Development of a membrane adsorber based capture step for the purification of yellow fever virus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32038 |
Resumo: | Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Bioquímica. Rio de Janeiro, RJ, Brasil. |
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Pato, Tânia P.Souza, Marta Cristina O.Silva, Andréa N. M. RPereira, Renata C.Silva, Marlon V.Caride, ElenaGaspar, Luciane P.Freire, Marcos S.Castilho, Leda R.2019-03-12T14:17:44Z2019-03-12T14:17:44Z2014PATO, Tânia P. et al. Development of a membrane adsorber based capture step for the purification of yellow fever virus. Vaccine, Amsterdam, v. 32, n. 24, p. 2789-2793, 2014.1873-2518https://www.arca.fiocruz.br/handle/icict/3203810.1016/j.vaccine.2014.02.036engDevelopment of a membrane adsorber based capture step for the purification of yellow fever virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. COPPE. Laboratório de Engenharia de Cultura Celular. Rio de Janeiro , RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Bioquímica. Rio de Janeiro, RJ, Brasil.Yellow fever (YF) is an endemic disease in some tropical areas of South America and Africa that presents lethality rate between 20 and 50%. There is no specific treatment and to control this disease a highly effective live-attenuated egg based vaccine is widely used for travelers and residents of areas where YF is endemic. However, recent reports of rare, sometimes fatal, adverse events post-vaccination have raised concerns. In order to increase safety records, alternative strategies should be considered, such as developing a new inactivated vaccine using a cell culture based technology, capable of meeting the demands in cases of epidemic. With this goal, the production of YF virus in Vero cells grown on microcarriers and its subsequent purification by chromatographic techniques was studied. In this work we investigate the capture step of the purification process of the YF virus. At first, virus stability was studied over a wide pH range, showing best results for the alkaline region. Considering this result and the pI of the envelope protein previously determined in silico, a strong anion exchanger was considered most suitable. Due to the easy scalability, simplicity to handle, absence of diffusional limitations and suitability for virus handling of membrane adsorbers, a Q membrane was evaluated. The amount of antigen adsorbed onto the membrane was investigated within the pH range for virus stability, and the best pH for virus adsorption was considered to be 8.5. Finally, studies on gradient and step elution allowed to determine the most adequate salt concentration for washing (0.15 M) and virus elution (0.30 M). Under these operating conditions, it was shown that this capture step is quite efficient, showing high product recovery (93.2 ± 30.3%) and efficient DNA clearance (0.9 ± 0.3 ng/dose).Yellow fever virusYellow feverEndemic diseaseEpidemicinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32038/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALF92-1-s2.0-S0264410X14002096-main.pdfapplication/pdf932295https://www.arca.fiocruz.br/bitstream/icict/32038/2/F92-1-s2.0-S0264410X14002096-main.pdfd13d12d84dc262973e841319a05fe6bcMD52TEXTF92-1-s2.0-S0264410X14002096-main.pdf.txtF92-1-s2.0-S0264410X14002096-main.pdf.txtExtracted texttext/plain22790https://www.arca.fiocruz.br/bitstream/icict/32038/3/F92-1-s2.0-S0264410X14002096-main.pdf.txtd7866a499d8d2c558c97c7d088ba5092MD53icict/320382019-04-04 02:05:16.295oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-04-04T05:05:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| title |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| spellingShingle |
Development of a membrane adsorber based capture step for the purification of yellow fever virus Pato, Tânia P. Yellow fever virus Yellow fever Endemic disease Epidemic |
| title_short |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| title_full |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| title_fullStr |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| title_full_unstemmed |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| title_sort |
Development of a membrane adsorber based capture step for the purification of yellow fever virus |
| author |
Pato, Tânia P. |
| author_facet |
Pato, Tânia P. Souza, Marta Cristina O. Silva, Andréa N. M. R Pereira, Renata C. Silva, Marlon V. Caride, Elena Gaspar, Luciane P. Freire, Marcos S. Castilho, Leda R. |
| author_role |
author |
| author2 |
Souza, Marta Cristina O. Silva, Andréa N. M. R Pereira, Renata C. Silva, Marlon V. Caride, Elena Gaspar, Luciane P. Freire, Marcos S. Castilho, Leda R. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pato, Tânia P. Souza, Marta Cristina O. Silva, Andréa N. M. R Pereira, Renata C. Silva, Marlon V. Caride, Elena Gaspar, Luciane P. Freire, Marcos S. Castilho, Leda R. |
| dc.subject.en.pt_BR.fl_str_mv |
Yellow fever virus Yellow fever Endemic disease Epidemic |
| topic |
Yellow fever virus Yellow fever Endemic disease Epidemic |
| description |
Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Bioquímica. Rio de Janeiro, RJ, Brasil. |
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2014 |
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2014 |
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2019-03-12T14:17:44Z |
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2019-03-12T14:17:44Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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PATO, Tânia P. et al. Development of a membrane adsorber based capture step for the purification of yellow fever virus. Vaccine, Amsterdam, v. 32, n. 24, p. 2789-2793, 2014. |
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https://www.arca.fiocruz.br/handle/icict/32038 |
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1873-2518 |
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10.1016/j.vaccine.2014.02.036 |
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PATO, Tânia P. et al. Development of a membrane adsorber based capture step for the purification of yellow fever virus. Vaccine, Amsterdam, v. 32, n. 24, p. 2789-2793, 2014. 1873-2518 10.1016/j.vaccine.2014.02.036 |
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