First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19009 |
Resumo: | National Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands. |
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de Beer, Jessica L.Kremer, KristinKödmön, CsabaSupply, Philipvan Soolingen, Dick2017-05-25T16:29:55Z2017-05-25T16:29:55Z2012de Beer, Jessica L. et al. First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains. Journal of Clinical Microbiology, v.50, n.3, p.662-669, Mar. 2012.0095-1137https://www.arca.fiocruz.br/handle/icict/1900910.1128/JCM.00607-111098-660XengAmerican Society for MicrobiologyMycobacterium tuberculosisEstudo mundial de ProficiênciaVariáveis tipagensMycobacterium tuberculosisProficiency StudyVariable-Number Tandem-Repeat TypingFirst worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands.World Health Organization. Regional Office for Europe. Health Security & Environment. Division of Communicable Diseases. Tuberculosis and M/XDR-TB Programme. Copenhagen, Denmark..European Centre for Disease Prevention and Control. Tuberculosis Programme. Surveillance Unit. Stockholm, Sweden.Université Lille Nord de France and Institut Pasteur de Lille. Center for Infection and Immunity of Lille, INSERM U1019-CNRS UMR 8204.. Lille, France / GenoScreen. Lille, France.National Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands / Radboud University Nijmegen Medical Centre, Dekkerswald. Medical Microbiology. Nijmegen, Netherlands.Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19009/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALphilip_suffys_etal_IOC_2012.pdfphilip_suffys_etal_IOC_2012.pdfapplication/pdf320574https://www.arca.fiocruz.br/bitstream/icict/19009/2/philip_suffys_etal_IOC_2012.pdf4a7dd2dda5fd74c2c53454f832284a49MD52TEXTphilip_suffys_etal_IOC_2012.pdf.txtphilip_suffys_etal_IOC_2012.pdf.txtExtracted texttext/plain48606https://www.arca.fiocruz.br/bitstream/icict/19009/3/philip_suffys_etal_IOC_2012.pdf.txt294fec9ac76de6b1dceae7628dda24d8MD53icict/190092018-08-15 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dc.title.pt_BR.fl_str_mv |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
title |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
spellingShingle |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains de Beer, Jessica L. Mycobacterium tuberculosis Estudo mundial de Proficiência Variáveis tipagens Mycobacterium tuberculosis Proficiency Study Variable-Number Tandem-Repeat Typing |
title_short |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
title_full |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
title_fullStr |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
title_full_unstemmed |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
title_sort |
First worldwide proficiency study on variable-number tandem-repeat typing of Mycobacterium tuberculosis complex strains |
author |
de Beer, Jessica L. |
author_facet |
de Beer, Jessica L. Kremer, Kristin Ködmön, Csaba Supply, Philip van Soolingen, Dick |
author_role |
author |
author2 |
Kremer, Kristin Ködmön, Csaba Supply, Philip van Soolingen, Dick |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
de Beer, Jessica L. Kremer, Kristin Ködmön, Csaba Supply, Philip van Soolingen, Dick |
dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Estudo mundial de Proficiência Variáveis tipagens |
topic |
Mycobacterium tuberculosis Estudo mundial de Proficiência Variáveis tipagens Mycobacterium tuberculosis Proficiency Study Variable-Number Tandem-Repeat Typing |
dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium tuberculosis Proficiency Study Variable-Number Tandem-Repeat Typing |
description |
National Institute for Public Health and the Environment. Centre for Infectious Disease Control. National Tuberculosis Reference Laboratory. Laboratory for Infectious Diseases and Perinatal Screening. Bilthoven, Netherlands. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2017-05-25T16:29:55Z |
dc.date.available.fl_str_mv |
2017-05-25T16:29:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
de Beer, Jessica L. et al. First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains. Journal of Clinical Microbiology, v.50, n.3, p.662-669, Mar. 2012. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/19009 |
dc.identifier.issn.pt_BR.fl_str_mv |
0095-1137 |
dc.identifier.doi.none.fl_str_mv |
10.1128/JCM.00607-11 |
dc.identifier.eissn.none.fl_str_mv |
1098-660X |
identifier_str_mv |
de Beer, Jessica L. et al. First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains. Journal of Clinical Microbiology, v.50, n.3, p.662-669, Mar. 2012. 0095-1137 10.1128/JCM.00607-11 1098-660X |
url |
https://www.arca.fiocruz.br/handle/icict/19009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Society for Microbiology |
publisher.none.fl_str_mv |
American Society for Microbiology |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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