Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.

Detalhes bibliográficos
Autor(a) principal: Vinhas, Vera
Data de Publicação: 2007
Outros Autores: Andrade, Bruno de Bezerril, Paes, Fábio, Bomura, Andréa, Clarêncio, Jorge, Miranda, José Carlos, Bafica, Andre Luiz Barbosa, Barral, Aldina Maria Prado, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7412
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
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spelling Vinhas, VeraAndrade, Bruno de BezerrilPaes, FábioBomura, AndréaClarêncio, JorgeMiranda, José CarlosBafica, Andre Luiz BarbosaBarral, Aldina Maria PradoBarral Netto, Manoel2014-03-17T17:40:44Z2014-03-17T17:40:44Z2007VINHAS, V. et al. Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis. European Journal of Immunology, v. 37, p. 3111–3121, 2007.0014-2980https://www.arca.fiocruz.br/handle/icict/7412engWILEY-VCH Verlag GmbH & Co.Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilNational Institutes of Health NIAID. National Institutes of Health. Immunobiology Section. Bethesda, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. / Salvador, BA, Brasil / Instituto de Investiga ção em Imunologia. Instituto do Milênio. SAlvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. / Salvador, BA, Brasil / Instituto de Investiga ção em Imunologia. Instituto do Milênio. SAlvador, BA, BrasilExperiments in animals verified that phlebotomine saliva enhances Leishmania infection, and vaccination with saliva prevents disease. We have shown that individuals from an endemic area of visceral leishmaniasis displayed robust antibody responses to saliva from the vector Lutzomyia longipalpis, which correlated with anti-parasite cellmediated immunity. Here, we explored human anti-saliva responses following exposure to sand flies, using an in vivo bite model in which normal volunteers were exposed four times to 30 laboratory-reared Lu. longipalpis. Following the third exposure, normal volunteers developed diverse dermatological reactions at the site of insect bite. Serum from normal volunteers displayed high levels of anti-salivary gland sonicate IgG1, IgG4 and IgE as well as several salivary gland proteins. Furthermore, following in vitro stimulation with salivary gland sonicate, there was an increased frequency of CD4+CD25+ and CD8+CD25+ T cells as well as IFN-c and IL-10 synthesis. Strikingly, 1 year after the first exposure, PBMC from the volunteers displayed recall IFN-c responses that correlated with a significant reduction in infection rates using a macrophage-lymphocyte autologous culture. Together, these data suggest that human immunization against sand fly saliva is feasible and recall responses are obtained even 1 year after exposure, opening perspectives for vaccination in man.Proteínas de Insetos/imunologiaInsetos Vetores/imunologiaLeishmaniose Visceral/prevenção & controlePsychodidae/imunologiaSaliva/imunologiaAdultoAnimaisWestern BlottingCitocinas/sangueCitocinas/imunologiaEnsaio de Imunoadsorção EnzimáticaFemininoCitometria de FluxoHumanosImunoglobulina E/sangueImunoglobulina G/sangueMemória ImunológicaLeishmaniose Visceral/transmissãoMasculinoProteínas e Peptídeos Salivares/imunologiaLinfócitos T/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALVinhas V Human anti-saliva....pdfVinhas V Human anti-saliva....pdfapplication/pdf347167https://www.arca.fiocruz.br/bitstream/icict/7412/1/Vinhas%20V%20Human%20anti-saliva....pdf54a24ccf2c3aab33b0ff6aebd5613dc7MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7412/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTVinhas V Human anti-saliva....pdf.txtVinhas V Human anti-saliva....pdf.txtExtracted texttext/plain44810https://www.arca.fiocruz.br/bitstream/icict/7412/5/Vinhas%20V%20Human%20anti-saliva....pdf.txt5e89aee34fee81ccfe51174dc5c1ae24MD55THUMBNAILVinhas V Human anti-saliva....pdf.jpgVinhas V Human anti-saliva....pdf.jpgGenerated Thumbnailimage/jpeg2185https://www.arca.fiocruz.br/bitstream/icict/7412/4/Vinhas%20V%20Human%20anti-saliva....pdf.jpg980a80ce8b2df3836a9c4aa5671c3f16MD54icict/74122023-03-15 14:33:05.683oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
title Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
spellingShingle Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
Vinhas, Vera
Proteínas de Insetos/imunologia
Insetos Vetores/imunologia
Leishmaniose Visceral/prevenção & controle
Psychodidae/imunologia
Saliva/imunologia
Adulto
Animais
Western Blotting
Citocinas/sangue
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Humanos
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Memória Imunológica
Leishmaniose Visceral/transmissão
Masculino
Proteínas e Peptídeos Salivares/imunologia
Linfócitos T/imunologia
title_short Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
title_full Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
title_fullStr Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
title_full_unstemmed Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
title_sort Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis.
author Vinhas, Vera
author_facet Vinhas, Vera
Andrade, Bruno de Bezerril
Paes, Fábio
Bomura, Andréa
Clarêncio, Jorge
Miranda, José Carlos
Bafica, Andre Luiz Barbosa
Barral, Aldina Maria Prado
Barral Netto, Manoel
author_role author
author2 Andrade, Bruno de Bezerril
Paes, Fábio
Bomura, Andréa
Clarêncio, Jorge
Miranda, José Carlos
Bafica, Andre Luiz Barbosa
Barral, Aldina Maria Prado
Barral Netto, Manoel
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vinhas, Vera
Andrade, Bruno de Bezerril
Paes, Fábio
Bomura, Andréa
Clarêncio, Jorge
Miranda, José Carlos
Bafica, Andre Luiz Barbosa
Barral, Aldina Maria Prado
Barral Netto, Manoel
dc.subject.decs.pt_BR.fl_str_mv Proteínas de Insetos/imunologia
Insetos Vetores/imunologia
Leishmaniose Visceral/prevenção & controle
Psychodidae/imunologia
Saliva/imunologia
Adulto
Animais
Western Blotting
Citocinas/sangue
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Humanos
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Memória Imunológica
Leishmaniose Visceral/transmissão
Masculino
Proteínas e Peptídeos Salivares/imunologia
Linfócitos T/imunologia
topic Proteínas de Insetos/imunologia
Insetos Vetores/imunologia
Leishmaniose Visceral/prevenção & controle
Psychodidae/imunologia
Saliva/imunologia
Adulto
Animais
Western Blotting
Citocinas/sangue
Citocinas/imunologia
Ensaio de Imunoadsorção Enzimática
Feminino
Citometria de Fluxo
Humanos
Imunoglobulina E/sangue
Imunoglobulina G/sangue
Memória Imunológica
Leishmaniose Visceral/transmissão
Masculino
Proteínas e Peptídeos Salivares/imunologia
Linfócitos T/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-03-17T17:40:44Z
dc.date.available.fl_str_mv 2014-03-17T17:40:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VINHAS, V. et al. Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis. European Journal of Immunology, v. 37, p. 3111–3121, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7412
dc.identifier.issn.none.fl_str_mv 0014-2980
identifier_str_mv VINHAS, V. et al. Human anti-saliva immune response following experimental exposure to the visceral leishmaniasis vector, Lutzomyia longipalpis. European Journal of Immunology, v. 37, p. 3111–3121, 2007.
0014-2980
url https://www.arca.fiocruz.br/handle/icict/7412
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv WILEY-VCH Verlag GmbH & Co.
publisher.none.fl_str_mv WILEY-VCH Verlag GmbH & Co.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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