A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/6499 |
Resumo: | RESTRITO |
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Melo, Stella Maria BarrouinLarangeira, Daniela FariasSantos, Silvana OrnelasChagas Júnior, Adenizar Delgado dasPaixão, MarizaAguiar, Paulo Henrique PalisSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes deMelo, Stella Maria BarrouinLarangeira, Daniela FariasSantos, Silvana OrnelasChagas Júnior, Adenizar Delgado dasPaixão, MarizaAguiar, Paulo Henrique PalisSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes de2012-07-26T19:48:41Z2012-07-26T19:48:41Z20060165-2427http://www.repositorio.ufba.br/ri/handle/ri/6499v.111, n.3-4RESTRITOA method for the evaluation of splenic cellularity using samples collected by fine-needle aspirative biopsy was standardized in this work. The procedure includes erythrocyte lysing, preparation of cytospin films and staining by histochemical and immunocytochemical techniques. The cellular profiles of spleen preparations were compared with those observed in peripheral blood samples subjected to the same procedure. Two groups were compared, one consisting of 14 healthy uninfected and the other of 15 polysymptomatic Leishmania chagasi/infantum-infected dogs, from an endemic area for visceral leishmaniosis. Cell populations were identified by conventional hematoxilin–eosin and Wright’ stainings, and by immunocytochemistry using monoclonal antibodies against canine CD45RA and CD45RB, phagocytes and a pan-leukocyte antigen. Larger neutrophil (P < 0.0001) and monocyte/macrophage (P = 0.0036) relative counts and lower lymphocyte relative counts (P < 0.0001) were found in the spleen, and not in the blood, of the animals with leishmaniosis than in those of the healthy animals. The proportions of CD45RB+ cells were higher, and of CD45RA+ cells were lower, both in the spleen and in the blood of animals with leishmaniosis than in those of healthy dogs (P < 0.05). Additionally, hematoxilin–eosin-stained cytospins of spleen aspirates from Leishmania-infected animals permitted the easy visualization of amastigote forms inside phagocytes, under light microscopy.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2012-07-26T19:48:41Z No. of bitstreams: 1 GG.pdf: 337692 bytes, checksum: 085d3627daac4f4f547fcda56dfbede8 (MD5)Made available in DSpace on 2012-07-26T19:48:41Z (GMT). No. of bitstreams: 1 GG.pdf: 337692 bytes, checksum: 085d3627daac4f4f547fcda56dfbede8 (MD5) Previous issue date: 2006SalvadorVeterinary Immunology and Immunopathologyhttp://dx.doi.org/10.1016/j.vetimm.2006.01.021reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBASpleenCanineFine-needle aspirative biopsyLeukocyte populationsCanine visceral leishmaniosisA standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosisVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6499/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINALGG.pdfGG.pdfapplication/pdf337692https://repositorio.ufba.br/bitstream/ri/6499/1/GG.pdf085d3627daac4f4f547fcda56dfbede8MD51TEXTGG.pdf.txtGG.pdf.txtExtracted texttext/plain43460https://repositorio.ufba.br/bitstream/ri/6499/3/GG.pdf.txtec6659ed9b18ce1cc1e3135cccd49839MD53ri/64992022-07-05 14:03:10.535oai:repositorio.ufba.br:ri/6499VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:10Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| dc.title.alternative.pt_BR.fl_str_mv |
Veterinary Immunology and Immunopathology |
| title |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| spellingShingle |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis Melo, Stella Maria Barrouin Spleen Canine Fine-needle aspirative biopsy Leukocyte populations Canine visceral leishmaniosis |
| title_short |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| title_full |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| title_fullStr |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| title_full_unstemmed |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| title_sort |
A standardized cytological and immunochemical method for the analysis of fine-needle spleen aspirates: Assessment of leukocyte population changes in canine visceral leishmaniosis |
| author |
Melo, Stella Maria Barrouin |
| author_facet |
Melo, Stella Maria Barrouin Larangeira, Daniela Farias Santos, Silvana Ornelas Chagas Júnior, Adenizar Delgado das Paixão, Mariza Aguiar, Paulo Henrique Palis Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author_role |
author |
| author2 |
Larangeira, Daniela Farias Santos, Silvana Ornelas Chagas Júnior, Adenizar Delgado das Paixão, Mariza Aguiar, Paulo Henrique Palis Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Melo, Stella Maria Barrouin Larangeira, Daniela Farias Santos, Silvana Ornelas Chagas Júnior, Adenizar Delgado das Paixão, Mariza Aguiar, Paulo Henrique Palis Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de Melo, Stella Maria Barrouin Larangeira, Daniela Farias Santos, Silvana Ornelas Chagas Júnior, Adenizar Delgado das Paixão, Mariza Aguiar, Paulo Henrique Palis Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| dc.subject.por.fl_str_mv |
Spleen Canine Fine-needle aspirative biopsy Leukocyte populations Canine visceral leishmaniosis |
| topic |
Spleen Canine Fine-needle aspirative biopsy Leukocyte populations Canine visceral leishmaniosis |
| description |
RESTRITO |
| publishDate |
2006 |
| dc.date.issued.fl_str_mv |
2006 |
| dc.date.accessioned.fl_str_mv |
2012-07-26T19:48:41Z |
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2012-07-26T19:48:41Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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http://www.repositorio.ufba.br/ri/handle/ri/6499 |
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0165-2427 |
| dc.identifier.number.pt_BR.fl_str_mv |
v.111, n.3-4 |
| identifier_str_mv |
0165-2427 v.111, n.3-4 |
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http://www.repositorio.ufba.br/ri/handle/ri/6499 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
| dc.publisher.none.fl_str_mv |
Veterinary Immunology and Immunopathology |
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Veterinary Immunology and Immunopathology |
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http://dx.doi.org/10.1016/j.vetimm.2006.01.021 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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