Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Oliveira, Camila
Data de Publicação: 2021
Outros Autores: Gerber, André P., Goldenberg, Samuel, Alves, Lysangela Ronalte
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.3390/microorganisms9050986
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47650
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil / Axe Neurosciences. Département de Pédiatrie. Faculté de Médecine. Université Laval. Quebec City, Canada.
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spelling Oliveira, CamilaGerber, André P.Goldenberg, SamuelAlves, Lysangela Ronalte2021-06-11T16:01:16Z2021-06-11T16:01:16Z2021OLIVEIRA, Camila et al. Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi. Microorganisms, v. 9, n. 986, p. 1-12, 2021.2076-2607https://www.arca.fiocruz.br/handle/icict/4765010.3390/microorganisms9050986porMDPITrypanosoma cruziTcSgn1Gene Expression RegulationRNA-Binding ProteinsRegulación de la Expresión GénicaProteínas de Unión al ARNRégulation de l'expression des gènesProtéines de liaison à l'ARNRegulação da Expressão GênicaProteínas de Ligação a RNASaccharomyces cerevisiaeCharacterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil / Axe Neurosciences. Département de Pédiatrie. Faculté de Médecine. Université Laval. Quebec City, Canada.Department of Microbial Sciences. Faculty of Health and Medical Sciences. University of Surrey. Guildford, Surrey, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.RNA-binding proteins (RBPs) participate in several steps of post-transcriptional regulation of gene expression, such as splicing, messenger RNA transport, mRNA localization, and translation. Gene-expression regulation in trypanosomatids occurs primarily at the post-transcriptional level, and RBPs play important roles in the process. Here, we characterized the RBP TcSgn1, which contains one RNA recognition motif (RRM). TcSgn1 is a close ortholog of yeast Saccharomyces cerevisiae protein ScSgn1, which plays a role in translational regulation in the cytoplasm. We found that TcSgn1 in Trypanosoma cruzi is localized in the nucleus in exponentially growing epimastigotes. By performing immunoprecipitation assays of TcSgn1, we identified hundreds of mRNAs associated with the protein, a significant fraction of them coding for nucleic acids binding, transcription, and endocytosis proteins. In addition, we show that TcSgn1 is capable of interacting directly with the poly(A) tail of the mRNAs. The study of parasites under nutritional stress showed that TcSgn1 was localized in cytoplasmic granules in addition to localizing in the nucleus. Similar to ScSgn1, we observed that TcSgn1 also interacts with the PABP1 protein, suggesting that this protein may play a role in regulating gene expression in T. cruzi. Taken together, our results show that RNA-binding protein TcSgn1 is part of ribonucleoprotein complexes associated with nuclear functions, stress response, and RNA metabolism.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/47650/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmicroorganisms-09-0098OK.pdfmicroorganisms-09-0098OK.pdfapplication/pdf9894496https://www.arca.fiocruz.br/bitstream/icict/47650/2/microorganisms-09-0098OK.pdf2c579ac81db5b5c082a65f55d023f76bMD52TEXTmicroorganisms-09-0098OK.pdf.txtmicroorganisms-09-0098OK.pdf.txtExtracted texttext/plain39233https://www.arca.fiocruz.br/bitstream/icict/47650/3/microorganisms-09-0098OK.pdf.txt3c6315e23ad005f74cade972581d22afMD53icict/476502021-06-14 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dc.title.pt_BR.fl_str_mv Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
title Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
spellingShingle Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
Oliveira, Camila
Trypanosoma cruzi
TcSgn1
Gene Expression Regulation
RNA-Binding Proteins
Regulación de la Expresión Génica
Proteínas de Unión al ARN
Régulation de l'expression des gènes
Protéines de liaison à l'ARN
Regulação da Expressão Gênica
Proteínas de Ligação a RNA
Saccharomyces cerevisiae
title_short Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
title_full Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
title_fullStr Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
title_full_unstemmed Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
title_sort Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi
author Oliveira, Camila
author_facet Oliveira, Camila
Gerber, André P.
Goldenberg, Samuel
Alves, Lysangela Ronalte
author_role author
author2 Gerber, André P.
Goldenberg, Samuel
Alves, Lysangela Ronalte
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Camila
Gerber, André P.
Goldenberg, Samuel
Alves, Lysangela Ronalte
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
TcSgn1
topic Trypanosoma cruzi
TcSgn1
Gene Expression Regulation
RNA-Binding Proteins
Regulación de la Expresión Génica
Proteínas de Unión al ARN
Régulation de l'expression des gènes
Protéines de liaison à l'ARN
Regulação da Expressão Gênica
Proteínas de Ligação a RNA
Saccharomyces cerevisiae
dc.subject.en.pt_BR.fl_str_mv Gene Expression Regulation
RNA-Binding Proteins
dc.subject.es.pt_BR.fl_str_mv Regulación de la Expresión Génica
Proteínas de Unión al ARN
dc.subject.fr.pt_BR.fl_str_mv Régulation de l'expression des gènes
Protéines de liaison à l'ARN
dc.subject.decs.pt_BR.fl_str_mv Regulação da Expressão Gênica
Proteínas de Ligação a RNA
Saccharomyces cerevisiae
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil / Axe Neurosciences. Département de Pédiatrie. Faculté de Médecine. Université Laval. Quebec City, Canada.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-06-11T16:01:16Z
dc.date.available.fl_str_mv 2021-06-11T16:01:16Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Camila et al. Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi. Microorganisms, v. 9, n. 986, p. 1-12, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47650
dc.identifier.issn.pt_BR.fl_str_mv 2076-2607
dc.identifier.doi.none.fl_str_mv 10.3390/microorganisms9050986
identifier_str_mv OLIVEIRA, Camila et al. Characterization of the RNA-Binding Protein TcSgn1 in Trypanosoma cruzi. Microorganisms, v. 9, n. 986, p. 1-12, 2021.
2076-2607
10.3390/microorganisms9050986
url https://www.arca.fiocruz.br/handle/icict/47650
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/47650/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/47650/2/microorganisms-09-0098OK.pdf
https://www.arca.fiocruz.br/bitstream/icict/47650/3/microorganisms-09-0098OK.pdf.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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