High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Faria, Angélica Rosa
Data de Publicação: 2011
Outros Autores: Andrade, Hélida Monteiro de, Franco, Miriam Maria Silva Costa, Giusta, Mário S., Penido, Marcus L. O., Grimaldi Filho, Gabriel, Gazzinelli, Ricardo Tostes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8680
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
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spelling Faria, Angélica RosaAndrade, Hélida Monteiro deFranco, Miriam Maria Silva CostaGiusta, Mário S.Penido, Marcus L. O.Grimaldi Filho, GabrielGazzinelli, Ricardo Tostes2014-10-28T11:49:44Z2014-10-28T11:49:44Z2011FARIA, Angélica et al. High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. Plos Neglect Trop D, 5(9): e1310.1935-2735https://www.arca.fiocruz.br/handle/icict/868010.1371/journal.pntd.0001310engPublic Library of ScienceHigh-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil/University of Massachusetts Medical School. Division of Infectious Diseases and Immunology Worcester, MA, United States of America/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilBackground: Visceral leishmaniasis is the most severe form of leishmaniasis. Approximately 20% of zoonotic human visceral leishmaniasis worldwide is caused by Leishmania infantum, which is also known as Leishmania chagasi in Latin America, and disease incidence is increasing in urban and peri-urban areas of the tropics. In this form of disease, dogs are the main reservoirs. Diagnostic methods used to identify Leishmania infected animals are not able to detect all of the infected ones, which can compromise the effectiveness of disease control. Therefore, to contribute to the improvement of diagnostic methods for canine visceral leishmaniasis (CVL), we aimed to identify and test novel antigens using high-throughput analysis. Methodology/Principal Findings: Immunodominant proteins from L. infantum were mapped in silico to predict B cell epitopes, and the 360 predicted peptides were synthesized on cellulose membranes. Immunoassays were used to select the most reactive peptides, which were then investigated with canine sera. Next, the 10 most reactive peptides were synthesized using solid phase peptide synthesis protocol and tested using ELISA. The sensitivity and specificity of these peptides were also compared to the EIE-LVC Bio-Manguinhos kit, which is recommended by the Brazilian Ministry of Health for use in leishmaniasis control programs. The sensitivity and specificity of the selected synthesized peptides was as high as 88.70% and 95.00%, respectively, whereas the EIE-LVC kit had a sensitivity of 13.08% and 100.00% of specificity. Although the tests based on synthetic peptides were able to diagnose up to 94.80% of asymptomatic dogs with leishmaniasis, the EIE-LVC kit failed to detect the disease in any of the infected asymptomatic dogs. Conclusions/Significance: Our study shows that ELISA using synthetic peptides is a technique with great potential for diagnosing CVL; furthermore, the use of these peptides in other diagnostic methodologies, such as immunochromatographic tests, could be beneficial to CVL control programs.Diagnostic medicineEnzyme-linked immunoassaysLeishmaniasisParasitic diseasesPeptide mappingPeptide synthesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8680/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALHigh-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis.pdfHigh-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis.pdfapplication/pdf447092https://www.arca.fiocruz.br/bitstream/icict/8680/2/High-throughput%20analysis%20of%20synthetic%20peptides%20for%20the%20immunodiagnosis%20of%20canine%20visceral%20leishmaniasis.pdf1446b7529d24a3c40e0847429d6f11abMD52TEXTHigh-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis.pdf.txtHigh-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis.pdf.txtExtracted texttext/plain48391https://www.arca.fiocruz.br/bitstream/icict/8680/3/High-throughput%20analysis%20of%20synthetic%20peptides%20for%20the%20immunodiagnosis%20of%20canine%20visceral%20leishmaniasis.pdf.txtd149163460f0c10ce71df504a45f2e70MD53icict/86802019-06-19 10:12:47.92oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
title High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
spellingShingle High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
Faria, Angélica Rosa
Diagnostic medicine
Enzyme-linked immunoassays
Leishmaniasis
Parasitic diseases
Peptide mapping
Peptide synthesis
title_short High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
title_full High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
title_fullStr High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
title_full_unstemmed High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
title_sort High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis
author Faria, Angélica Rosa
author_facet Faria, Angélica Rosa
Andrade, Hélida Monteiro de
Franco, Miriam Maria Silva Costa
Giusta, Mário S.
Penido, Marcus L. O.
Grimaldi Filho, Gabriel
Gazzinelli, Ricardo Tostes
author_role author
author2 Andrade, Hélida Monteiro de
Franco, Miriam Maria Silva Costa
Giusta, Mário S.
Penido, Marcus L. O.
Grimaldi Filho, Gabriel
Gazzinelli, Ricardo Tostes
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Faria, Angélica Rosa
Andrade, Hélida Monteiro de
Franco, Miriam Maria Silva Costa
Giusta, Mário S.
Penido, Marcus L. O.
Grimaldi Filho, Gabriel
Gazzinelli, Ricardo Tostes
dc.subject.en.pt_BR.fl_str_mv Diagnostic medicine
Enzyme-linked immunoassays
Leishmaniasis
Parasitic diseases
Peptide mapping
Peptide synthesis
topic Diagnostic medicine
Enzyme-linked immunoassays
Leishmaniasis
Parasitic diseases
Peptide mapping
Peptide synthesis
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-28T11:49:44Z
dc.date.available.fl_str_mv 2014-10-28T11:49:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FARIA, Angélica et al. High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. Plos Neglect Trop D, 5(9): e1310.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8680
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001310
identifier_str_mv FARIA, Angélica et al. High-throughput analysis of synthetic peptides for the immunodiagnosis of canine visceral leishmaniasis. Plos Neglect Trop D, 5(9): e1310.
1935-2735
10.1371/journal.pntd.0001310
url https://www.arca.fiocruz.br/handle/icict/8680
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/8680/1/license.txt
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