Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy

Detalhes bibliográficos
Autor(a) principal: Souza, Beatriz Junqueira de
Data de Publicação: 2022
Outros Autores: Mendes, Mayara Abud, Silva, Gilberto Marcelo Sperandio da, Sammarco-Rosa, Patrícia, Moraes, Milton Ozorio de, Jardim, Marcia Rodrigues, Sarno, Euzenir Nunes, Pinheiro, Roberto Olmo, Mietto, Bruno Siqueira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53551
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
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spelling Souza, Beatriz Junqueira deMendes, Mayara AbudSilva, Gilberto Marcelo Sperandio daSammarco-Rosa, PatríciaMoraes, Milton Ozorio deJardim, Marcia RodriguesSarno, Euzenir NunesPinheiro, Roberto OlmoMietto, Bruno Siqueira2022-06-27T23:11:08Z2022-06-27T23:11:08Z2022SOUZA, Beatriz Junqueira et al. Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy. Frontiers in Medicine, v. 9, p. 1-12, 2022.2296-858Xhttps://www.arca.fiocruz.br/handle/icict/5355110.3389/fmed.2022.861586engFrontiers MediaGene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Doença de Chagas. Rio de Janeiro, RJ, Brasil.Lauro de Souza Lima Institute. Laboratory Animal House. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Federal University of Juiz de Fora. Institute of Biological Sciences. Laboratory of Cell Biology. Juiz de Fora, MG, Brazil.Peripheral neuropathy is the main cause of physical disability in leprosy patients. Importantly, the extension and pattern of peripheral damage has been linked to how the host cell will respond against Mycobacterium leprae (M. leprae) infection, in particular, how the pathogen will establish infection in Schwann cells. Interestingly, viable and dead M. leprae have been linked to neuropathology of leprosy by distinct mechanisms. While viable M. leprae promotes transcriptional modifications that allow the bacteria to survive through the use of the host cell's internal machinery and the subvert of host metabolites, components of the dead bacteria are associated with the generation of a harmful nerve microenvironment. Therefore, understanding the pathognomonic characteristics mediated by viable and dead M. leprae are essential for elucidating leprosy disease and its associated reactional episodes. Moreover, the impact of the viable and dead bacteria in Schwann cells is largely unknown and their gene signature profiling has, as yet, been poorly explored. In this study, we analyzed the early differences in the expression profile of genes involved in peripheral neuropathy, dedifferentiation and plasticity, neural regeneration, and inflammation in human Schwann cells challenged with viable and dead M. leprae. We substantiated our findings by analyzing this genetic profiling in human nerve biopsies of leprosy and non-leprosy patients, with accompanied histopathological analysis. We observed that viable and dead bacteria distinctly modulate Schwann cell genes, with emphasis to viable bacilli upregulating transcripts related to glial cell plasticity, dedifferentiation and anti-inflammatory profile, while dead bacteria affected genes involved in neuropathy and pro-inflammatory response. In addition, dead bacteria also upregulated genes associated with nerve support, which expression profile was similar to those obtained from leprosy nerve biopsies. These findings suggest that early exposure to viable and dead bacteria may provoke Schwann cells to behave differentially, with far-reaching implications for the ongoing neuropathy seen in leprosy patients, where a mixture of active and non-active bacteria are found in the nerve microenvironment.Mycobacterium lepraeSchwann cellHost-pathogen interactionLeprosyPeripheral nervous systeminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/53551/1/license.txt73c2438d9038594f05d706f987b602c3MD51ORIGINALGene_Gilberto_Silva_etal_INI_2022.pdfGene_Gilberto_Silva_etal_INI_2022.pdfapplication/pdf2049167https://www.arca.fiocruz.br/bitstream/icict/53551/2/Gene_Gilberto_Silva_etal_INI_2022.pdf044fb255ab92d8a99735a05d373e2a95MD52icict/535512023-04-28 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dc.title.pt_BR.fl_str_mv Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
title Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
spellingShingle Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
Souza, Beatriz Junqueira de
Mycobacterium leprae
Schwann cell
Host-pathogen interaction
Leprosy
Peripheral nervous system
title_short Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
title_full Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
title_fullStr Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
title_full_unstemmed Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
title_sort Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy
author Souza, Beatriz Junqueira de
author_facet Souza, Beatriz Junqueira de
Mendes, Mayara Abud
Silva, Gilberto Marcelo Sperandio da
Sammarco-Rosa, Patrícia
Moraes, Milton Ozorio de
Jardim, Marcia Rodrigues
Sarno, Euzenir Nunes
Pinheiro, Roberto Olmo
Mietto, Bruno Siqueira
author_role author
author2 Mendes, Mayara Abud
Silva, Gilberto Marcelo Sperandio da
Sammarco-Rosa, Patrícia
Moraes, Milton Ozorio de
Jardim, Marcia Rodrigues
Sarno, Euzenir Nunes
Pinheiro, Roberto Olmo
Mietto, Bruno Siqueira
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Beatriz Junqueira de
Mendes, Mayara Abud
Silva, Gilberto Marcelo Sperandio da
Sammarco-Rosa, Patrícia
Moraes, Milton Ozorio de
Jardim, Marcia Rodrigues
Sarno, Euzenir Nunes
Pinheiro, Roberto Olmo
Mietto, Bruno Siqueira
dc.subject.en.pt_BR.fl_str_mv Mycobacterium leprae
Schwann cell
Host-pathogen interaction
Leprosy
Peripheral nervous system
topic Mycobacterium leprae
Schwann cell
Host-pathogen interaction
Leprosy
Peripheral nervous system
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-06-27T23:11:08Z
dc.date.available.fl_str_mv 2022-06-27T23:11:08Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Beatriz Junqueira et al. Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy. Frontiers in Medicine, v. 9, p. 1-12, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53551
dc.identifier.issn.pt_BR.fl_str_mv 2296-858X
dc.identifier.doi.none.fl_str_mv 10.3389/fmed.2022.861586
identifier_str_mv SOUZA, Beatriz Junqueira et al. Gene Expression Profile of Mycobacterium leprae Contribution in the Pathology of Leprosy Neuropathy. Frontiers in Medicine, v. 9, p. 1-12, 2022.
2296-858X
10.3389/fmed.2022.861586
url https://www.arca.fiocruz.br/handle/icict/53551
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/53551/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/53551/2/Gene_Gilberto_Silva_etal_INI_2022.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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