Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles

Detalhes bibliográficos
Autor(a) principal: Cunha, Clarissa F.
Data de Publicação: 2020
Outros Autores: Nogueira, Raquel Ferraz, Costa, Vanessa F. A., Pimentel, Maria Inês F., Chometon, Thaize Q., Lyra, Marcelo R., Schubach, Armando O., Cruz, Alda Maria da, Bertho, Álvaro Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/41152
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Cunha, Clarissa F.Nogueira, Raquel FerrazCosta, Vanessa F. A.Pimentel, Maria Inês F.Chometon, Thaize Q.Lyra, Marcelo R.Schubach, Armando O.Cruz, Alda Maria daBertho, Álvaro Luiz2020-05-07T17:13:49Z2020-05-07T17:13:49Z2020CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, e0229400, 15p, Mar. 2020.1932-6203https://www.arca.fiocruz.br/handle/icict/4115210.1371/journal.pone.022940engPublic Library of ScienceLeishmania braziliensisImunopatogênese cutâneaAntígenosPacientesCitotóxicoPerfis de exaustãoLeishmania braziliensisImmunopathogenesisCuttaneous leishmaniasisCytotoxicExhaustion profilesantigenContribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profilesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Vigilância para Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa Médica Interdisciplinar. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Plataforma de Citometria de Fluxo. Rio de Janeiro, RJ, Brasil.The pathogenesis of cutaneous leishmaniasis (CL) caused by Leishmania (Viannia) braziliensis is dictated mainly by the immune-mediated-tissue inflammation developed. The understanding of the immunological mechanisms that generate tissue damage or resolution of lesions is the key to the development of effective vaccine protocols and proper therapeutic schemes. It is clear that the specific immune response mediated by T cells is responsible for the beneficial outcome of the disease, however, the roles of CD4+ T, CD8+ T, NK and NKT cell subpopulations in immunopathogenesis of CL need to be elucidated. Peripheral blood cells from patients before, during and after the antimonial therapy, as well as healthy individuals (HI) were cultured with (LbAgS) or without (NS) L. braziliensis antigens (LbAg). Afterwards, the frequencies of LbAg-specific-cytotoxic CD8+ T, CD4+ T, NK and CD3+ CD56+ NKT cells, as well as their activation and exhaustion profiles, were defined by flow cytometry. We observed higher frequencies of CD8+ T, NK and CD3+ CD56+ NKT cells and lower frequencies of CD4+ T lymphocytes in LbAgS cell cultures from patients before treatment. The specific response to LbAg resulted in an expansion of cytotoxic-activated CD4+ T, CD8+ T, and NK cells, before and during treatment, indicating specificity in the response by these cells against L. braziliensis. Furthermore, comparing the differences of frequencies of cytotoxic-activated CD4+ T, CD8+ T, and NK cells, among before and during treatment patients and HI groups, we conclude that these cell populations are in charge of immune response elicited by antimonial therapy. Interestingly, we also observed that NK cells were induced by LbAg to an exhaustion profile during all clinical stages of the disease. The increased antigen-specific activation and cytotoxic activity are in line with the strong inflammatory response described in this disease, a likely cause of tissue damage. These findings reinforce the involvement of these distinct cytotoxic-activated cell populations in the immunopathogenesis of CL, showing a character of specificity in this immune response.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41152/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdfRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdfapplication/pdf2246103https://www.arca.fiocruz.br/bitstream/icict/41152/2/RaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdfd68e8d2930b15ff4a07eb9a7bac0c89aMD52TEXTRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdf.txtRaquelNogueira_AldaMariaCruz_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
title Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
spellingShingle Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
Cunha, Clarissa F.
Leishmania braziliensis
Imunopatogênese cutânea
Antígenos
Pacientes
Citotóxico
Perfis de exaustão
Leishmania braziliensis
Immunopathogenesis
Cuttaneous leishmaniasis
Cytotoxic
Exhaustion profiles
antigen
title_short Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
title_full Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
title_fullStr Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
title_full_unstemmed Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
title_sort Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles
author Cunha, Clarissa F.
author_facet Cunha, Clarissa F.
Nogueira, Raquel Ferraz
Costa, Vanessa F. A.
Pimentel, Maria Inês F.
Chometon, Thaize Q.
Lyra, Marcelo R.
Schubach, Armando O.
Cruz, Alda Maria da
Bertho, Álvaro Luiz
author_role author
author2 Nogueira, Raquel Ferraz
Costa, Vanessa F. A.
Pimentel, Maria Inês F.
Chometon, Thaize Q.
Lyra, Marcelo R.
Schubach, Armando O.
Cruz, Alda Maria da
Bertho, Álvaro Luiz
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Clarissa F.
Nogueira, Raquel Ferraz
Costa, Vanessa F. A.
Pimentel, Maria Inês F.
Chometon, Thaize Q.
Lyra, Marcelo R.
Schubach, Armando O.
Cruz, Alda Maria da
Bertho, Álvaro Luiz
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Imunopatogênese cutânea
Antígenos
Pacientes
Citotóxico
Perfis de exaustão
topic Leishmania braziliensis
Imunopatogênese cutânea
Antígenos
Pacientes
Citotóxico
Perfis de exaustão
Leishmania braziliensis
Immunopathogenesis
Cuttaneous leishmaniasis
Cytotoxic
Exhaustion profiles
antigen
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Immunopathogenesis
Cuttaneous leishmaniasis
Cytotoxic
Exhaustion profiles
antigen
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-05-07T17:13:49Z
dc.date.available.fl_str_mv 2020-05-07T17:13:49Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, e0229400, 15p, Mar. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/41152
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.022940
identifier_str_mv CUNHA, Clarissa F. et al. Contribution of Leishmania braziliensis antigen-specific CD4+ T, CD8+ T, NK and CD3+ CD56+ NKT cells in the immunopathogenesis of cutaneous leishmaniasis patients: Cytotoxic, activation and exhaustion profiles. Plos One, v. 15, n. 3, e0229400, 15p, Mar. 2020.
1932-6203
10.1371/journal.pone.022940
url https://www.arca.fiocruz.br/handle/icict/41152
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