The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1371/journal.pntd.0001779 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7850 |
Resumo: | Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil |
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Santos, Sara Lopes dosFreitas, Leandro MartinsLobo, Francisco PereiraLuiz, Gabriela Flávia RodriguesMendes, Tiago Antônio de OliveiraOliveira, Anny Carolline SilvaChiari, ÉglerFujiwara, Ricardo ToshioBartholomeu, Daniella CastanheiraAndrade, Luciana OliveiraGazzinelli, Ricardo TostesTeixeira, Santuza Maria Ribeiro2014-06-27T17:21:27Z2014-06-27T17:21:27Z2012SANTOS, Sara Lopes dos et al. The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e17791932-6203https://www.arca.fiocruz.br/handle/icict/785010.1371/journal.pntd.0001779engPublic Library of ScienceThe MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilBackground: Trypanosoma cruzi is the etiological agent of Chagas disease, a debilitating illness that affects millions of people in the Americas. A major finding of the T. cruzi genome project was the discovery of a novel multigene family composed of approximately 1,300 genes that encode mucin-associated surface proteins (MASPs). The high level of polymorphism of the MASP family associated with its localization at the surface of infective forms of the parasite suggests that MASP participates in host–parasite interactions. We speculate that the large repertoire of MASP sequences may contribute to the ability of T. cruzi to infect several host cell types and/or participate in host immune evasion mechanisms. Methods: By sequencing seven cDNA libraries, we analyzed the MASP expression profile in trypomastigotes derived from distinct host cells and after sequential passages in acutely infected mice. Additionally, to investigate the MASP antigenic profile, we performed B-cell epitope prediction on MASP proteins and designed a MASP-specific peptide array with 110 putative epitopes, which was screened with sera from acutely infected mice. Findings and Conclusions: We observed differential expression of a few MASP genes between trypomastigotes derived from epithelial and myoblast cell lines. The more pronounced MASP expression changes were observed between bloodstream and tissue-culture trypomastigotes and between bloodstream forms from sequential passages in acutely infected mice. Moreover, we demonstrated that different MASP members were expressed during the acute T. cruzi infection and constitute parasite antigens that are recognized by IgG and IgM antibodies. We also found that distinct MASP peptides could trigger different antibody responses and that the antibody level against a given peptide may vary after sequential passages in mice. We speculate that changes in the large repertoire of MASP antigenic peptides during an infection may contribute to the evasion of host immune responses during the acute phase of Chagas disease.Bloodstream infectionsEnzyme-linked immunoassaysGene expressionHost cells Parasitic diseasesPeptide synthesisTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALThe MASP family of Trypanosoma cruzi.pdfThe MASP family of Trypanosoma cruzi.pdfapplication/pdf3691710https://www.arca.fiocruz.br/bitstream/icict/7850/1/The%20MASP%20family%20of%20Trypanosoma%20cruzi.pdf7b9f1756920e58e2d9da47a57f992808MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7850/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTThe MASP family of Trypanosoma cruzi.pdf.txtThe MASP family of Trypanosoma cruzi.pdf.txtExtracted texttext/plain14https://www.arca.fiocruz.br/bitstream/icict/7850/3/The%20MASP%20family%20of%20Trypanosoma%20cruzi.pdf.txt06b7e51e8fc077b8c75076712e4dd2b3MD53icict/78502018-04-06 08:43:53.636oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| title |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| spellingShingle |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection Santos, Sara Lopes dos Bloodstream infections Enzyme-linked immunoassays Gene expression Host cells Parasitic diseases Peptide synthesis Trypanosoma cruzi |
| title_short |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| title_full |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| title_fullStr |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| title_full_unstemmed |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| title_sort |
The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection |
| author |
Santos, Sara Lopes dos |
| author_facet |
Santos, Sara Lopes dos Freitas, Leandro Martins Lobo, Francisco Pereira Luiz, Gabriela Flávia Rodrigues Mendes, Tiago Antônio de Oliveira Oliveira, Anny Carolline Silva Chiari, Égler Fujiwara, Ricardo Toshio Bartholomeu, Daniella Castanheira Andrade, Luciana Oliveira Gazzinelli, Ricardo Tostes Teixeira, Santuza Maria Ribeiro |
| author_role |
author |
| author2 |
Freitas, Leandro Martins Lobo, Francisco Pereira Luiz, Gabriela Flávia Rodrigues Mendes, Tiago Antônio de Oliveira Oliveira, Anny Carolline Silva Chiari, Égler Fujiwara, Ricardo Toshio Bartholomeu, Daniella Castanheira Andrade, Luciana Oliveira Gazzinelli, Ricardo Tostes Teixeira, Santuza Maria Ribeiro |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Sara Lopes dos Freitas, Leandro Martins Lobo, Francisco Pereira Luiz, Gabriela Flávia Rodrigues Mendes, Tiago Antônio de Oliveira Oliveira, Anny Carolline Silva Chiari, Égler Fujiwara, Ricardo Toshio Bartholomeu, Daniella Castanheira Andrade, Luciana Oliveira Gazzinelli, Ricardo Tostes Teixeira, Santuza Maria Ribeiro |
| dc.subject.en.pt_BR.fl_str_mv |
Bloodstream infections Enzyme-linked immunoassays Gene expression Host cells Parasitic diseases Peptide synthesis Trypanosoma cruzi |
| topic |
Bloodstream infections Enzyme-linked immunoassays Gene expression Host cells Parasitic diseases Peptide synthesis Trypanosoma cruzi |
| description |
Universidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, Brazil |
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2012 |
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2012 |
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2014-06-27T17:21:27Z |
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2014-06-27T17:21:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SANTOS, Sara Lopes dos et al. The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1779 |
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https://www.arca.fiocruz.br/handle/icict/7850 |
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1932-6203 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0001779 |
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SANTOS, Sara Lopes dos et al. The MASP family of Trypanosoma cruzi: changes in gene expression and antigenic profile during the acute phase of experimental infection. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1779 1932-6203 10.1371/journal.pntd.0001779 |
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