Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.

Detalhes bibliográficos
Autor(a) principal: Figueira, Cláudio Pereira
Data de Publicação: 2011
Outros Autores: Croda, Julio Henrique Rosa, Choy, Henry A, Haake, David A, Reis, Mitermayer Galvão dos, Ko, Albert Icksang, Picardeau, Mathieu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7498
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Figueira, Cláudio PereiraCroda, Julio Henrique RosaChoy, Henry AHaake, David AReis, Mitermayer Galvão dosKo, Albert IcksangPicardeau, Mathieu2014-04-04T19:21:04Z2014-04-04T19:21:04Z2011FIGUEIRA, C. et al. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology, v. 11, p. 129, 2011.1471-2180https://www.arca.fiocruz.br/handle/icict/7498engPublic Library of ScienceHeterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilVeterans Affairs Greater Los Angeles Health Care System. Division of Infectious Diseases. California, USA / University of California Los Angeles School of Medicine. Department of Medicine. Los Angeles, California, USAVeterans Affairs Greater Los Angeles Health Care System. Division of Infectious Diseases. California, USA / University of California Los Angeles School of Medicine. Department of Medicine. Los Angeles, California, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / University of California Los Angeles School of Medicine. Department of Medicine. Los Angeles, California, USAInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceBACKGROUND: In comparison to other bacterial pathogens, our knowledge of the molecular basis of the pathogenesis of leptospirosis is extremely limited. An improved understanding of leptospiral pathogenetic mechanisms requires reliable tools for functional genetic analysis. Leptospiral immunoglobulin-like (Lig) proteins are surface proteins found in pathogenic Leptospira, but not in saprophytes. Here, we describe a system for heterologous expression of the Leptospira interrogans genes ligA and ligB in the saprophyte Leptospira biflexa serovar Patoc. RESULTS: The genes encoding LigA and LigB under the control of a constitutive spirochaetal promoter were inserted into the L. biflexa replicative plasmid. We were able to demonstrate expression and surface localization of LigA and LigB in L. biflexa. We found that the expression of the lig genes significantly enhanced the ability of transformed L. biflexa to adhere in vitro to extracellular matrix components and cultured cells, suggesting the involvement of Lig proteins in cell adhesion. CONCLUSIONS: This work reports a complete description of the system we have developed for heterologous expression of pathogen-specific proteins in the saprophytic L. biflexa. We show that expression of LigA and LigB proteins from the pathogen confers a virulence-associated phenotype on L. biflexa, namely adhesion to eukaryotic cells and fibronectin in vitro. This study indicates that L. biflexa can serve as a surrogate host to characterize the role of key virulence factors of the causative agent of leptospirosis.Antígenos de Bactérias/metabolismoAderência BacterianaCélulas Epiteliais/microbiologiaFibronectinas/metabolismoLeptospira/patogenicidadeFatores de Virulência/metabolismoAnimaisAntígenos de Bactérias/genéticaLinhagem CelularCãesExpressão GênicaVetores GenéticosLeptospira/genéticaPlasmídeosProteínas Recombinantes/genéticaProteínas Recombinantes/metabolismoFatores de Virulência/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFigieira C. Heterologous.pdfFigieira C. 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dc.title.pt_BR.fl_str_mv Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
title Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
spellingShingle Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
Figueira, Cláudio Pereira
Antígenos de Bactérias/metabolismo
Aderência Bacteriana
Células Epiteliais/microbiologia
Fibronectinas/metabolismo
Leptospira/patogenicidade
Fatores de Virulência/metabolismo
Animais
Antígenos de Bactérias/genética
Linhagem Celular
Cães
Expressão Gênica
Vetores Genéticos
Leptospira/genética
Plasmídeos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Fatores de Virulência/genética
title_short Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
title_full Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
title_fullStr Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
title_full_unstemmed Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
title_sort Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin.
author Figueira, Cláudio Pereira
author_facet Figueira, Cláudio Pereira
Croda, Julio Henrique Rosa
Choy, Henry A
Haake, David A
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Picardeau, Mathieu
author_role author
author2 Croda, Julio Henrique Rosa
Choy, Henry A
Haake, David A
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Picardeau, Mathieu
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figueira, Cláudio Pereira
Croda, Julio Henrique Rosa
Choy, Henry A
Haake, David A
Reis, Mitermayer Galvão dos
Ko, Albert Icksang
Picardeau, Mathieu
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Bactérias/metabolismo
Aderência Bacteriana
Células Epiteliais/microbiologia
Fibronectinas/metabolismo
Leptospira/patogenicidade
Fatores de Virulência/metabolismo
Animais
Antígenos de Bactérias/genética
Linhagem Celular
Cães
Expressão Gênica
Vetores Genéticos
Leptospira/genética
Plasmídeos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Fatores de Virulência/genética
topic Antígenos de Bactérias/metabolismo
Aderência Bacteriana
Células Epiteliais/microbiologia
Fibronectinas/metabolismo
Leptospira/patogenicidade
Fatores de Virulência/metabolismo
Animais
Antígenos de Bactérias/genética
Linhagem Celular
Cães
Expressão Gênica
Vetores Genéticos
Leptospira/genética
Plasmídeos
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Fatores de Virulência/genética
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-04-04T19:21:04Z
dc.date.available.fl_str_mv 2014-04-04T19:21:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FIGUEIRA, C. et al. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology, v. 11, p. 129, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7498
dc.identifier.issn.none.fl_str_mv 1471-2180
identifier_str_mv FIGUEIRA, C. et al. Heterologous expression of pathogen-specific genes ligA and ligB in the saprophyte Leptospira biflexa confers enhanced adhesion to cultured cells and fibronectin. BMC Microbiology, v. 11, p. 129, 2011.
1471-2180
url https://www.arca.fiocruz.br/handle/icict/7498
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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