SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis

Detalhes bibliográficos
Autor(a) principal: Ferreira, Viviane P.
Data de Publicação: 2016
Outros Autores: Vale, Vladimir Fazito, Pangburn, Michael K., Abdeladhim, Maha, Sousa, Antonio Ferreira Mendes, Abreu, Iliano V. Coutinho, Rasouli, Manoochehr, Brandt, Elizabeth A., Meneses, Claudio, Lima, Kolyvan Ferreira, Araujo, Ricardo Nascimento, Pereira, Marcos Horácio, Kotsyfakis, Michalis, Oliveira, Fabiano, Kamhawi, Shaden, Ribeiro, José M. C., Gontijo, Neider F., Collin, Nicolas, Valenzuela, Jesus G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23842
Resumo: University of Toledo College of Medicine and Life Sciences. Department of Medical Microbiology and Immunology. Belo Horizonte, MG, Brasil.
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spelling Ferreira, Viviane P.Vale, Vladimir FazitoPangburn, Michael K.Abdeladhim, MahaSousa, Antonio Ferreira MendesAbreu, Iliano V. CoutinhoRasouli, ManoochehrBrandt, Elizabeth A.Meneses, ClaudioLima, Kolyvan FerreiraAraujo, Ricardo NascimentoPereira, Marcos HorácioKotsyfakis, MichalisOliveira, FabianoKamhawi, ShadenRibeiro, José M. C.Gontijo, Neider F.Collin, NicolasValenzuela, Jesus G.2018-01-02T13:02:13Z2018-01-02T13:02:13Z2016FERREIRA, Viviane P. et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Scientific Reports, v.6:19300, 13p, Jan. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/2384210.1038/srep19300engNature Publishing GroupLutzomyia longipalpisTerapiaInibidorSalivaLutzomyia longipalpisinhibitorsalivaSALOTherapySALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Toledo College of Medicine and Life Sciences. Department of Medical Microbiology and Immunology. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais, Departamento de Parasitologia. Belo Horizonte, MG, Brasil.The University of Texas Health Science Center at Tyler. Tyler, TX, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais, Departamento de Parasitologia. Belo Horizonte, MG, Brasil / National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Laboratório de Fisiologia de Insetos Hematófagos. Belo Horizonte, MG, BrasilBiology Center of the Czech Academy of Sciences. Budweis,CZ-370 05, Czech Republic.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Universidade Federal de Minas Gerais, Departamento de Parasitologia. Belo Horizonte, MG, Brasil.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA / University of Lausanne. Department of Biochemistry. Vaccine Formulation Laboratory. Switzerland.National Institute of Health. National Institute of Allergy and Infectious Diseases. LMVR. Vector Molecular Biology Section. Rockville, MD, USA.Blood-feeding insects inject potent salivary components including complement inhibitors into their host’s skin to acquire a blood meal. Sand fly saliva was shown to inhibit the classical pathway of complement; however, the molecular identity of the inhibitor remains unknown. Here, we identified SALO as the classical pathway complement inhibitor. SALO, an 11kDa protein, has no homology to proteins of any other organism apart from New World sand flies. rSALO anti-complement activity has the same chromatographic properties as the Lu. longipalpis salivary gland homogenate (SGH) counterparts and anti-rSALO antibodies blocked the classical pathway complement activity of rSALO and SGH. Both rSALO and SGH inhibited C4b deposition and cleavage of C4. rSALO, however, did not inhibit the protease activity of C1s nor the enzymatic activity of factor Xa, uPA, thrombin, kallikrein, trypsin and plasmin. Importantly, rSALO did not inhibit the alternative or the lectin pathway of complement. In conclusion our data shows that SALO is a specific classical pathway complement inhibitor present in the saliva of Lu. longipalpis. Importantly, due to its small size and specificity, SALO may offer a therapeutic alternative for complement classical pathway-mediated pathogenic effects in human diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23842/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALvladimirfazitovale_etal_IOC_2016.pdfvladimirfazitovale_etal_IOC_2016.pdfapplication/pdf1475627https://www.arca.fiocruz.br/bitstream/icict/23842/2/vladimirfazitovale_etal_IOC_2016.pdfc1953ee0c69bc7cd67d9facdf9a9889aMD52TEXTvladimirfazitovale_etal_IOC_2016.pdf.txtvladimirfazitovale_etal_IOC_2016.pdf.txtExtracted texttext/plain56710https://www.arca.fiocruz.br/bitstream/icict/23842/3/vladimirfazitovale_etal_IOC_2016.pdf.txtfc5b8785e8513b4f58f49667e51369efMD53icict/238422018-08-15 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dc.title.pt_BR.fl_str_mv SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
title SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
spellingShingle SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
Ferreira, Viviane P.
