A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.

Detalhes bibliográficos
Autor(a) principal: Probst, Christian M.
Data de Publicação: 2012
Outros Autores: Silva, Rodrigo de Araújo, Menezes, Juliana Perrone Bezerra de, Almeida, Taís Fontoura de, Gomes, Ivana Nunes, Dallabona, Andréia C., Ozaqui, L. S., Buck, Gregory A., Pavoni, Daniela P., Krieger, Marco Aurélio, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7727
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, Brasil
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spelling Probst, Christian M.Silva, Rodrigo de AraújoMenezes, Juliana Perrone Bezerra deAlmeida, Taís Fontoura deGomes, Ivana NunesDallabona, Andréia C.Ozaqui, L. S.Buck, Gregory A.Pavoni, Daniela P.Krieger, Marco AurélioVeras, Patrícia Sampaio Tavares2014-05-29T19:54:51Z2014-05-29T19:54:51Z2012PROBST, C. M. et al. A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection. BMC Microbiology, v. 12, p. 22, 2012.1471-2180https://www.arca.fiocruz.br/handle/icict/7727engBioMed CentralA comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, BrasilCenter for the Study of Biological Complexity. Virginia Commonwealth University. Richmond, VA, USACenter for the Study of Biological Complexity. Virginia Commonwealth University. Richmond, VA, USAFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, BrasilBACKGROUND: The experimental murine model of leishmaniasis has been widely used to characterize the immune response against Leishmania. CBA mice develop severe lesions, while C57BL/6 present small chronic lesions under L. amazonensis infection. Employing a transcriptomic approach combined with biological network analysis, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite. RESULTS: The genes expressed by C57BL/6 and CBA macrophages, before and after infection, differ greatly, both with respect to absolute number as well as cell function. Uninfected C57BL/6 macrophages express genes involved in the deactivation pathway of macrophages at lower levels, while genes related to the activation of the host immune inflammatory response, including apoptosis and phagocytosis, have elevated expression levels. Several genes that participate in the apoptosis process were also observed to be up-regulated in C57BL/6 macrophages infected with L. amazonensis, which is very likely related to the capacity of these cells to control parasite infection. By contrast, genes involved in lipid metabolism were found to be up-regulated in CBA macrophages in response to infection, which supports the notion that L. amazonensis probably modulates parasitophorous vacuoles in order to survive and multiply in host cells. CONCLUSION: The transcriptomic profiles of C57BL/6 macrophages, before and after infection, were shown to be involved in the macrophage pathway of activation, which may aid in the control of L. amazonensis infection, in contrast to the profiles of CBA cells.Perfilação da Expressão GênicaInterações Hospedeiro-PatógenoLeishmania mexicana/imunologiaLeishmania mexicana/patogenicidadeMacrófagos/imunologiaMacrófagos/parasitologiaAnimaisFemininoCélulas CultivadasMasculinoCamundongos Endogâmicos C57BLCamundongosAnálise em MicrossériesReação em Cadeia da Polimerase em Tempo Realinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALProbst CM A comparison of two....pdfProbst CM A comparison of two....pdfapplication/pdf2117744https://www.arca.fiocruz.br/bitstream/icict/7727/1/Probst%20CM%20A%20comparison%20of%20two....pdf1079177b1ef874ee88a97648be921d86MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7727/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTProbst CM A comparison of two....pdf.txtProbst CM A comparison of two....pdf.txtExtracted texttext/plain61087https://www.arca.fiocruz.br/bitstream/icict/7727/5/Probst%20CM%20A%20comparison%20of%20two....pdf.txt9dcb0e7258e9f546a6e5de353e36f4f3MD55THUMBNAILProbst CM A comparison of two....pdf.jpgProbst CM A comparison of two....pdf.jpgGenerated Thumbnailimage/jpeg2151https://www.arca.fiocruz.br/bitstream/icict/7727/4/Probst%20CM%20A%20comparison%20of%20two....pdf.jpga8260bc56746b0ce9d9186a2020e1110MD54icict/77272023-03-15 14:33:16.88oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
title A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
spellingShingle A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
Probst, Christian M.
Perfilação da Expressão Gênica
Interações Hospedeiro-Patógeno
Leishmania mexicana/imunologia
Leishmania mexicana/patogenicidade
Macrófagos/imunologia
Macrófagos/parasitologia
Animais
Feminino
Células Cultivadas
Masculino
Camundongos Endogâmicos C57BL
Camundongos
Análise em Microsséries
Reação em Cadeia da Polimerase em Tempo Real
title_short A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
title_full A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
title_fullStr A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
title_full_unstemmed A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
title_sort A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection.
author Probst, Christian M.
author_facet Probst, Christian M.
Silva, Rodrigo de Araújo
Menezes, Juliana Perrone Bezerra de
Almeida, Taís Fontoura de
Gomes, Ivana Nunes
Dallabona, Andréia C.
Ozaqui, L. S.
Buck, Gregory A.
Pavoni, Daniela P.
Krieger, Marco Aurélio
Veras, Patrícia Sampaio Tavares
author_role author
author2 Silva, Rodrigo de Araújo
Menezes, Juliana Perrone Bezerra de
Almeida, Taís Fontoura de
Gomes, Ivana Nunes
Dallabona, Andréia C.
Ozaqui, L. S.
Buck, Gregory A.
Pavoni, Daniela P.
Krieger, Marco Aurélio
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Probst, Christian M.
Silva, Rodrigo de Araújo
Menezes, Juliana Perrone Bezerra de
Almeida, Taís Fontoura de
Gomes, Ivana Nunes
Dallabona, Andréia C.
Ozaqui, L. S.
Buck, Gregory A.
Pavoni, Daniela P.
Krieger, Marco Aurélio
Veras, Patrícia Sampaio Tavares
dc.subject.decs.pt_BR.fl_str_mv Perfilação da Expressão Gênica
Interações Hospedeiro-Patógeno
Leishmania mexicana/imunologia
Leishmania mexicana/patogenicidade
Macrófagos/imunologia
Macrófagos/parasitologia
Animais
Feminino
Células Cultivadas
Masculino
Camundongos Endogâmicos C57BL
Camundongos
Análise em Microsséries
Reação em Cadeia da Polimerase em Tempo Real
topic Perfilação da Expressão Gênica
Interações Hospedeiro-Patógeno
Leishmania mexicana/imunologia
Leishmania mexicana/patogenicidade
Macrófagos/imunologia
Macrófagos/parasitologia
Animais
Feminino
Células Cultivadas
Masculino
Camundongos Endogâmicos C57BL
Camundongos
Análise em Microsséries
Reação em Cadeia da Polimerase em Tempo Real
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Paraná, SC, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-05-29T19:54:51Z
dc.date.available.fl_str_mv 2014-05-29T19:54:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PROBST, C. M. et al. A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection. BMC Microbiology, v. 12, p. 22, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7727
dc.identifier.issn.none.fl_str_mv 1471-2180
identifier_str_mv PROBST, C. M. et al. A comparison of two distinct murine macrophage gene expression profiles in response to Leishmania amazonensis infection. BMC Microbiology, v. 12, p. 22, 2012.
1471-2180
url https://www.arca.fiocruz.br/handle/icict/7727
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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