Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.

Detalhes bibliográficos
Autor(a) principal: Andrade, Sonia Gumes
Data de Publicação: 1991
Outros Autores: Guerret, Sylviane Stocker, Pimentel, Ariane R, Grimaud, Jean Alexis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/5711
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Andrade, Sonia GumesGuerret, Sylviane StockerPimentel, Ariane RGrimaud, Jean Alexis2012-10-22T20:37:09Z2012-10-22T20:37:09Z1991ANDRADE, S.G. et al. Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy. Memórias do Instituto Oswaldo Cruz, v. 86, n. 2, p. 187-200, apr.-jun. 1991.0074-0276https://www.arca.fiocruz.br/handle/icict/5711engReversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur de Lyon. Centro de Microscopie Eletronique. Lyon, FranceFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur de Lyon. Centro de Microscopie Eletronique. Lyon, FranceThis investigation was performed to verify the effect of specific chemotherapy (Benznidazole or MK-436) on the inflammatory and fibrotic cardiac alterations in mice chronically infected with the strains 21 SF (Type II) and Colombian (Type III) of Trypanosoma cruzi. To obtain chronically infected mice, two groups of 100 Swiss mice each, were infected with either the 21 SF or the Colombian strain (2 x 10(4) and 5 x 10(4) blood forms respectively). The rate of mortality in the acute phase was of 80% for both groups. Twenty surviving mice chronically infected with the 21 SF strain and 20 with the Colombian strain were then divided in treated and untreated groups. Excluding those that died during the course of treatment, 14 mice chronically infected with the 21 SF strain and 15 with the Colombian strain were finally evaluated in the present study. Chemotherapy was performed with Benznidazole (N-benzil-2-nitro-1-imidazolacetamide) in the dose of 100 mg/k.b.w/day, for 60 days, or with the MK-436 (3(1-methyl-5 nitroimidazol-2-yl) in two daily doses of 250 mg/k.b.w, for 20 days. Parasitological cure tests were performed (xenodiagnosis, haemoculture, subinoculation of the blood into newborn mice), and serological indirect immunofluorescence test. The treated and untreated mice as well as intact controls were killed at different periods after treatment and the heart were submitted to histopathological study with hematoxilineosin and picrosirius staining; ultrastructural study; collagen immunotyping, fibronectin and laminin identification by immunofluorescence tests. Results: the untreated controls either infected with 21 SF or Colombian strain, showed inflammatory and fibrotic alterations that were mild to moderate with the 21 SF strain and intense with the Colombian strain. Redpicrosirius staining showed bundles of collagen in the interstitial space and around cardiac fibers. Increased deposits of matritial components and collagen fibers, macrophages and fibroblasts appeared at the ultrastructural examination. Deposits of fibronectin, laminin, pro-III and IV collagens were seen, most intense in those infected with the Colombian strain. Treated mice, parasitologically cured, presented clear-cut regression of the inflammatory lesions and of the interstitial matrix thickening. Mice infected with the Colombian strain and treated with MK-436, was parasitologically cured in 5/6 cases and showed mild inflammatory infiltration and fibrosis. The mice treated with Benznidazole (Colombian strain) did not cure and showed moderate fibrosis and inflammation. Treatment of the mice infected with the 21 SF with Benznidazole determined parasitological cure of all animals, that showed mild inflammation and fibrosis of the myocardiumTrypanosoma cruziChronic myocarditisCollagen immunotypingFibronectinLamininChemotherapyCardiomiopatia Chagásica/quimioterapiaNitroimidazóis/uso terapêuticoAnimaisCardiomiopatia Chagásica/parasitologiaCardiomiopatia Chagásica/patologiaModelos Animais de DoençasFibrose Endomiocárdica/quimioterapiaFibrose Endomiocárdica/parasitologiaFibrose Endomiocárdica/patologiaMatriz Extracelular/ultraestruturaCamundongosMiocárdio/ultraestruturaEspecificidade da Espécieinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALAndrade SG Reversibility of cardiac fibrosis....pdfAndrade SG Reversibility of cardiac