Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.

Detalhes bibliográficos
Autor(a) principal: Neves, Neuza Maria Alcântara
Data de Publicação: 1995
Outros Autores: Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8334
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, Brasil
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spelling Neves, Neuza Maria AlcântaraPontes-de-Carvalho, Lain Carlos2014-09-09T16:56:31Z2014-09-09T16:56:31Z1995ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.0932-0113https://www.arca.fiocruz.br/handle/icict/8334engSpringer VerlagCirculating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Púbica. Salvador, BA, BrasilParasite-derived trans-sialidase (TS) activity was demonstrated in the serum and blood of Trypanosoma cruzi-infected mice. Serum TS activity levels correlated well with parasitemia in BALB/c and Swiss mice during the initial stages of the infection. However, in later stages the TS activity levels decreased despite increasing parasitemia. This coincided with the appearance of circulating TS antibodies. On the other hand, there was always a good correlation between TS activity and parasitemia in athymic nude mice. Sera from mice with high parasitemia and low TS activity inhibited TS activity in vitro. The inhibition was also observed with purified serum IgG, and it was absorbed by staphylococcal protein A, indicating that it was caused by anti-TS IgG antibodies. These antibodies inhibited the enzymatic activity of insolubilized TS, indicating that they act by interfering with the catalytic site rather than by aggregating the enzyme. The presence of inhibitory antibodies, however, did not prevent the progression of parasitemia in BALB/c mice.Anticorpos Antiprotozoários/sangueDoença de Chagas /sangueGlicoproteínas /sangueNeuraminidase /sangueTrypanosoma cruzi /enzimologiaAnimaisWestern BlottingDoença de Chagas /enzimologiaEletroforese em Gel de PoliacrilamidaFemininoGlicoproteínas /antagonistas & inibidoresImunoglobulina G /isolamento & purificaçãoCamundongosCamundongos Endogâmicos BALB CNeuraminidase /antagonistas & inibidoresParasitemia /enzimologiaFatores de TempoTrypanosoma cruzi /imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8334/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALAlcantara N NM Circulating....pdfAlcantara N NM Circulating....pdfapplication/pdf764426https://www.arca.fiocruz.br/bitstream/icict/8334/2/Alcantara%20N%20NM%20%20Circulating....pdf5944d5474da91486002043b53a0614d4MD52TEXTAlcantara N NM Circulating....pdf.txtAlcantara N NM Circulating....pdf.txtExtracted texttext/plain29135https://www.arca.fiocruz.br/bitstream/icict/8334/3/Alcantara%20N%20NM%20%20Circulating....pdf.txt6ef4c645c67a864701342d71c7398d8cMD53icict/83342023-03-15 14:33:07.992oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
spellingShingle Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
Neves, Neuza Maria Alcântara
Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Animais
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Feminino
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
title_short Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_full Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_fullStr Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_full_unstemmed Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
title_sort Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice.
author Neves, Neuza Maria Alcântara
author_facet Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Pontes-de-Carvalho, Lain Carlos
author2_role author
dc.contributor.author.fl_str_mv Neves, Neuza Maria Alcântara
Pontes-de-Carvalho, Lain Carlos
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Animais
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Feminino
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
topic Anticorpos Antiprotozoários/sangue
Doença de Chagas /sangue
Glicoproteínas /sangue
Neuraminidase /sangue
Trypanosoma cruzi /enzimologia
Animais
Western Blotting
Doença de Chagas /enzimologia
Eletroforese em Gel de Poliacrilamida
Feminino
Glicoproteínas /antagonistas & inibidores
Imunoglobulina G /isolamento & purificação
Camundongos
Camundongos Endogâmicos BALB C
Neuraminidase /antagonistas & inibidores
Parasitemia /enzimologia
Fatores de Tempo
Trypanosoma cruzi /imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laborat6rio de Imunologia Molecular e Celular. Salvador, BA, Brasil
publishDate 1995
dc.date.issued.fl_str_mv 1995
dc.date.accessioned.fl_str_mv 2014-09-09T16:56:31Z
dc.date.available.fl_str_mv 2014-09-09T16:56:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8334
dc.identifier.issn.none.fl_str_mv 0932-0113
identifier_str_mv ALCÂNTARA-NEVES, N. M.; PONTES-DE-CARVALHO, L. C. Circulating trans-sialidase activity and trans-sialidase-inhibiting antibodies in Trypanosoma cruzi-infected mice. Parasitology Research, v. 81, n. 7, p. 560-564, 1995.
0932-0113
url https://www.arca.fiocruz.br/handle/icict/8334
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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