al. Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals. PLoS Negl Trop Dis., v. 3, n. 3, e399, 2009. doi.org/10.1371/journal.pntd.00003991935-2735https://www.arca.fiocruz.br/handle/icict/5884510.1371/journal.pntd.0000399engPublic Library of ScienceNecator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation., Belo Horizonte, MG, Brazil/Department of Microbiology, Immunology and Tropical Medicine. The George Washington University. Washington, D.C., United States of America/Department of Parasitology. Federal University of Minas Gerais. Belo Horizonte, MG, BrazilLaboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilLaboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation., Belo Horizonte, MG, BrazilDepartment of Microbiology, Immunology and Tropical Medicine, The George Washington University, Washington, D.C., United States of AmericaLaboratory of Helminthology and Medical Malacology. Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilLaboratory of Helminthology and Medical Malacology. Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilLaboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation., Belo Horizonte, MG, BrazilLaboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation., Belo Horizonte, MG, BrazilDepartment of Microbiology, Immunology and Tropical Medicine. The George Washington University. Washington, D.C., United States of AmericaBackground: Hookworms survive for several years (5 to 7 years) in the host lumen, inducing a robust but largely ineffective immune response. Among the most striking aspects of the immune response to hookworm (as with many other helminths) is the ablation of parasite-specific T cell proliferative response (hyporesponsiveness). While the role of the adaptive immune response in human helminth infection has been well investigated, the role of the innate immune responses (e. g., dendritic cells and eosinophils) has received less attention and remains to be clearly elucidated. Methodology/Principal Findings: We report on the differentiation/maturation of host dendritic cells in vitro and the eosinophil activation/function associated with human hookworm infection. Mature DCs (mDCs) from Necator americanus (Necator)-infected individuals showed an impaired differentiation process compared to the mDCs of non-infected individuals, as evidenced by the differential expression of CD11c and CD14. These same hookworm-infected individuals also presented significantly down-regulated expression of CD86, CD1a, HLA-ABC, and HLA-DR. The lower expression of costimulatory and antigen presentation molecules by hookworm-infected-derived mDCs was further evidenced by their reduced ability to induce cell proliferation. We also showed that this alternative DC differentiation is partially induced by excreted-secreted hookworm products. Conversely, eosinophils from the same individuals showed a highly activated status, with an upregulation of major cell surface markers. Antigen-pulsed eosinophils from N. americanus-infected individuals induced significant cell proliferation of autologous PBMCs, when compared to non-infected individuals. Conclusion: Chronic N. americanus infection alters the host's innate immune response, resulting in a possible modulation of the maturation process of DCs, a functional change that may diminish their ability for antigen presentation and thus contribute to the ablation of the parasite-specific T cell proliferative response. Interestingly, a concomitant upregulation of the major cell surface markers of eosinophils was observed in hookworm-infected individuals, indicative of antigen-specific immune responses, especially antigen presentation. We showed that in addition to the postulated role of the eosinophils as effector cells against helminth infection, activated cells may also be recruited to sites of inflammation and contribute to the immune response acting as antigen presenting cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALpntd.0000399 1..10.pdfpntd.0000399 1..10.pdfapplication/pdf433572https://www.arca.fiocruz.br/bitstream/icict/58845/2/pntd.0000399%201..10.pdf918b8915e04d0d8641727a2303fbeeeaMD52LICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/58845/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51icict/588452023-05-31 16:13:38.0oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-05-31T19:13:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
title Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
spellingShingle Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
Fujiwara, Ricardo Toshio
title_short Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
title_full Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
title_fullStr Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
title_full_unstemmed Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
title_sort Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals
author Fujiwara, Ricardo Toshio
author_facet Fujiwara, Ricardo Toshio
Cançado, Guilherme Grossi Lopes
Freitas, Paula de Albuquerque
Santiago, Helton da Costa
Massara, Cristiano Lara
Carvalho, Omar dos Santos
Oliveira, Rodrigo Corrêa
Geiger, Stefan Michael
Bethony, Jeffrey Michael
author_role author
author2 Cançado, Guilherme Grossi Lopes
Freitas, Paula de Albuquerque
Santiago, Helton da Costa
Massara, Cristiano Lara
Carvalho, Omar dos Santos
Oliveira, Rodrigo Corrêa
Geiger, Stefan Michael
Bethony, Jeffrey Michael
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fujiwara, Ricardo Toshio
Cançado, Guilherme Grossi Lopes
Freitas, Paula de Albuquerque
Santiago, Helton da Costa
Massara, Cristiano Lara
Carvalho, Omar dos Santos
Oliveira, Rodrigo Corrêa
Geiger, Stefan Michael
Bethony, Jeffrey Michael
description Laboratory of Cellular and Molecular Immunology. Instituto René Rachou. Oswaldo Cruz Foundation., Belo Horizonte, MG, Brazil/Department of Microbiology, Immunology and Tropical Medicine. The George Washington University. Washington, D.C., United States of America/Department of Parasitology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2023-05-31T19:13:37Z
dc.date.available.fl_str_mv 2023-05-31T19:13:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv FUJIWARA, Ricardo Toshio et al. Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals. PLoS Negl Trop Dis., v. 3, n. 3, e399, 2009. doi.org/10.1371/journal.pntd.0000399
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58845
dc.identifier.issn.en_US.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0000399
identifier_str_mv FUJIWARA, Ricardo Toshio et al. Necator americanus Infection: A Possible Cause of Altered Dendritic Cell Differentiation and Eosinophil Profile in Chronically Infected Individuals. PLoS Negl Trop Dis., v. 3, n. 3, e399, 2009. doi.org/10.1371/journal.pntd.0000399
1935-2735
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