Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41152 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. |
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Cunha, Clarissa F.Nogueira, Raquel FerrazCosta, Vanessa F. A.Pimentel, Maria Inês F.Chometon, Thaize Q.Lyra, Marcelo R.Schubach, Armando O.Cruz, Alda Maria daBertho, Álvaro Luiz2020-05-07T17:13:49Z2020-05-07T17:13:49Z2020CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, e0229400, 15p, Mar. 2020.1932-6203https://www.arca.fiocruz.br/handle/icict/4115210.1371/journal.pone.022940engPublic Library of ScienceLeishmania braziliensisImunopatogênese cutâneaAntígenosPacientesCitotóxicoPerfis de exaustãoLeishmania braziliensisImmunopathogenesisCuttaneous leishmaniasisCytotoxicExhaustion profilesantigenContribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profilesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa Médica Interdisciplinar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.The pathogenesis of cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) braziliensis is dictated mainly by the immune-mediated-tissue inflammation developed. The understanding of the immunological mechanisms that generate tissue damage or resolution of lesions is the key to the development of effective vaccine protocols and proper therapeutic schemes. It is clear that the specific immune response mediated by T cells is responsible for the beneficial outcome of the disease, however, the roles of CD4+ T, CD8+ T, NK and NKT cell subpopulations in immunopathogenesis of CL need to be elucidated. Peripheral blood cells from patients before, during and after the antimonial therapy, as well as healthy individuals (HI) were cultured with (LbAgS) or without (NS) L. braziliensis antigens (LbAg). Afterwards, the frequencies of LbAg-specific-cytotoxic CD8+ T, CD4+ T, NK and CD3+ CD56+ NKT cells, as well as their activation and exhaustion profiles, were defined by flow cytometry. We observed higher frequencies of CD8+ T, NK and CD3+ CD56+ NKT cells and lower frequencies of CD4+ T lymphocytes in LbAgS cell cultures from patients before treatment. The specific response to LbAg resulted in an expansion of cytotoxic-activated CD4+ T, CD8+ T, and NK cells, before and during treatment, indicating specificity in the response by these cells against L. braziliensis. Furthermore, comparing the differences of frequencies of cytotoxic-activated CD4+ T, CD8+ T, and NK cells, among before and during treatment patients and HI groups, we conclude that these cell populations are in charge of immune response elicited by antimonial therapy. Interestingly, we also observed that NK cells were induced by LbAg to an exhaustion profile during all clinical stages of the disease. The increased antigen-specific activation and cytotoxic activity are in line with the strong inflammatory response described in this disease, a likely cause of tissue damage. These findings reinforce the involvement of these distinct cytotoxic-activated cell populations in the immunopathogenesis of CL, showing a character of specificity in this immune response.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41152/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdfRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdfapplication/pdf2246103https://www.arca.fiocruz.br/bitstream/icict/41152/2/RaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdfd68e8d2930b15ff4a07eb9a7bac0c89aMD52TEXTRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdf.txtRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
title |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
spellingShingle |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles Cunha, Clarissa F. Leishmania braziliensis Imunopatogênese cutânea Antígenos Pacientes Citotóxico Perfis de exaustão Leishmania braziliensis Immunopathogenesis Cuttaneous leishmaniasis Cytotoxic Exhaustion profiles antigen |
title_short |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
title_full |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
title_fullStr |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
title_full_unstemmed |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
title_sort |
Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles |
author |
Cunha, Clarissa F. |
author_facet |
Cunha, Clarissa F. Nogueira, Raquel Ferraz Costa, Vanessa F. A. Pimentel, Maria Inês F. Chometon, Thaize Q. Lyra, Marcelo R. Schubach, Armando O. Cruz, Alda Maria da Bertho, Álvaro Luiz |
author_role |
author |
author2 |
Nogueira, Raquel Ferraz Costa, Vanessa F. A. Pimentel, Maria Inês F. Chometon, Thaize Q. Lyra, Marcelo R. Schubach, Armando O. Cruz, Alda Maria da Bertho, Álvaro Luiz |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Clarissa F. Nogueira, Raquel Ferraz Costa, Vanessa F. A. Pimentel, Maria Inês F. Chometon, Thaize Q. Lyra, Marcelo R. Schubach, Armando O. Cruz, Alda Maria da Bertho, Álvaro Luiz |
dc.subject.other.pt_BR.fl_str_mv |
Leishmania braziliensis Imunopatogênese cutânea Antígenos Pacientes Citotóxico Perfis de exaustão |
topic |
Leishmania braziliensis Imunopatogênese cutânea Antígenos Pacientes Citotóxico Perfis de exaustão Leishmania braziliensis Immunopathogenesis Cuttaneous leishmaniasis Cytotoxic Exhaustion profiles antigen |
dc.subject.en.pt_BR.fl_str_mv |
Leishmania braziliensis Immunopathogenesis Cuttaneous leishmaniasis Cytotoxic Exhaustion profiles antigen |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-05-07T17:13:49Z |
dc.date.available.fl_str_mv |
2020-05-07T17:13:49Z |
dc.date.issued.fl_str_mv |
2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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dc.identifier.citation.fl_str_mv |
CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, e0229400, 15p, Mar. 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/41152 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.022940 |
identifier_str_mv |
CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, e0229400, 15p, Mar. 2020. 1932-6203 10.1371/journal.pone.022940 |
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https://www.arca.fiocruz.br/handle/icict/41152 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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