Lutzomyia longipalpis
Terapia
Inibidor
Saliva
Lutzomyia longipalpis
inhibitor
saliva
SALO
Therapy
title_short SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
title_full SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
title_fullStr SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
title_full_unstemmed SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
title_sort SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis
author Ferreira, Viviane P.
author_facet Ferreira, Viviane P.
Vale, Vladimir Fazito
Pangburn, Michael K.
Abdeladhim, Maha
Sousa, Antonio Ferreira Mendes
Abreu, Iliano V. Coutinho
Rasouli, Manoochehr
Brandt, Elizabeth A.
Meneses, Claudio
Lima, Kolyvan Ferreira
Araujo, Ricardo Nascimento
Pereira, Marcos Horácio
Kotsyfakis, Michalis
Oliveira, Fabiano
Kamhawi, Shaden
Ribeiro, José M. C.
Gontijo, Neider F.
Collin, Nicolas
Valenzuela, Jesus G.
author_role author
author2 Vale, Vladimir Fazito
Pangburn, Michael K.
Abdeladhim, Maha
Sousa, Antonio Ferreira Mendes
Abreu, Iliano V. Coutinho
Rasouli, Manoochehr
Brandt, Elizabeth A.
Meneses, Claudio
Lima, Kolyvan Ferreira
Araujo, Ricardo Nascimento
Pereira, Marcos Horácio
Kotsyfakis, Michalis
Oliveira, Fabiano
Kamhawi, Shaden
Ribeiro, José M. C.
Gontijo, Neider F.
Collin, Nicolas
Valenzuela, Jesus G.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Viviane P.
Vale, Vladimir Fazito
Pangburn, Michael K.
Abdeladhim, Maha
Sousa, Antonio Ferreira Mendes
Abreu, Iliano V. Coutinho
Rasouli, Manoochehr
Brandt, Elizabeth A.
Meneses, Claudio
Lima, Kolyvan Ferreira
Araujo, Ricardo Nascimento
Pereira, Marcos Horácio
Kotsyfakis, Michalis
Oliveira, Fabiano
Kamhawi, Shaden
Ribeiro, José M. C.
Gontijo, Neider F.
Collin, Nicolas
Valenzuela, Jesus G.
dc.subject.other.pt_BR.fl_str_mv Lutzomyia longipalpis
Terapia
Inibidor
Saliva
topic Lutzomyia longipalpis
Terapia
Inibidor
Saliva
Lutzomyia longipalpis
inhibitor
saliva
SALO
Therapy
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpis
inhibitor
saliva
SALO
Therapy
description University of Toledo College of Medicine and Life Sciences. Department of Medical Microbiology and Immunology. Belo Horizonte, MG, Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2018-01-02T13:02:13Z
dc.date.available.fl_str_mv 2018-01-02T13:02:13Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Viviane P. et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Scientific Reports, v.6:19300, 13p, Jan. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23842
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/srep19300
identifier_str_mv FERREIRA, Viviane P. et al. SALO, a novel classical pathway complement inhibitor from saliva of the sand fly Lutzomyia longipalpis. Scientific Reports, v.6:19300, 13p, Jan. 2016.
2045-2322
10.1038/srep19300
url https://www.arca.fiocruz.br/handle/icict/23842
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Nature Publishing Group
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