Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study

Detalhes bibliográficos
Autor(a) principal: Van Weyenbergh, Johan Jozef Rosa Maria
Data de Publicação: 2004
Outros Autores: Santana, Gisélia, D' Oliveira Junior, Argemiro, Santos Junior, Anibal F, Costa, Carlos Henrique Nery, Carvalho Filho, Edgar Marcelino, Barral, Aldina Maria Prado, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/3261
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
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spelling Van Weyenbergh, Johan Jozef Rosa MariaSantana, GiséliaD' Oliveira Junior, ArgemiroSantos Junior, Anibal FCosta, Carlos Henrique NeryCarvalho Filho, Edgar MarcelinoBarral, Aldina Maria PradoBarral Netto, Manoel2011-10-18T17:08:48Z2011-10-18T17:08:48Z2004VAN WWYENBERGH, J. et al. Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study. BMC Infections Disease, v. 4, p. 50, nov. 2004.1471-2334https://www.arca.fiocruz.br/handle/icict/326110.1186/1471-2334-4-50engZinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Hospital Universitário Professor Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Química. Salvador, BA, Brasil.Universidade Federal do Piauí. Infectious Disease Hospital. Teresina, PI, Brasil.Institute for Immunological Investigation. Instituto do Milênio. Sao Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.The process of elimination of intracellular pathogens, such as Leishmania, requires a Th1 type immune response, whereas a dominant Th2 response leads to exacerbated disease. Experimental human zinc deficiency decreases Th1 but not Th2 immune response. We investigated if zinc and copper levels differ in different clinical forms of leishmaniasis, and if these trace metals might be involved in the immune response towards the parasite.Blood was collected from 31 patients with either localized cutaneous (LCL), mucosal (ML) or visceral (VL) leishmaniasis, as well as from 25 controls from endemic and non-endemic areas. Anti-Leishmania humoral and cellular immune response were evaluated by quantifying specific plasma IgG, lymphoproliferation and cytokine production, respectively. Plasma levels of Cu and Zn were quantified by atomic absorption spectrophotometry.A significant decrease in plasma Zn was observed in all three patient groups (p < 0.01 for LCL and ML, p < 0.001 for VL), as compared to controls, but only VL (7/10) and ML (1/7) patients displayed overt Zn deficiency. Plasma Cu was increased in LCL and VL (p < 0.001) but not in ML, and was strongly correlated to anti-Leishmania IgG (Spearman r = 0.65, p = 0.0028). Cu/Zn ratios were highest in patients with deficient cellular (VL<LCL>ML) immune response. Ex vivo production of parasite-induced IFN-gamma was negatively correlated to plasma Cu levels in LCL (r = -0.57, p = 0.01). In vitro, increased Cu levels inhibited IFN-gamma production.1. Zn deficiency in VL and ML indicate possible therapeutic administration of Zn in these severe forms of leishmaniasis. 2. Plasma Cu positively correlates to humoral immune response across patient groups. 3. Environmentally or genetically determined increases in Cu levels might augment susceptibility to infection with intracellular pathogens, by causing a decrease in IFN-gamma production.Anticorpos AntiprotozoáriosEstudos de Casos e ControlesCobreImunidade CelularImunoglobulina GInterferon gamaLeishmaniaLeishmanioseAtivação LinfocíticaCélulas Th1Células Th2ZincoAdolescenteAdultoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALVan Weyenbergh J Zinc copper imbalance reflects....pdfVan Weyenbergh J Zinc copper imbalance reflects....pdfapplication/pdf283895https://www.arca.fiocruz.br/bitstream/icict/3261/1/Van%20Weyenbergh%20J%20Zinc%20copper%20imbalance%20reflects....pdfbc9a1606efa0c971b0d550c922b8e5efMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3261/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTVan Weyenbergh J Zinc copper imbalance reflects....pdf.txtVan Weyenbergh J Zinc copper imbalance reflects....pdf.txtExtracted texttext/plain27498https://www.arca.fiocruz.br/bitstream/icict/3261/5/Van%20Weyenbergh%20J%20Zinc%20copper%20imbalance%20reflects....pdf.txtd5bc204e272384b1a12b93e9b6708271MD55THUMBNAILVan Weyenbergh J Zinc copper imbalance reflects....pdf.jpgVan Weyenbergh J Zinc copper imbalance reflects....pdf.jpgGenerated Thumbnailimage/jpeg2266https://www.arca.fiocruz.br/bitstream/icict/3261/4/Van%20Weyenbergh%20J%20Zinc%20copper%20imbalance%20reflects....pdf.jpg4990592b689eb1483b1894afcbe9299fMD54icict/32612023-03-15 14:33:18.209oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
title Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
spellingShingle Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
Van Weyenbergh, Johan Jozef Rosa Maria
Anticorpos Antiprotozoários
Estudos de Casos e Controles
Cobre
Imunidade Celular
Imunoglobulina G
Interferon gama
Leishmania
Leishmaniose
Ativação Linfocítica
Células Th1
Células Th2
Zinco
Adolescente
Adulto
title_short Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
title_full Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
title_fullStr Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
title_full_unstemmed Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
title_sort Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study
author Van Weyenbergh, Johan Jozef Rosa Maria
author_facet Van Weyenbergh, Johan Jozef Rosa Maria
Santana, Gisélia
D' Oliveira Junior, Argemiro
Santos Junior, Anibal F
Costa, Carlos Henrique Nery
Carvalho Filho, Edgar Marcelino
Barral, Aldina Maria Prado
Barral Netto, Manoel
author_role author
author2 Santana, Gisélia
D' Oliveira Junior, Argemiro
Santos Junior, Anibal F
Costa, Carlos Henrique Nery
Carvalho Filho, Edgar Marcelino
Barral, Aldina Maria Prado
Barral Netto, Manoel
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Van Weyenbergh, Johan Jozef Rosa Maria
Santana, Gisélia
D' Oliveira Junior, Argemiro
Santos Junior, Anibal F
Costa, Carlos Henrique Nery
Carvalho Filho, Edgar Marcelino
Barral, Aldina Maria Prado
Barral Netto, Manoel
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antiprotozoários
Estudos de Casos e Controles
Cobre
Imunidade Celular
Imunoglobulina G
Interferon gama
Leishmania
Leishmaniose
Ativação Linfocítica
Células Th1
Células Th2
Zinco
Adolescente
Adulto
topic Anticorpos Antiprotozoários
Estudos de Casos e Controles
Cobre
Imunidade Celular
Imunoglobulina G
Interferon gama
Leishmania
Leishmaniose
Ativação Linfocítica
Células Th1
Células Th2
Zinco
Adolescente
Adulto
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2011-10-18T17:08:48Z
dc.date.available.fl_str_mv 2011-10-18T17:08:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VAN WWYENBERGH, J. et al. Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study. BMC Infections Disease, v. 4, p. 50, nov. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/3261
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2334-4-50
identifier_str_mv VAN WWYENBERGH, J. et al. Zinc/copper imbalance reflects immune dysfunction in human leishmaniasis: an ex vivo and in vitro study. BMC Infections Disease, v. 4, p. 50, nov. 2004.
1471-2334
10.1186/1471-2334-4-50
url https://www.arca.fiocruz.br/handle/icict/3261
dc.language.iso.fl_str_mv eng
language eng
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instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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