Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.

Detalhes bibliográficos
Autor(a) principal: Neves, Neuza Maria Alcântara
Data de Publicação: 1995
Outros Autores: Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8334
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, Brasil
id CRUZ_257ab0e73aafe8ca7c477fbc8b1c689f
oai_identifier_str oai:www.arca.fiocruz.br:icict/8334
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Neves, Neuza Maria AlcântaraPontes-de-Carvalho, Lain Carlos2014-09-09T16:56:31Z2014-09-09T16:56:31Z1995ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.0932-0113https://www.arca.fiocruz.br/handle/icict/8334engSpringer VerlagCirculating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, BrasilParasite-derived trans-sialidase (TS) activity was demonstrated in the serum and blood of Trypanosoma cruzi-infected mice. Serum TS activity levels correlated well with parasitemia in BALB/c and Swiss mice during the initial stages of the infection. However, in later stages the TS activity levels decreased despite increasing parasitemia. This coincided with the appearance of circulating TS antibodies. On the other hand, there was always a good correlation between TS activity and parasitemia in athymic nude mice. Sera from mice with high parasitemia and low TS activity inhibited TS activity in vitro. The inhibition was also observed with purified serum IgG, and it was absorbed by staphylococcal protein A, indicating that it was caused by anti-TS IgG antibodies. These antibodies inhibited the enzymatic activity of insolubilized TS, indicating that they act by interfering with the catalytic site rather than by aggregating the enzyme. The presence of inhibitory antibodies, however, did not prevent the progression of parasitemia in BALB/c mice.Anticorpos Antiprotozoários/sangueDoença de Chagas /sangueGlicoproteínas /sangueNeuraminidase /sangueTrypanosoma cruzi /enzimologiaAnimaisWestern BlottingDoença de Chagas /enzimologiaEletroforese em Gel de PoliacrilamidaFemininoGlicoproteínas /antagonistas & inibidoresImunoglobulina G /isolamento & purificaçãoCamundongosCamundongos Endogâmicos BALB CNeuraminidase /antagonistas & inibidoresParasitemia /enzimologiaFatores de TempoTrypanosoma cruzi /imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8334/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALAlcantara N NM Circulating....pdfAlcantara N NM Circulating....pdfapplication/pdf764426https://www.arca.fiocruz.br/bitstream/icict/8334/2/Alcantara%20N%20NM%20%20Circulating....pdf5944d5474da91486002043b53a0614d4MD52TEXTAlcantara N NM Circulating....pdf.txtAlcantara N NM Circulating....pdf.txtExtracted texttext/plain29135https://www.arca.fiocruz.br/bitstream/icict/8334/3/Alcantara%20N%20NM%20%20Circulating....pdf.txt6ef4c645c67a864701342d71c7398d8cMD53icict/83342023-03-15 14:33:07.992oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
spellingShingle Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
Neves, Neuza Maria Alcântara
Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Animais
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Feminino
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
title_short Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_full Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_fullStr Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_full_unstemmed Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_sort Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
author Neves, Neuza Maria Alcântara
author_facet Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Pontes-de-Carvalho, Lain Carlos
author2_role author
dc.contributor.author.fl_str_mv Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Animais
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Feminino
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
topic Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Animais
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Feminino
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, Brasil
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2014-09-09T16:56:31Z
dc.date.available.fl_str_mv 2014-09-09T16:56:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8334
dc.identifier.issn.none.fl_str_mv 0932-0113
identifier_str_mv ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.
0932-0113
url https://www.arca.fiocruz.br/handle/icict/8334
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/8334/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/8334/2/Alcantara%20N%20NM%20%20Circulating....pdf
https://www.arca.fiocruz.br/bitstream/icict/8334/3/Alcantara%20N%20NM%20%20Circulating....pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
5944d5474da91486002043b53a0614d4
6ef4c645c67a864701342d71c7398d8c
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798325011796197376

Registros relacionados