Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28947 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. |
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Gumarães, Michelle Lopes RibeiroMarengo, Eliana BliniTempone, Antonio JorgeAmaral, Julio JablonskiKlitzke, Clécio F.Silveira, Erika K. Xavier daPortaro, Fernanda Calheta VieiraPessolani, Maria Cristina V.2018-09-20T15:53:44Z2018-09-20T15:53:44Z2009GUIMARÃES, Michelle Lopes Ribeiro; et al. Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae. Mem Inst Oswaldo Cruz, v.104, n.8, p.1132-1138, Dec. 2009.0074-0276https://www.arca.fiocruz.br/handle/icict/289471678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzMycobacterium lepraeatividade enzimáticapatogêneseproteasePeptídeosMycobacterium lepraeHtr2proteaseenzymatic activityFRET peptidespathogenesisCloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium lepraeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Centro de Toxinologia Aplicada. Laboratório de Imunoquímica e LETA. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Centro de Toxinologia Aplicada. Laboratório de Imunoquímica e LETA. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Centro de Toxinologia Aplicada. Laboratório de Imunoquímica e LETA. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Members of the high temperature requirement A (HtrA) family of chaperone proteases have been shown to play a role in bacterial pathogenesis. In a recent report, we demonstrated that the gene ML0176, which codes for a predicted HtrA-like protease, a gene conserved in other species of mycobacteria, is transcribed by Mycobacterium leprae in human leprosy lesions. In the present study, the recombinant ML0176 protein was produced and its enzymatic properties investigated. M. leprae recombinant ML0176 was able to hydrolyse a variety of synthetic and natural peptides. Similar to other HtrA proteins, this enzyme displayed maximum proteolytic activity at temperatures above 40°C and was completely inactivated by aprotinin, a protease inhibitor with high selectivity for serine proteases. Finally, analysis of M. leprae ML0176 specificity suggested a broader cleavage preference than that of previously described HtrAs homologues. In summary, we have identified an HtrA-like protease in M. leprae that may constitute a potential new target for the development of novel prophylactic and/or therapeutic strategies against mycobacterial infections.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28947/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmichelle_guimaraes_etal_IOC_2009.pdfmichelle_guimaraes_etal_IOC_2009.pdfapplication/pdf392876https://www.arca.fiocruz.br/bitstream/icict/28947/2/michelle_guimaraes_etal_IOC_2009.pdf50a27d42a52427a72a6ce58d27999bbeMD52TEXTmichelle_guimaraes_etal_IOC_2009.pdf.txtmichelle_guimaraes_etal_IOC_2009.pdf.txtExtracted texttext/plain34667https://www.arca.fiocruz.br/bitstream/icict/28947/3/michelle_guimaraes_etal_IOC_2009.pdf.txt149ff558f13c0c226d16d1e7ef09db31MD53icict/289472018-09-21 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| dc.title.pt_BR.fl_str_mv |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| title |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| spellingShingle |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae Gumarães, Michelle Lopes Ribeiro Mycobacterium leprae atividade enzimática patogênese protease Peptídeos Mycobacterium leprae Htr2 protease enzymatic activity FRET peptides pathogenesis |
| title_short |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| title_full |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| title_fullStr |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| title_full_unstemmed |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| title_sort |
Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae |
| author |
Gumarães, Michelle Lopes Ribeiro |
| author_facet |
Gumarães, Michelle Lopes Ribeiro Marengo, Eliana Blini Tempone, Antonio Jorge Amaral, Julio Jablonski Klitzke, Clécio F. Silveira, Erika K. Xavier da Portaro, Fernanda Calheta Vieira Pessolani, Maria Cristina V. |
| author_role |
author |
| author2 |
Marengo, Eliana Blini Tempone, Antonio Jorge Amaral, Julio Jablonski Klitzke, Clécio F. Silveira, Erika K. Xavier da Portaro, Fernanda Calheta Vieira Pessolani, Maria Cristina V. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gumarães, Michelle Lopes Ribeiro Marengo, Eliana Blini Tempone, Antonio Jorge Amaral, Julio Jablonski Klitzke, Clécio F. Silveira, Erika K. Xavier da Portaro, Fernanda Calheta Vieira Pessolani, Maria Cristina V. |
| dc.subject.other.pt_BR.fl_str_mv |
Mycobacterium leprae atividade enzimática patogênese protease Peptídeos |
| topic |
Mycobacterium leprae atividade enzimática patogênese protease Peptídeos Mycobacterium leprae Htr2 protease enzymatic activity FRET peptides pathogenesis |
| dc.subject.en.pt_BR.fl_str_mv |
Mycobacterium leprae Htr2 protease enzymatic activity FRET peptides pathogenesis |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil. |
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2009 |
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2009 |
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2018-09-20T15:53:44Z |
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2018-09-20T15:53:44Z |
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article |
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GUIMARÃES, Michelle Lopes Ribeiro; et al. Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae. Mem Inst Oswaldo Cruz, v.104, n.8, p.1132-1138, Dec. 2009. |
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https://www.arca.fiocruz.br/handle/icict/28947 |
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0074-0276 |
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1678-8060 |
| identifier_str_mv |
GUIMARÃES, Michelle Lopes Ribeiro; et al. Cloning, expression and characterisation of an HtrA-like serine protease produced in vivo by Mycobacterium leprae. Mem Inst Oswaldo Cruz, v.104, n.8, p.1132-1138, Dec. 2009. 0074-0276 1678-8060 |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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