SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23842 |
Resumo: | University of Toledo College of Medicine and Life Sciences. Department of Medical Microbiology and Immunology. Belo Horizonte, MG, Brasil. |
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Ferreira, Viviane P.Vale, Vladimir FazitoPangburn, Michael K.Abdeladhim, MahaSousa, Antonio Ferreira MendesAbreu, Iliano V. CoutinhoRasouli, ManoochehrBrandt, Elizabeth A.Meneses, ClaudioLima, Kolyvan FerreiraAraujo, Ricardo NascimentoPereira, Marcos HorácioKotsyfakis, MichalisOliveira, FabianoKamhawi, ShadenRibeiro, José M. C.Gontijo, Neider F.Collin, NicolasValenzuela, Jesus G.2018-01-02T13:02:13Z2018-01-02T13:02:13Z2016FERREIRA, Viviane P. et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Scientific Reports, v.6:19300, 13p, Jan. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/2384210.1038/srep19300engNature Publishing GroupLutzomyia longipalpisTerapiaInibidorSalivaLutzomyia longipalpisinhibitorsalivaSALOTherapySALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Toledo College of Medicine and Life Sciences. Department of Medical Microbiology and Immunology. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais, Departamento de Parasitologia. Belo Horizonte, MG, Brasil.The University of Texas Health Science Center at Tyler. Tyler, TX, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais, Departamento de Parasitologia. Belo Horizonte, MG, Brasil / National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrasilBiology Center of the Czech Academy of Sciences. Budweis,CZ-370 05, Czech Republic.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais, Departamento de Parasitologia. Belo Horizonte, MG, Brasil.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA / University of Lausanne. Department of Biochemistry. Vaccine Formulation Laboratory. Switzerland.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Blood-feeding insects inject potent salivary components including complement inhibitors into their host’s skin to acquire a blood meal. Sand fly saliva was shown to inhibit the classical pathway of complement; however, the molecular identity of the inhibitor remains unknown. Here, we identified SALO as the classical pathway complement inhibitor. SALO, an 11kDa protein, has no homology to proteins of any other organism apart from New World sand flies. rSALO anti-complement activity has the same chromatographic properties as the Lu. longipalpis salivary gland homogenate (SGH) counterparts and anti-rSALO antibodies blocked the classical pathway complement activity of rSALO and SGH. Both rSALO and SGH inhibited C4b deposition and cleavage of C4. rSALO, however, did not inhibit the protease activity of C1s nor the enzymatic activity of factor Xa, uPA, thrombin, kallikrein, trypsin and plasmin. Importantly, rSALO did not inhibit the alternative or the lectin pathway of complement. In conclusion our data shows that SALO is a specific classical pathway complement inhibitor present in the saliva of Lu. longipalpis. Importantly, due to its small size and specificity, SALO may offer a therapeutic alternative for complement classical pathway-mediated pathogenic effects in human diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23842/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALvladimirfazitovale_etal_IOC_2016.pdfvladimirfazitovale_etal_IOC_2016.pdfapplication/pdf1475627https://www.arca.fiocruz.br/bitstream/icict/23842/2/vladimirfazitovale_etal_IOC_2016.pdfc1953ee0c69bc7cd67d9facdf9a9889aMD52TEXTvladimirfazitovale_etal_IOC_2016.pdf.txtvladimirfazitovale_etal_IOC_2016.pdf.txtExtracted texttext/plain56710https://www.arca.fiocruz.br/bitstream/icict/23842/3/vladimirfazitovale_etal_IOC_2016.pdf.txtfc5b8785e8513b4f58f49667e51369efMD53icict/238422018-08-15 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| dc.title.pt_BR.fl_str_mv |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| title |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| spellingShingle |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis Ferreira, Viviane P. Lutzomyia longipalpis Terapia Inibidor Saliva Lutzomyia longipalpis inhibitor saliva SALO Therapy |
| title_short |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| title_full |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| title_fullStr |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| title_full_unstemmed |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| title_sort |
SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis |
| author |
Ferreira, Viviane P. |
| author_facet |
Ferreira, Viviane P. Vale, Vladimir Fazito Pangburn, Michael K. Abdeladhim, Maha Sousa, Antonio Ferreira Mendes Abreu, Iliano V. Coutinho Rasouli, Manoochehr Brandt, Elizabeth A. Meneses, Claudio Lima, Kolyvan Ferreira Araujo, Ricardo Nascimento Pereira, Marcos Horácio Kotsyfakis, Michalis Oliveira, Fabiano Kamhawi, Shaden Ribeiro, José M. C. Gontijo, Neider F. Collin, Nicolas Valenzuela, Jesus G. |
| author_role |
author |
| author2 |
Vale, Vladimir Fazito Pangburn, Michael K. Abdeladhim, Maha Sousa, Antonio Ferreira Mendes Abreu, Iliano V. Coutinho Rasouli, Manoochehr Brandt, Elizabeth A. Meneses, Claudio Lima, Kolyvan Ferreira Araujo, Ricardo Nascimento Pereira, Marcos Horácio Kotsyfakis, Michalis Oliveira, Fabiano Kamhawi, Shaden Ribeiro, José M. C. Gontijo, Neider F. Collin, Nicolas Valenzuela, Jesus G. |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Viviane P. Vale, Vladimir Fazito Pangburn, Michael K. Abdeladhim, Maha Sousa, Antonio Ferreira Mendes Abreu, Iliano V. Coutinho Rasouli, Manoochehr Brandt, Elizabeth A. Meneses, Claudio Lima, Kolyvan Ferreira Araujo, Ricardo Nascimento Pereira, Marcos Horácio Kotsyfakis, Michalis Oliveira, Fabiano Kamhawi, Shaden Ribeiro, José M. C. Gontijo, Neider F. Collin, Nicolas Valenzuela, Jesus G. |
| dc.subject.other.pt_BR.fl_str_mv |
Lutzomyia longipalpis Terapia Inibidor Saliva |
| topic |
Lutzomyia longipalpis Terapia Inibidor Saliva Lutzomyia longipalpis inhibitor saliva SALO Therapy |
| dc.subject.en.pt_BR.fl_str_mv |
Lutzomyia longipalpis inhibitor saliva SALO Therapy |
| description |
University of Toledo College of Medicine and Life Sciences. Department of Medical Microbiology and Immunology. Belo Horizonte, MG, Brasil. |
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2016 |
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2016 |
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2018-01-02T13:02:13Z |
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2018-01-02T13:02:13Z |
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FERREIRA, Viviane P. et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Scientific Reports, v.6:19300, 13p, Jan. 2016. |
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https://www.arca.fiocruz.br/handle/icict/23842 |
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2045-2322 |
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10.1038/srep19300 |
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FERREIRA, Viviane P. et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Scientific Reports, v.6:19300, 13p, Jan. 2016. 2045-2322 10.1038/srep19300 |
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