Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts

Detalhes bibliográficos
Autor(a) principal: Pires, Renata de Cássia
Data de Publicação: 2017
Outros Autores: Melo, Myllena de Fátima Alheiros Dias de, Barbosa, Raquel da Hora, Roque, André Luiz Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20073
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.
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spelling Pires, Renata de CássiaMelo, Myllena de Fátima Alheiros Dias deBarbosa, Raquel da HoraRoque, André Luiz Rodrigues2017-07-13T13:47:25Z2017-07-13T13:47:25Z2017PIRES, Renata de Cássia; et al. Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts. PLoS ONE, v.12, n.3, e0173922, 12p, Mar. 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2007310.1371/journal.pone.0173922engPublic Library of ScienceDNA de CinetoplastoLeishmaniaReação em Cadeia da Polimerase MultiplexEspécies de mamíferos selvagensLeishmania sp. kDNAMultiplex PCRwild mammal speciesMultiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hostsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto Biomédico. Centro de Ciências Médicas. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.The PCR assays usually employed for Leishmania diagnosis does not simultaneously detect a constitutive gene that would certify the viability of the DNA sample. We present a multiplex PCR approach for the simultaneous diagnosis of the Leishmania sp. kDNA fragment and a catalytic domain segment of a conserved region of the mammalian gapdh gene.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20073/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrenata_pires_etal_IOC_2017.pdfrenata_pires_etal_IOC_2017.pdfapplication/pdf2389849https://www.arca.fiocruz.br/bitstream/icict/20073/2/renata_pires_etal_IOC_2017.pdf52fabd62d73165e3c834a186be781fb0MD52TEXTrenata_pires_etal_IOC_2017.pdf.txtrenata_pires_etal_IOC_2017.pdf.txtExtracted texttext/plain38584https://www.arca.fiocruz.br/bitstream/icict/20073/3/renata_pires_etal_IOC_2017.pdf.txte828c83520989338ca2ca28516064024MD53icict/200732018-08-15 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dc.title.pt_BR.fl_str_mv Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
title Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
spellingShingle Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
Pires, Renata de Cássia
DNA de Cinetoplasto
Leishmania
Reação em Cadeia da Polimerase Multiplex
Espécies de mamíferos selvagens
Leishmania sp. kDNA
Multiplex PCR
wild mammal species
title_short Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
title_full Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
title_fullStr Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
title_full_unstemmed Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
title_sort Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts
author Pires, Renata de Cássia
author_facet Pires, Renata de Cássia
Melo, Myllena de Fátima Alheiros Dias de
Barbosa, Raquel da Hora
Roque, André Luiz Rodrigues
author_role author
author2 Melo, Myllena de Fátima Alheiros Dias de
Barbosa, Raquel da Hora
Roque, André Luiz Rodrigues
author2_role author
author
author
dc.contributor.author.fl_str_mv Pires, Renata de Cássia
Melo, Myllena de Fátima Alheiros Dias de
Barbosa, Raquel da Hora
Roque, André Luiz Rodrigues
dc.subject.other.pt_BR.fl_str_mv DNA de Cinetoplasto
Leishmania
Reação em Cadeia da Polimerase Multiplex
Espécies de mamíferos selvagens
topic DNA de Cinetoplasto
Leishmania
Reação em Cadeia da Polimerase Multiplex
Espécies de mamíferos selvagens
Leishmania sp. kDNA
Multiplex PCR
wild mammal species
dc.subject.en.pt_BR.fl_str_mv Leishmania sp. kDNA
Multiplex PCR
wild mammal species
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ. Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-13T13:47:25Z
dc.date.available.fl_str_mv 2017-07-13T13:47:25Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PIRES, Renata de Cássia; et al. Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts. PLoS ONE, v.12, n.3, e0173922, 12p, Mar. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20073
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0173922
identifier_str_mv PIRES, Renata de Cássia; et al. Multiplex PCR as a tool for the diagnosis of Leishmania spp. kDNA and the gapdh housekeeping gene of mammal hosts. PLoS ONE, v.12, n.3, e0173922, 12p, Mar. 2017.
1932-6203
10.1371/journal.pone.0173922
url https://www.arca.fiocruz.br/handle/icict/20073
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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