Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay

Detalhes bibliográficos
Autor(a) principal: Costa, Míriam Maria
Data de Publicação: 2012
Outros Autores: Penido, Marcus Luiz de Oliveira, Gazzinelli, Ricardo Tostes, Santos, Mariana Silva dos, Doro, Daniel, Fernandes, Ana Paula, Freitas, Eloísa de, Michalick, Marilene Susan Marques, Grimaldi, Gabriel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7392
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
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spelling Costa, Míriam MariaPenido, Marcus Luiz de OliveiraGazzinelli, Ricardo TostesSantos, Mariana Silva dosDoro, DanielFernandes, Ana PaulaFreitas, Eloísa deMichalick, Marilene Susan MarquesGrimaldi, Gabriel2014-03-11T13:23:20Z2014-03-11T13:23:20Z2012COSTA, Míriam Maria et al. Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay. Plos Neglected Tropical Diseases, v. 6, p. 1-9, 2012.1935-2727https://www.arca.fiocruz.br/handle/icict/739210.1371/journal.pntd.0001622engPublic library of ScienceImproved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil / University of Massachusetts Medical School. Division of Infectious Diseases and Immunology. Worcester, Ma, USA.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Background: Zoonotic visceral leishmaniasis (VL) is a severe infectious disease caused by protozoan parasites of the genus Leishmania and the domestic dogs are the main urban parasite reservoir hosts. In Brazil, indirect fluorescence antibody tests (IFAT) and indirect enzyme linked immunosorbent assay (ELISA) using promastigote extracts are widely used in epidemiological surveys. However, their sensitivity and specificity have often been compromised by the use of complex mixtures of antigens, which reduces their accuracy allowing the maintenance of infected animals that favors transmission to humans. In this context, the use of combinations of defined peptides appears favorable. Therefore, they were tested by combinations of five peptides derived from the previously described Leishmania diagnostic antigens A2, NH, LACK and K39. Methodology/Principal Findings : Combinations of peptides derived A2, NH, LACK and K39 antigens were used in ELISA with sera from 44 human patients and 106 dogs. Improved sensitivities and specificities, close to 100%, were obtained for both sera of patients and dogs. Moreover, high sensitivity and specificity were observed even for canine sera presenting low IFAT anti-Leishmania antibody titers or from asymptomatic animals. Conclusions/Significance : The use of combinations of B cell predicted synthetic peptides derived from antigens A2, NH, LACK and K39 may provide an alternative for improved sensitivities and specificities for immunodiagnostic assays of VL.AntibodiesDiagnostic medicineDogsEnzyme-linked immunoassaysKala-azarLeishmaniasisParasitic diseasesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL. Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay.pdf. 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dc.title.pt_BR.fl_str_mv Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
title Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
spellingShingle Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
Costa, Míriam Maria
Antibodies
Diagnostic medicine
Dogs
Enzyme-linked immunoassays
Kala-azar
Leishmaniasis
Parasitic diseases
title_short Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
title_full Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
title_fullStr Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
title_full_unstemmed Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
title_sort Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay
author Costa, Míriam Maria
author_facet Costa, Míriam Maria
Penido, Marcus Luiz de Oliveira
Gazzinelli, Ricardo Tostes
Santos, Mariana Silva dos
Doro, Daniel
Fernandes, Ana Paula
Freitas, Eloísa de
Michalick, Marilene Susan Marques
Grimaldi, Gabriel
author_role author
author2 Penido, Marcus Luiz de Oliveira
Gazzinelli, Ricardo Tostes
Santos, Mariana Silva dos
Doro, Daniel
Fernandes, Ana Paula
Freitas, Eloísa de
Michalick, Marilene Susan Marques
Grimaldi, Gabriel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Míriam Maria
Penido, Marcus Luiz de Oliveira
Gazzinelli, Ricardo Tostes
Santos, Mariana Silva dos
Doro, Daniel
Fernandes, Ana Paula
Freitas, Eloísa de
Michalick, Marilene Susan Marques
Grimaldi, Gabriel
dc.subject.en.pt_BR.fl_str_mv Antibodies
Diagnostic medicine
Dogs
Enzyme-linked immunoassays
Kala-azar
Leishmaniasis
Parasitic diseases
topic Antibodies
Diagnostic medicine
Dogs
Enzyme-linked immunoassays
Kala-azar
Leishmaniasis
Parasitic diseases
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-03-11T13:23:20Z
dc.date.available.fl_str_mv 2014-03-11T13:23:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Míriam Maria et al. Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay. Plos Neglected Tropical Diseases, v. 6, p. 1-9, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7392
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001622
identifier_str_mv COSTA, Míriam Maria et al. Improved canine and human visceral leishmaniasis immunodiagnosis using combinations of synthetic peptides in enzyme-linked immunosorbent assay. Plos Neglected Tropical Diseases, v. 6, p. 1-9, 2012.
1935-2727
10.1371/journal.pntd.0001622
url https://www.arca.fiocruz.br/handle/icict/7392
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public library of Science
publisher.none.fl_str_mv Public library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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