An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9409 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. |
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Jangola, Soraya Torres GazeDriguez, PatrickPearson, Mark S.Mendes, TiagoDoolan, Denise L.Trieu, AngelaMcManus, Donald P.Gobert, Geoffrey N.Periago, Maria VictoriaOliveira, Rodrigo Corrêa deCardoso, Fernanda C.Oliveira, Guilherme Corrêa deNakajima, RieJasinskas, AlHung, ChrisLiang, LiPablo, JozelynBethony, Jeffrey MichaelFelgner, Philip L.Loukas, Alex2015-02-02T15:57:22Z2015-02-02T15:57:22Z2014GAZE, Soraya Torres et al. An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas. PLoS Pathogens, v. 10, n. 3, p. 1-16, 2014.1553-7366https://www.arca.fiocruz.br/handle/icict/940910.1371/journal.ppat.1004033engPublic Library of ScienceAn Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.QIMR Berghofer Medical Research Institute. Brisbane, Queensland, Australia.James Cook University.Australian Institute of Tropical Health and Medicine. Centre for Biodiscovery and Molecular Development of Therapeutics. Queensland Tropical Health Alliance Laboratory Cairns, Queensland, Australia.Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.QIMR Berghofer Medical Research Institute. Brisbane, Queensland, Australia.QIMR Berghofer Medical Research Institute. Brisbane, Queensland, Australia.QIMR Berghofer Medical Research Institute. Brisbane, Queensland, Australia.QIMR Berghofer Medical Research Institute. Brisbane, Queensland, Australia.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.QIMR Berghofer Medical Research Institute. Brisbane, Queensland, Australia / The University of Queensland. Institute for Molecular Biosciences. St Lucia, Queensland, Australia.Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil.University of California Irvine. Irvine, CA, USA.University of California Irvine. Irvine, CA, USA.University of California Irvine. Irvine, CA, USA.University of California Irvine. Irvine, CA, USA.University of California Irvine. Irvine, CA, USA.George Washington University. Washington, D.C., USA.University of California Irvine. Irvine, CA, USA.James Cook University.Australian Institute of Tropical Health and Medicine. Centre for Biodiscovery and Molecular Development of Therapeutics. Queensland Tropical Health Alliance Laboratory Cairns, Queensland, Australia.Schistosomiasis is a neglected tropical disease that is responsible for almost 300,000 deaths annually. Mass drug administration (MDA) is used worldwide for the control of schistosomiasis, but chemotherapy fails to prevent reinfection with schistosomes, so MDA alone is not sufficient to eliminate the disease, and a prophylactic vaccine is required. Herein, we take advantage of recent advances in systems biology and longitudinal studies in schistosomiasis endemic areas in Brazil to pilot an immunomics approach to the discovery of schistosomiasis vaccine antigens. We selected mostly surface-derived proteins, produced them using an in vitro rapid translation system and then printed them to generate the first protein microarray for a multi-cellular pathogen. Using well-established Brazilian cohorts of putatively resistant (PR) and chronically infected (CI) individuals stratified by the intensity of their S. mansoni infection, we probed arrays for IgG subclass and IgE responses to these antigens to detect antibody signatures that were reflective of protective vs. non-protective immune responses. Moreover, probing for IgE responses allowed us to identify antigens that might induce potentially deleterious hypersensitivity responses if used as subunit vaccines in endemic populations. Using multi-dimensional cluster analysis we showed that PR individuals mounted a distinct and robust IgG1 response to a small set of newly discovered and well-characterized surface (tegument) antigens in contrast to CI individuals who mounted strong IgE and IgG4 responses to many antigens. Herein, we show the utility of a vaccinomics approach that profiles antibody responses of resistant individuals in a high-throughput multiplex approach for the identification of several potentially protective and safe schistosomiasis vaccine antigens.AntibodiesHelminthDisease ResistanceHigh-Throughput Screening AssaysSchistosomiasisImmunologyEpidemiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9409/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_021.pdf2014_021.pdfapplication/pdf1697174https://www.arca.fiocruz.br/bitstream/icict/9409/2/2014_021.pdff663b94fdeeb56eed842086bf8eaaf4eMD52TEXT2014_021.pdf.txt2014_021.pdf.txtExtracted texttext/plain75143https://www.arca.fiocruz.br/bitstream/icict/9409/3/2014_021.pdf.txta43049fd9fec387e3bd788fc38dde626MD53icict/94092019-06-21 22:17:53.606oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-22T01:17:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| title |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| spellingShingle |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas Jangola, Soraya Torres Gaze Antibodies Helminth Disease Resistance High-Throughput Screening Assays Schistosomiasis Immunology Epidemiology |
| title_short |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| title_full |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| title_fullStr |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| title_full_unstemmed |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| title_sort |
An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas |
| author |
Jangola, Soraya Torres Gaze |
| author_facet |
Jangola, Soraya Torres Gaze Driguez, Patrick Pearson, Mark S. Mendes, Tiago Doolan, Denise L. Trieu, Angela McManus, Donald P. Gobert, Geoffrey N. Periago, Maria Victoria Oliveira, Rodrigo Corrêa de Cardoso, Fernanda C. Oliveira, Guilherme Corrêa de Nakajima, Rie Jasinskas, Al Hung, Chris Liang, Li Pablo, Jozelyn Bethony, Jeffrey Michael Felgner, Philip L. Loukas, Alex |
| author_role |
author |
| author2 |
Driguez, Patrick Pearson, Mark S. Mendes, Tiago Doolan, Denise L. Trieu, Angela McManus, Donald P. Gobert, Geoffrey N. Periago, Maria Victoria Oliveira, Rodrigo Corrêa de Cardoso, Fernanda C. Oliveira, Guilherme Corrêa de Nakajima, Rie Jasinskas, Al Hung, Chris Liang, Li Pablo, Jozelyn Bethony, Jeffrey Michael Felgner, Philip L. Loukas, Alex |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Jangola, Soraya Torres Gaze Driguez, Patrick Pearson, Mark S. Mendes, Tiago Doolan, Denise L. Trieu, Angela McManus, Donald P. Gobert, Geoffrey N. Periago, Maria Victoria Oliveira, Rodrigo Corrêa de Cardoso, Fernanda C. Oliveira, Guilherme Corrêa de Nakajima, Rie Jasinskas, Al Hung, Chris Liang, Li Pablo, Jozelyn Bethony, Jeffrey Michael Felgner, Philip L. Loukas, Alex |
| dc.subject.en.pt_BR.fl_str_mv |
Antibodies Helminth Disease Resistance High-Throughput Screening Assays Schistosomiasis Immunology Epidemiology |
| topic |
Antibodies Helminth Disease Resistance High-Throughput Screening Assays Schistosomiasis Immunology Epidemiology |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. |
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2014 |
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2014 |
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2015-02-02T15:57:22Z |
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2015-02-02T15:57:22Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
GAZE, Soraya Torres et al. An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas. PLoS Pathogens, v. 10, n. 3, p. 1-16, 2014. |
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https://www.arca.fiocruz.br/handle/icict/9409 |
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1553-7366 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.ppat.1004033 |
| identifier_str_mv |
GAZE, Soraya Torres et al. An Immunomics Approach to Schistosome Antigen Discovery: Antibody Signatures of Naturally Resistant and Chronically Infected Individuals from Endemic Areas. PLoS Pathogens, v. 10, n. 3, p. 1-16, 2014. 1553-7366 10.1371/journal.ppat.1004033 |
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Public Library of Science |
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Public Library of Science |
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