Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor

Detalhes bibliográficos
Autor(a) principal: Shoemaker, Charles B
Data de Publicação: 1992
Outros Autores: Ramachandran, Hema, Landa, Abraham, Reis, Mitermayer Galvão dos, Stein, Lincoln D
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7581
Resumo: Department of Tropical Public Health. Harvard School of Public Health. Boston, MA, USA
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spelling Shoemaker, Charles BRamachandran, HemaLanda, AbrahamReis, Mitermayer Galvão dosStein, Lincoln D2014-05-06T11:53:58Z2014-05-06T11:53:58Z1992SHOEMAKER, C. et al. Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor. Molecular and Biochemical Parasitology, v. 53, n. 1-2, p. 17-32, 1992.0166-6851https://www.arca.fiocruz.br/handle/icict/7581engElsevier Science Publishers B.VAlternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptorinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Tropical Public Health. Harvard School of Public Health. Boston, MA, USADepartment of Tropical Public Health. Harvard School of Public Health. Boston, MA, USADepartment of Immunology. Inst. de Investigaciones Biomédicas. UNAM. México, D.F, MexicoFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilDeptartment of Pathology. Brigham and Women's Hospital. Harvard Medical School. Boston, MA, USAThe complete coding DNA for a Schistosoma mansoni homologue of the epidermal growth factor receptor (SER) was characterized from cDNA clones obtained by homology to the tyrosine kinase domain of erbB. The DNA sequence predicts a 200-kDa translation product that contains a secretory leader, a cysteine-rich extracellular domain, a hydrophobic transmembrane sequence, and an intracellular tyrosine kinase domain. The SER transcript is present in cercariae and adult schistosomes. In addition to SER transcripts, schistosomes produce at least 3 variant transcripts encoding truncated SER products that include the secretory leader and a small portion of the extracellular domain followed by short sequences of unrelated, Cterminal amino acids. Based on these sequences, 2 of the variant mRNAs (class 2 and 5) appear to encode soluble, secreted proteins while one (class 4) encodes an SER variant protein with a hydrophobic C-terminus that may serve as a membrane anchor. Class 2 SER variant transcripts are present at levels comparable to SER transcripts in adult worms but are not detected in cercariae. Class 4 and 5 SER variant transcripts are also found within adult worms but at lower levels. Genomic cloning and characterization demonstrate that the variant SER transcripts arise through alternative splicing of the SER geneSchistosomeSchistosoma mansoniEGFEGF receptorAlternative splicingMembrane proteinProcessamento de RNAReceptor do Fator de Crescimento Epidérmico/genéticaSchistosoma mansoni/genéticaSequência de AminoácidosAnimaisSequência de BasesClonagem MolecularDNA/genéticaProteínas de Helminto/genéticaDados de Sequência MolecularRNA Mensageiro/genéticaHomologia de Sequência do Ácido NucleicoTranscrição Genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALShoemaker C Alternative Splicing of the....pdfShoemaker C Alternative Splicing of the....pdfapplication/pdf1252606https://www.arca.fiocruz.br/bitstream/icict/7581/1/Shoemaker%20C%20Alternative%20Splicing%20of%20the....pdfcc037e038f1e1dd1c8d3e7f7de5f334cMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7581/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTShoemaker C Alternative Splicing of the....pdf.txtShoemaker C Alternative Splicing of the....pdf.txtExtracted texttext/plain59480https://www.arca.fiocruz.br/bitstream/icict/7581/5/Shoemaker%20C%20Alternative%20Splicing%20of%20the....pdf.txtd139bc080b70ea64f53f021c2759de99MD55THUMBNAILShoemaker C Alternative Splicing of the....pdf.jpgShoemaker C Alternative Splicing of the....pdf.jpgGenerated Thumbnailimage/jpeg2484https://www.arca.fiocruz.br/bitstream/icict/7581/4/Shoemaker%20C%20Alternative%20Splicing%20of%20the....pdf.jpg5c6041e5ca4a945d88843e3ee4c8b576MD54icict/75812023-03-15 14:33:18.73oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:18Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
title Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
spellingShingle Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
Shoemaker, Charles B
Schistosome
Schistosoma mansoni
EGF
EGF receptor
Alternative splicing
Membrane protein
Processamento de RNA
Receptor do Fator de Crescimento Epidérmico/genética
Schistosoma mansoni/genética
Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA/genética
Proteínas de Helminto/genética
Dados de Sequência Molecular
RNA Mensageiro/genética
Homologia de Sequência do Ácido Nucleico
Transcrição Genética
title_short Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
title_full Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
title_fullStr Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
title_full_unstemmed Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
title_sort Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor
author Shoemaker, Charles B
author_facet Shoemaker, Charles B
Ramachandran, Hema
Landa, Abraham
Reis, Mitermayer Galvão dos
Stein, Lincoln D
author_role author
author2 Ramachandran, Hema
Landa, Abraham
Reis, Mitermayer Galvão dos
Stein, Lincoln D
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Shoemaker, Charles B
Ramachandran, Hema
Landa, Abraham
Reis, Mitermayer Galvão dos
Stein, Lincoln D
dc.subject.en.pt_BR.fl_str_mv Schistosome
Schistosoma mansoni
EGF
EGF receptor
Alternative splicing
Membrane protein
topic Schistosome
Schistosoma mansoni
EGF
EGF receptor
Alternative splicing
Membrane protein
Processamento de RNA
Receptor do Fator de Crescimento Epidérmico/genética
Schistosoma mansoni/genética
Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA/genética
Proteínas de Helminto/genética
Dados de Sequência Molecular
RNA Mensageiro/genética
Homologia de Sequência do Ácido Nucleico
Transcrição Genética
dc.subject.decs.pt_BR.fl_str_mv Processamento de RNA
Receptor do Fator de Crescimento Epidérmico/genética
Schistosoma mansoni/genética
Sequência de Aminoácidos
Animais
Sequência de Bases
Clonagem Molecular
DNA/genética
Proteínas de Helminto/genética
Dados de Sequência Molecular
RNA Mensageiro/genética
Homologia de Sequência do Ácido Nucleico
Transcrição Genética
description Department of Tropical Public Health. Harvard School of Public Health. Boston, MA, USA
publishDate 1992
dc.date.issued.fl_str_mv 1992
dc.date.accessioned.fl_str_mv 2014-05-06T11:53:58Z
dc.date.available.fl_str_mv 2014-05-06T11:53:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SHOEMAKER, C. et al. Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor. Molecular and Biochemical Parasitology, v. 53, n. 1-2, p. 17-32, 1992.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7581
dc.identifier.issn.none.fl_str_mv 0166-6851
identifier_str_mv SHOEMAKER, C. et al. Alternative splicing of the Schistosoma mansoni gene encoding a homologue of epidermal growth factor receptor. Molecular and Biochemical Parasitology, v. 53, n. 1-2, p. 17-32, 1992.
0166-6851
url https://www.arca.fiocruz.br/handle/icict/7581
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Science Publishers B.V
publisher.none.fl_str_mv Elsevier Science Publishers B.V
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