Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection

Detalhes bibliográficos
Autor(a) principal: Paulino, Patrícia
Data de Publicação: 2021
Outros Autores: Vitari, Gabriela, Rezende, Antonio, Couto, Joana, Antunes, Sandra, Domingos, Ana, Peckle, Maristela, Massard, Carlos, Araújo, Flávio Marcos Gomes de, Santos, Huarrisson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51177
Resumo: Department of Epidemiology and Public Health. Federal Rural University of Rio de Janeiro. Seropedica, RJ, Brazil
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spelling Paulino, PatríciaVitari, GabrielaRezende, AntonioCouto, JoanaAntunes, SandraDomingos, AnaPeckle, MaristelaMassard, CarlosAraújo, Flávio Marcos Gomes deSantos, Huarrisson2022-02-14T16:16:38Z2022-02-14T16:16:38Z2021PAULINO, Patrícia et al. Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection. Pathogens, V. 10, 167, 2021. https://doi.org/10.3390/pathogens100201672076-0817https://www.arca.fiocruz.br/handle/icict/51177engMDPICharacterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Epidemiology and Public Health. Federal Rural University of Rio de Janeiro. Seropedica, RJ, BrazilDepartment of Epidemiology and Public Health. Federal Rural University of Rio de Janeiro. Seropedica, RJ, BrazilDepartment of Microbiology. Institute Aggeu Magalhães. Oswaldo Cruz Foundation. Recife, BrazilGlobal Health and Tropical Medicine. Instituto de Higiene e Medicina Tropical. Universidade Nova de Lisboa. Lisbon, PortugalGlobal Health and Tropical Medicine. Instituto de Higiene e Medicina Tropical. Universidade Nova de Lisboa. Lisbon, PortugalGlobal Health and Tropical Medicine. Instituto de Higiene e Medicina Tropical. Universidade Nova de Lisboa. Lisbon, PortugalDepartment of Animal Parasitology. Federal Rural University of Rio de Janeiro. Seropedica, RJ, BrazilDepartment of Animal Parasitology. Federal Rural University of Rio de Janeiro. Seropedica, RJ, BrazilRene Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, BrazilDepartment of Epidemiology and Public Health. Federal Rural University of Rio de Janeiro. Seropedica, RJ, BrazilThis study intends to characterize the sialotranscriptome profile of Rhipicephalus (Boophilus) microplus in response to Theileria equi and identify genes of interest with differential genomic expression, indicating relevant targets in the tick–protozoan interactions. The experimental design consisted of RNA sequencing from uninfected and T. equi-infected R. microplus salivary glands (SGs) to obtain transcriptomic profiles for characterization and comparison. A total of 288,952 transcripts were obtained from both tick profiles, 3456 transcripts (p < 0.05) differentially expressed in response to T. equi infection. The uninfected SGs’ registered 231,179 transcripts, of which 155,359 were annotated. The most transcribed sequences were female-specific histamine binding protein and lipocalins. Regarding the T. equi-infected SGs, from the 238,964 assembled transcripts, 163,564 were annotated. The most transcribed sequences were histone demethylase JARID1 and Y-box-binding protein. Five transcripts (cystatin, arginase, nuclear factor κB kinase inhibitor subunit β (IκB), IκB delta, lysosomal-trafficking regulator, and reeler protein) presented the gene ontology (GO) category “response to protozoan” and were exclusively displayed in the T. equi-infected profile. The transcriptome of T. equi was also analyzed, registering 4728 hits. The study’s genetic and molecular information would be of great value for future studies and biotechnological applications envisaging disease control.equine piroplasmosisRNA-seqtick defensetick-protozoan interactionsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51177/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALCharacterization of the Rhipicephalus (Boophilus) .pdfCharacterization of the Rhipicephalus (Boophilus) .pdfapplication/pdf9518407https://www.arca.fiocruz.br/bitstream/icict/51177/2/Characterization%20of%20the%20Rhipicephalus%20%28Boophilus%29%20.pdfc64284d74081717924194a00e8956885MD52icict/511772022-02-14 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dc.title.pt_BR.fl_str_mv Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
title Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
spellingShingle Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
Paulino, Patrícia
equine piroplasmosis
RNA-seq
tick defense
tick-protozoan interactions
title_short Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
title_full Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
title_fullStr Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
title_full_unstemmed Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
title_sort Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection
author Paulino, Patrícia
author_facet Paulino, Patrícia
Vitari, Gabriela
Rezende, Antonio
Couto, Joana
Antunes, Sandra
Domingos, Ana
Peckle, Maristela
Massard, Carlos
Araújo, Flávio Marcos Gomes de
Santos, Huarrisson
author_role author
author2 Vitari, Gabriela
Rezende, Antonio
Couto, Joana
Antunes, Sandra
Domingos, Ana
Peckle, Maristela
Massard, Carlos
Araújo, Flávio Marcos Gomes de
Santos, Huarrisson
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paulino, Patrícia
Vitari, Gabriela
Rezende, Antonio
Couto, Joana
Antunes, Sandra
Domingos, Ana
Peckle, Maristela
Massard, Carlos
Araújo, Flávio Marcos Gomes de
Santos, Huarrisson
dc.subject.en.pt_BR.fl_str_mv equine piroplasmosis
RNA-seq
tick defense
tick-protozoan interactions
topic equine piroplasmosis
RNA-seq
tick defense
tick-protozoan interactions
description Department of Epidemiology and Public Health. Federal Rural University of Rio de Janeiro. Seropedica, RJ, Brazil
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-02-14T16:16:38Z
dc.date.available.fl_str_mv 2022-02-14T16:16:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAULINO, Patrícia et al. Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection. Pathogens, V. 10, 167, 2021. https://doi.org/10.3390/pathogens10020167
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51177
dc.identifier.issn.pt_BR.fl_str_mv 2076-0817
identifier_str_mv PAULINO, Patrícia et al. Characterization of the Rhipicephalus (Boophilus) microplus Sialotranscriptome Profile in Response to Theileria equi Infection. Pathogens, V. 10, 167, 2021. https://doi.org/10.3390/pathogens10020167
2076-0817
url https://www.arca.fiocruz.br/handle/icict/51177
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51177/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51177/2/Characterization%20of%20the%20Rhipicephalus%20%28Boophilus%29%20.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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