A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis

Detalhes bibliográficos
Autor(a) principal: Melo, Stella Maria Barrouin
Data de Publicação: 2006
Outros Autores: Larangeira, Daniela Farias, Santos, Silvana Ornelas, Chagas Júnior, Adenizar Delgado das, Paixão, Mariza S, Aguiar, Paulo Henrique Palis, dosSantos, Washington Luis Conrado, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/2742
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Melo, Stella Maria BarrouinLarangeira, Daniela FariasSantos, Silvana OrnelasChagas Júnior, Adenizar Delgado dasPaixão, Mariza SAguiar, Paulo Henrique PalisdosSantos, Washington Luis ConradoPontes-de-Carvalho, Lain Carlos2011-07-01T19:16:02Z2011-07-01T19:16:02Z2006BARROUIN-MELO, S. M. et al. A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis. Veterinary Immunology and Immunopathology, v. 111, n. 3-4, p. 251-261, 2006.0165-2427https://www.arca.fiocruz.br/handle/icict/2742engBaçoCaninoBiópsia Aspirativa com Agulha FinaPopulações de LeucócitosLeishmaniose Visceral CaninaSpleenCanineFine-needle Aspirative BiopsyLeukocyte PopulationsCanine Visceral LeishmaniosisBaçoBiópsia por AgulhaLeucócitosLeishmaniose VisceralA standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Departamento de Patologia e Clínicas. Escola de Medicina Veterinária. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, BrasilLaboratório Central do Estado da Bahia, Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Departamento de Patologia e Clínicas. Escola de Medicina Veterinária. Salvador, BA, BrasilUniversidade Federal da Bahia. Escola de Medicina Veterinária. Laboratório de Infectologia Veterinária. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação para o Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilA method for the evaluation of splenic cellularity using samples collected by fine-needle aspirative biopsy was standardized in this work. The procedure includes erythrocyte lysing, preparation of cytospin films and staining by histochemical and immunocytochemical techniques. The cellular profiles of spleen preparations were compared with those observed in peripheral blood samples subjected to the same procedure. Two groups were compared, one consisting of 14 healthy uninfected and the other of 15 polysymptomatic Leishmania chagasi/infantum-infected dogs, from an endemic area for visceral leishmaniosis. Cell populations were identified by conventional hematoxilin–eosin and Wright’ stainings, and by immunocytochemistry using monoclonal antibodies against canine CD45RA and CD45RB, phagocytes and a pan-leukocyte antigen. Larger neutrophil (P < 0.0001) and monocyte/macrophage (P = 0.0036) relative counts and lower lymphocyte relative counts (P < 0.0001) were found in the spleen, and not in the blood, of the animals with leishmaniosis than in those of the healthy animals. The proportions of CD45RB+ cells were higher, and of CD45RA+ cells were lower, both in the spleen and in the blood of animals with leishmaniosis than in those of healthy dogs (P < 0.05). Additionally, hematoxilin–eosin-stained cytospins of spleen aspirates from Leishmania-infected animals permitted the easy visualization of amastigote forms inside phagocytes, under light microscopy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBarrouin-Melo_Larangeira_Santos_etal.pdfBarrouin-Melo_Larangeira_Santos_etal.pdfapplication/pdf337692https://www.arca.fiocruz.br/bitstream/icict/2742/1/Barrouin-Melo_Larangeira_Santos_etal.pdf085d3627daac4f4f547fcda56dfbede8MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/2742/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTBarrouin-Melo_Larangeira_Santos_etal.pdf.txtBarrouin-Melo_Larangeira_Santos_etal.pdf.txtExtracted texttext/plain43647https://www.arca.fiocruz.br/bitstream/icict/2742/5/Barrouin-Melo_Larangeira_Santos_etal.pdf.txt95694eabb5e3b673c60fa1c23f4f21b3MD55THUMBNAILBarrouin-Melo_Larangeira_Santos_etal.pdf.jpgBarrouin-Melo_Larangeira_Santos_etal.pdf.jpgGenerated Thumbnailimage/jpeg1870https://www.arca.fiocruz.br/bitstream/icict/2742/4/Barrouin-Melo_Larangeira_Santos_etal.pdf.jpg954911460b66de8b7290e927cee5a8b7MD54icict/27422023-03-15 14:32:59.662oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:59Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
title A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
spellingShingle A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
Melo, Stella Maria Barrouin
Baço
Canino
Biópsia Aspirativa com Agulha Fina
Populações de Leucócitos
Leishmaniose Visceral Canina
Spleen
Canine
Fine-needle Aspirative Biopsy
Leukocyte Populations
Canine Visceral Leishmaniosis
Baço
Biópsia por Agulha
Leucócitos
Leishmaniose Visceral
title_short A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
title_full A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
title_fullStr A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
title_full_unstemmed A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
title_sort A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis
author Melo, Stella Maria Barrouin
author_facet Melo, Stella Maria Barrouin
Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza S
Aguiar, Paulo Henrique Palis
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza S
Aguiar, Paulo Henrique Palis
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo, Stella Maria Barrouin
Larangeira, Daniela Farias
Santos, Silvana Ornelas
Chagas Júnior, Adenizar Delgado das
Paixão, Mariza S
Aguiar, Paulo Henrique Palis
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
dc.subject.other.pt_BR.fl_str_mv Baço
Canino
Biópsia Aspirativa com Agulha Fina
Populações de Leucócitos
Leishmaniose Visceral Canina
topic Baço
Canino
Biópsia Aspirativa com Agulha Fina
Populações de Leucócitos
Leishmaniose Visceral Canina
Spleen
Canine
Fine-needle Aspirative Biopsy
Leukocyte Populations
Canine Visceral Leishmaniosis
Baço
Biópsia por Agulha
Leucócitos
Leishmaniose Visceral
dc.subject.en.pt_BR.fl_str_mv Spleen
Canine
Fine-needle Aspirative Biopsy
Leukocyte Populations
Canine Visceral Leishmaniosis
dc.subject.decs.pt_BR.fl_str_mv Baço
Biópsia por Agulha
Leucócitos
Leishmaniose Visceral
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2011-07-01T19:16:02Z
dc.date.available.fl_str_mv 2011-07-01T19:16:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv BARROUIN-MELO, S. M. et al. A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis. Veterinary Immunology and Immunopathology, v. 111, n. 3-4, p. 251-261, 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/2742
dc.identifier.issn.none.fl_str_mv 0165-2427
identifier_str_mv BARROUIN-MELO, S. M. et al. A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: assessment of leukocyte population changes in canine visceral leishmaniosis. Veterinary Immunology and Immunopathology, v. 111, n. 3-4, p. 251-261, 2006.
0165-2427
url https://www.arca.fiocruz.br/handle/icict/2742
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instacron_str FIOCRUZ
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