Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7498 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
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Figueira, Cláudio PereiraCroda, Julio Henrique RosaChoy, Henry AHaake, David AReis, Mitermayer Galvão dosKo, Albert IcksangPicardeau, Mathieu2014-04-04T19:21:04Z2014-04-04T19:21:04Z2011FIGUEIRA, C. et al. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology, v. 11, p. 129, 2011.1471-2180https://www.arca.fiocruz.br/handle/icict/7498engPublic Library of ScienceHeterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilVeterans Affairs Greater Los Angeles Health Care System. Division of Infectious Diseases. California, USA / University of California Los Angeles School of Medicine. Department of Medicine. Los Angeles, California, USAVeterans Affairs Greater Los Angeles Health Care System. Division of Infectious Diseases. California, USA / University of California Los Angeles School of Medicine. Department of Medicine. Los Angeles, California, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / University of California Los Angeles School of Medicine. Department of Medicine. Los Angeles, California, USAInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceBACKGROUND: In comparison to other bacterial pathogens, our knowledge of the molecular basis of the pathogenesis of leptospirosis is extremely limited. An improved understanding of leptospiral pathogenetic mechanisms requires reliable tools for functional genetic analysis. Leptospiral immunoglobulin-like (Lig) proteins are surface proteins found in pathogenic Leptospira, but not in saprophytes. Here, we describe a system for heterologous expression of the Leptospira interrogans genes ligA and ligB in the saprophyte Leptospira biflexa serovar Patoc. RESULTS: The genes encoding LigA and LigB under the control of a constitutive spirochaetal promoter were inserted into the L. biflexa replicative plasmid. We were able to demonstrate expression and surface localization of LigA and LigB in L. biflexa. We found that the expression of the lig genes significantly enhanced the ability of transformed L. biflexa to adhere in vitro to extracellular matrix components and cultured cells, suggesting the involvement of Lig proteins in cell adhesion. CONCLUSIONS: This work reports a complete description of the system we have developed for heterologous expression of pathogen-specific proteins in the saprophytic L. biflexa. We show that expression of LigA and LigB proteins from the pathogen confers a virulence-associated phenotype on L. biflexa, namely adhesion to eukaryotic cells and fibronectin in vitro. This study indicates that L. biflexa can serve as a surrogate host to characterize the role of key virulence factors of the causative agent of leptospirosis.Antígenos de Bactérias/metabolismoAderência BacterianaCélulas Epiteliais/microbiologiaFibronectinas/metabolismoLeptospira/patogenicidadeFatores de Virulência/metabolismoAnimaisAntígenos de Bactérias/genéticaLinhagem CelularCãesExpressão GênicaVetores GenéticosLeptospira/genéticaPlasmídeosProteínas Recombinantes/genéticaProteínas Recombinantes/metabolismoFatores de Virulência/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFigieira C. Heterologous.pdfFigieira C. 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dc.title.pt_BR.fl_str_mv |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
title |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
spellingShingle |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. Figueira, Cláudio Pereira Antígenos de Bactérias/metabolismo Aderência Bacteriana Células Epiteliais/microbiologia Fibronectinas/metabolismo Leptospira/patogenicidade Fatores de Virulência/metabolismo Animais Antígenos de Bactérias/genética Linhagem Celular Cães Expressão Gênica Vetores Genéticos Leptospira/genética Plasmídeos Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Fatores de Virulência/genética |
title_short |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
title_full |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
title_fullStr |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
title_full_unstemmed |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
title_sort |
Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. |
author |
Figueira, Cláudio Pereira |
author_facet |
Figueira, Cláudio Pereira Croda, Julio Henrique Rosa Choy, Henry A Haake, David A Reis, Mitermayer Galvão dos Ko, Albert Icksang Picardeau, Mathieu |
author_role |
author |
author2 |
Croda, Julio Henrique Rosa Choy, Henry A Haake, David A Reis, Mitermayer Galvão dos Ko, Albert Icksang Picardeau, Mathieu |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Figueira, Cláudio Pereira Croda, Julio Henrique Rosa Choy, Henry A Haake, David A Reis, Mitermayer Galvão dos Ko, Albert Icksang Picardeau, Mathieu |
dc.subject.decs.pt_BR.fl_str_mv |
Antígenos de Bactérias/metabolismo Aderência Bacteriana Células Epiteliais/microbiologia Fibronectinas/metabolismo Leptospira/patogenicidade Fatores de Virulência/metabolismo Animais Antígenos de Bactérias/genética Linhagem Celular Cães Expressão Gênica Vetores Genéticos Leptospira/genética Plasmídeos Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Fatores de Virulência/genética |
topic |
Antígenos de Bactérias/metabolismo Aderência Bacteriana Células Epiteliais/microbiologia Fibronectinas/metabolismo Leptospira/patogenicidade Fatores de Virulência/metabolismo Animais Antígenos de Bactérias/genética Linhagem Celular Cães Expressão Gênica Vetores Genéticos Leptospira/genética Plasmídeos Proteínas Recombinantes/genética Proteínas Recombinantes/metabolismo Fatores de Virulência/genética |
description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2014-04-04T19:21:04Z |
dc.date.available.fl_str_mv |
2014-04-04T19:21:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FIGUEIRA, C. et al. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology, v. 11, p. 129, 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/7498 |
dc.identifier.issn.none.fl_str_mv |
1471-2180 |
identifier_str_mv |
FIGUEIRA, C. et al. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology, v. 11, p. 129, 2011. 1471-2180 |
url |
https://www.arca.fiocruz.br/handle/icict/7498 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Public Library of Science |
publisher.none.fl_str_mv |
Public Library of Science |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
instacron_str |
FIOCRUZ |
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FIOCRUZ |
reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
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