Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs

Detalhes bibliográficos
Autor(a) principal: Faria, Angélica Rosa
Data de Publicação: 2015
Outros Autores: Veloso, Luciano de Castro, Vital, Wendel Coura, Reis, Alexandre Barbosa, Damasceno, Leonardo Miranda, Gazzinelli, Ricardo Tostes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12609
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
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spelling Faria, Angélica RosaVeloso, Luciano de CastroVital, Wendel CouraReis, Alexandre BarbosaDamasceno, Leonardo MirandaGazzinelli, Ricardo Tostes2016-01-25T17:58:00Z2016-01-25T17:58:00Z2015FARIA, Angélica Rosa et al. Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs. PLoS Negl Trop Dis., vol. 9, n. 1, p. e3429, 20151935-2735https://www.arca.fiocruz.br/handle/icict/1260910.1371/journal.pntd.0003429engPublic Library of ScienceNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisa em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisa em Ciências Biológicas. Ouro Preto, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/University of Massachusetts Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of AmericaUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilBACKGROUND: Visceral leishmaniasis is the most severe form of leishmaniasis. Worldwide, approximately 20% of zoonotic human visceral leishmaniasis is caused by Leishmania infantum, also known as Leishmania chagasi in Latin America. Current diagnostic methods are not accurate enough to identify Leishmania-infected animals and may compromise the effectiveness of disease control. Therefore, we aimed to produce and test two recombinant multiepitope proteins as a means to improve and increase accuracy in the diagnosis of canine visceral leishmaniasis (CVL). METHODOLOGY/PRINCIPAL FINDINGS: Ten antigenic peptides were identified by CVL ELISA in previous work. In the current proposal, the coding sequences of these ten peptides were assembled into a synthetic gene. Furthermore, other twenty peptides were selected from work by our group where good B and T cell epitopes were mapped. The coding sequences of these peptides were also assembled into a synthetic gene. Both genes have been cloned and expressed in Escherichia coli, producing two multiepitope recombinant proteins, PQ10 and PQ20. These antigens have been used in CVL ELISA and were able to identify asymptomatic dogs (80%) more effectively than EIE-LVC kit, produced by Bio-Manguinhos (0%) and DPP kit (10%). Moreover, our recombinant proteins presented an early detection (before PCR) of infected dogs, with positivities ranging from 23% to 65%, depending on the phase of infection in which sera were acquired. CONCLUSIONS/SIGNIFICANCE: Our study shows that ELISA using the multiepitope proteins PQ10 and PQ20 has great potential in early CVL diagnosis. The use of these proteins in other methodologies, such as immunochromatographic tests, could be beneficial mainly for the detection of asymptomatic dogs.leishmaniasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12609/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdfNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdfapplication/pdf5117566https://www.arca.fiocruz.br/bitstream/icict/12609/2/Novel%20Recombinant%20Multiepitope%20Proteins%20for%20the%20Diagnosis%20of%20Asymptomatic%20Leishmania%20infantum-Infected%20Dogs.pdf3071ed2c6334cadac72f182d860a0b95MD52TEXTNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdf.txtNovel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs.pdf.txtExtracted texttext/plain54https://www.arca.fiocruz.br/bitstream/icict/12609/3/Novel%20Recombinant%20Multiepitope%20Proteins%20for%20the%20Diagnosis%20of%20Asymptomatic%20Leishmania%20infantum-Infected%20Dogs.pdf.txt32ceaf957275278a8f3f2b27bb2efbefMD53icict/126092019-06-19 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dc.title.pt_BR.fl_str_mv Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
title Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
spellingShingle Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
Faria, Angélica Rosa
leishmaniasis
title_short Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
title_full Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
title_fullStr Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
title_full_unstemmed Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
title_sort Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs
author Faria, Angélica Rosa
author_facet Faria, Angélica Rosa
Veloso, Luciano de Castro
Vital, Wendel Coura
Reis, Alexandre Barbosa
Damasceno, Leonardo Miranda
Gazzinelli, Ricardo Tostes
author_role author
author2 Veloso, Luciano de Castro
Vital, Wendel Coura
Reis, Alexandre Barbosa
Damasceno, Leonardo Miranda
Gazzinelli, Ricardo Tostes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Faria, Angélica Rosa
Veloso, Luciano de Castro
Vital, Wendel Coura
Reis, Alexandre Barbosa
Damasceno, Leonardo Miranda
Gazzinelli, Ricardo Tostes
dc.subject.en.pt_BR.fl_str_mv leishmaniasis
topic leishmaniasis
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-25T17:58:00Z
dc.date.available.fl_str_mv 2016-01-25T17:58:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Angélica Rosa et al. Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs. PLoS Negl Trop Dis., vol. 9, n. 1, p. e3429, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12609
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0003429
identifier_str_mv FARIA, Angélica Rosa et al. Novel Recombinant Multiepitope Proteins for the Diagnosis of Asymptomatic Leishmania infantum-Infected Dogs. PLoS Negl Trop Dis., vol. 9, n. 1, p. e3429, 2015
1935-2735
10.1371/journal.pntd.0003429
url https://www.arca.fiocruz.br/handle/icict/12609
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/12609/1/license.txt
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