fibrosis....pdfapplication/pdf919087https://www.arca.fiocruz.br/bitstream/icict/5711/1/Andrade%20SG%20Reversibility%20of%20cardiac%20fibrosis....pdf513bea7797956d190b9fc2c073126ad5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-82008https://www.arca.fiocruz.br/bitstream/icict/5711/2/license.txt06db3f0df647fd9ddff657403bbe0ee2MD52TEXTAndrade SG Reversibility of cardiac fibrosis....pdf.txtAndrade SG Reversibility of cardiac fibrosis....pdf.txtExtracted texttext/plain14https://www.arca.fiocruz.br/bitstream/icict/5711/5/Andrade%20SG%20Reversibility%20of%20cardiac%20fibrosis....pdf.txt06b7e51e8fc077b8c75076712e4dd2b3MD55THUMBNAILAndrade SG Reversibility of cardiac fibrosis....pdf.jpgAndrade SG Reversibility of cardiac fibrosis....pdf.jpgGenerated Thumbnailimage/jpeg2510https://www.arca.fiocruz.br/bitstream/icict/5711/4/Andrade%20SG%20Reversibility%20of%20cardiac%20fibrosis....pdf.jpgf7f0419f7c379e9e842850c303a082caMD54icict/57112023-03-15 14:33:03.831oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
title Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
spellingShingle Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
Andrade, Sonia Gumes
Trypanosoma cruzi
Chronic myocarditis
Collagen immunotyping
Fibronectin
Laminin
Chemotherapy
Cardiomiopatia Chagásica/quimioterapia
Nitroimidazóis/uso terapêutico
Animais
Cardiomiopatia Chagásica/parasitologia
Cardiomiopatia Chagásica/patologia
Modelos Animais de Doenças
Fibrose Endomiocárdica/quimioterapia
Fibrose Endomiocárdica/parasitologia
Fibrose Endomiocárdica/patologia
Matriz Extracelular/ultraestrutura
Camundongos
Miocárdio/ultraestrutura
Especificidade da Espécie
title_short Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
title_full Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
title_fullStr Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
title_full_unstemmed Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
title_sort Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy.
author Andrade, Sonia Gumes
author_facet Andrade, Sonia Gumes
Guerret, Sylviane Stocker
Pimentel, Ariane R
Grimaud, Jean Alexis
author_role author
author2 Guerret, Sylviane Stocker
Pimentel, Ariane R
Grimaud, Jean Alexis
author2_role author
author
author
dc.contributor.author.fl_str_mv Andrade, Sonia Gumes
Guerret, Sylviane Stocker
Pimentel, Ariane R
Grimaud, Jean Alexis
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Chronic myocarditis
Collagen immunotyping
Fibronectin
Laminin
Chemotherapy
topic Trypanosoma cruzi
Chronic myocarditis
Collagen immunotyping
Fibronectin
Laminin
Chemotherapy
Cardiomiopatia Chagásica/quimioterapia
Nitroimidazóis/uso terapêutico
Animais
Cardiomiopatia Chagásica/parasitologia
Cardiomiopatia Chagásica/patologia
Modelos Animais de Doenças
Fibrose Endomiocárdica/quimioterapia
Fibrose Endomiocárdica/parasitologia
Fibrose Endomiocárdica/patologia
Matriz Extracelular/ultraestrutura
Camundongos
Miocárdio/ultraestrutura
Especificidade da Espécie
dc.subject.decs.pt_BR.fl_str_mv Cardiomiopatia Chagásica/quimioterapia
Nitroimidazóis/uso terapêutico
Animais
Cardiomiopatia Chagásica/parasitologia
Cardiomiopatia Chagásica/patologia
Modelos Animais de Doenças
Fibrose Endomiocárdica/quimioterapia
Fibrose Endomiocárdica/parasitologia
Fibrose Endomiocárdica/patologia
Matriz Extracelular/ultraestrutura
Camundongos
Miocárdio/ultraestrutura
Especificidade da Espécie
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 1991
dc.date.issued.fl_str_mv 1991
dc.date.accessioned.fl_str_mv 2012-10-22T20:37:09Z
dc.date.available.fl_str_mv 2012-10-22T20:37:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE, S.G. et al. Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy. Memórias do Instituto Oswaldo Cruz, v. 86, n. 2, p. 187-200, apr.-jun. 1991.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/5711
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv ANDRADE, S.G. et al. Reversibility of cardiac fibrosis in mice chronically infected with Trypanosoma cruzi, under specific chemotherapy. Memórias do Instituto Oswaldo Cruz, v. 86, n. 2, p. 187-200, apr.-jun. 1991.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/5711
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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