Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.

Detalhes bibliográficos
Autor(a) principal: Athanazio, Daniel Abensur
Data de Publicação: 2008
Outros Autores: Silva, Éverton Fagonde, Santos, Cleiton Silva, Rocha, Gustavo M, Santos, Marcos André Vannier dos, McBride, Alan John Alexander, Ko, Albert Icksang, Reis, Mitermayer Galvão dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7523
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brasil
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spelling Athanazio, Daniel AbensurSilva, Éverton FagondeSantos, Cleiton SilvaRocha, Gustavo MSantos, Marcos André Vannier dosMcBride, Alan John AlexanderKo, Albert IcksangReis, Mitermayer Galvão dos2014-04-15T16:36:31Z2014-04-15T16:36:31Z2008ATHANAZIO, D. A. et al. Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans. Acta Tropica, v. 105, n. 2, p. 176-180, 2008.0001-706X10.1016/j.actatropica.2007.10.012https://www.arca.fiocruz.br/handle/icict/7523engElsevier B.VRattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Pelotas. Biotechnology Center. Rio Grande do Sul, BrazilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Division of International Medicine and Infectious Disease. Weill Medical College of Cornell University. NY, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, BrasilLeptospirosis continues to be a disease with a poorly understood pathogenesis. The experimental rat model is amenable for the investigation of leptospiral dissemination, tropism, persistence of renal colonization and factors related to disease resistance. In this study, Wistar rats were infected intraperitoneally with virulent Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130. The detection of leptospires in tissue samples was based on culture, silver staining and immunofluorescence techniques. An inoculum of 10,000 leptospires induced colonization in 50% of rats and colonization persisted for the 4-month period of the study. Dissemination kinetics revealed that renal colonization took place 7-9 days after infection, with no underlying histopathology. The peak leptospiral load occurred on day 5 post-infection, followed by rapid clearance in all tissues except the kidneys, where dense leptospiral aggregates persisted in the renal tubules. We conclude that the experimental rat model is suitable for studies contributing towards the understanding of the mechanisms of colonization and resistance to severe disease in leptospirosisLeptospirosisLeptospiraRatsAnimal disease modelsModelos Animais de DoençasRim/patologiaLeptospira interrogans/patogenicidadeLeptospirose/patologiaAnimaisFemininoRim/microbiologiaLeptospirose/microbiologiaMicroscopia Eletrônica de VarreduraRatosRatos WistarVirulênciainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAthanazio DA Rattus norvegicus as a model....pdfAthanazio DA Rattus norvegicus as a model....pdfapplication/pdf519054https://www.arca.fiocruz.br/bitstream/icict/7523/1/Athanazio%20DA%20Rattus%20norvegicus%20as%20a%20model....pdfb26212d7cd8c6103f0dc80e619674014MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7523/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTAthanazio DA Rattus norvegicus as a model....pdf.txtAthanazio DA Rattus norvegicus as a model....pdf.txtExtracted texttext/plain26889https://www.arca.fiocruz.br/bitstream/icict/7523/5/Athanazio%20DA%20Rattus%20norvegicus%20as%20a%20model....pdf.txt740a525c642063ad38b92e0b5d509886MD55THUMBNAILAthanazio DA Rattus norvegicus as a model....pdf.jpgAthanazio DA Rattus norvegicus as a model....pdf.jpgGenerated Thumbnailimage/jpeg2252https://www.arca.fiocruz.br/bitstream/icict/7523/4/Athanazio%20DA%20Rattus%20norvegicus%20as%20a%20model....pdf.jpg607a6f169789d11f3da0e855153b9591MD54icict/75232023-03-15 14:33:05.582oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
title Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
spellingShingle Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
Athanazio, Daniel Abensur
Leptospirosis
Leptospira
Rats
Animal disease models
Modelos Animais de Doenças
Rim/patologia
Leptospira interrogans/patogenicidade
Leptospirose/patologia
Animais
Feminino
Rim/microbiologia
Leptospirose/microbiologia
Microscopia Eletrônica de Varredura
Ratos
Ratos Wistar
Virulência
title_short Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
title_full Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
title_fullStr Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
title_full_unstemmed Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
title_sort Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans.
author Athanazio, Daniel Abensur
author_facet Athanazio, Daniel Abensur
Silva, Éverton Fagonde
Santos, Cleiton Silva
Rocha, Gustavo M
Santos, Marcos André Vannier dos
McBride, Alan John Alexander
Ko, Albert Icksang
Reis, Mitermayer Galvão dos
author_role author
author2 Silva, Éverton Fagonde
Santos, Cleiton Silva
Rocha, Gustavo M
Santos, Marcos André Vannier dos
McBride, Alan John Alexander
Ko, Albert Icksang
Reis, Mitermayer Galvão dos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Athanazio, Daniel Abensur
Silva, Éverton Fagonde
Santos, Cleiton Silva
Rocha, Gustavo M
Santos, Marcos André Vannier dos
McBride, Alan John Alexander
Ko, Albert Icksang
Reis, Mitermayer Galvão dos
dc.subject.en.pt_BR.fl_str_mv Leptospirosis
Leptospira
Rats
Animal disease models
topic Leptospirosis
Leptospira
Rats
Animal disease models
Modelos Animais de Doenças
Rim/patologia
Leptospira interrogans/patogenicidade
Leptospirose/patologia
Animais
Feminino
Rim/microbiologia
Leptospirose/microbiologia
Microscopia Eletrônica de Varredura
Ratos
Ratos Wistar
Virulência
dc.subject.decs.pt_BR.fl_str_mv Modelos Animais de Doenças
Rim/patologia
Leptospira interrogans/patogenicidade
Leptospirose/patologia
Animais
Feminino
Rim/microbiologia
Leptospirose/microbiologia
Microscopia Eletrônica de Varredura
Ratos
Ratos Wistar
Virulência
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-04-15T16:36:31Z
dc.date.available.fl_str_mv 2014-04-15T16:36:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ATHANAZIO, D. A. et al. Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans. Acta Tropica, v. 105, n. 2, p. 176-180, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7523
dc.identifier.issn.none.fl_str_mv 0001-706X
10.1016/j.actatropica.2007.10.012
identifier_str_mv ATHANAZIO, D. A. et al. Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans. Acta Tropica, v. 105, n. 2, p. 176-180, 2008.
0001-706X
10.1016/j.actatropica.2007.10.012
url https://www.arca.fiocruz.br/handle/icict/7523
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier B.V
publisher.none.fl_str_mv Elsevier B.V
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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