Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease

Detalhes bibliográficos
Autor(a) principal: Rezende, Izabela Maurício de
Data de Publicação: 2022
Outros Autores: Oliveira, Gabriela Fernanda Garcia, Costa, Thaís Alkifeles, Khan, Aslam, Pereira, Leonardo Soares, Santos, Tayrine Araújo, Alves, Pedro Augusto, Silva, Carlos Eduardo Calzavara, Martins Filho, Olindo Assis, Carvalho, Andréa Teixeira de, LaBeaud, Angelle Desiree, Drumond, Betânia Paiva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/56807
Resumo: Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/ Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA
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spelling Rezende, Izabela Maurício deOliveira, Gabriela Fernanda GarciaCosta, Thaís AlkifelesKhan, AslamPereira, Leonardo SoaresSantos, Tayrine AraújoAlves, Pedro AugustoSilva, Carlos Eduardo CalzavaraMartins Filho, Olindo AssisCarvalho, Andréa Teixeira deLaBeaud, Angelle DesireeDrumond, Betânia Paiva2023-02-01T14:07:57Z2023-02-01T14:07:57Z2022REZENDE, Izabela Maurício de et al. Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease. J Clin Microbiol., v. 60, n. 8, e0025422, 2022. doi: 10.1128/jcm.00254-22.0095-1137https://www.arca.fiocruz.br/handle/icict/56807engAmerican Society for MicrobiologyYellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/ Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USALaboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, BrazilLaboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, BrazilDepartment of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA.Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil.Eduardo de Menezes Hospital. Belo Horizonte, MG, Brazil.Immunology of Viruses Diseases. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Cellular and Molecular Immunology. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Integrated Group of Biomarkers Research. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Integrated Group of Biomarkers Research. René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA.Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil.Prior studies have demonstrated prolonged presence of yellow fever virus (YFV) RNA in saliva and urine as an alternative to serum. To investigate the presence of YFV RNA in urine, we used RT-PCR for YFV screening in 60 urine samples collected from a large cohort of naturally infected yellow fever (YF) patients during acute and convalescent phases of YF infection from recent YF outbreaks in Brazil (2017 to 2018). Fifteen urine samples from acute phase infection (up to 15 days post-symptom onset) and four urine samples from convalescent phase infection (up to 69 days post-symptom onset), were YFV PCR-positive. We genotyped YFV detected in seven urine samples (five collected during the acute phase and two collected during the YF convalescent phase). Genotyping indicated the presence of YFV South American I genotype in these samples. To our knowledge, this is the first report of wild-type YFV RNA detection in the urine this far out from symptom onset (up to 69 DPS), including YFV RNA detection during the convalescent phase of YF infection. The detection of YFV RNA in urine is an indicative of YFV infection; however, the results of RT-PCR using urine as sample should be interpreted with care, since a negative result does not exclude the possibility of YFV infection. With a possible prolonged period of detection beyond the viremic phase, the use of urine samples coupled with serological tests, epidemiologic inquiry, and clinical assessment could provide a longer diagnostic window for laboratory YF diagnosis.diagnosisurinevirus surveillanceyellow feveryellow fever vírusinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56807/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALYellow Fever Molecular Diagnosis Using Urine Specimens during Acute.pdfYellow Fever Molecular Diagnosis Using Urine Specimens during Acute.pdfapplication/pdf1148160https://www.arca.fiocruz.br/bitstream/icict/56807/2/Yellow%20Fever%20Molecular%20Diagnosis%20Using%20Urine%20Specimens%20during%20Acute.pdf86c71e3b836a13c3068208b2df2971a8MD52icict/568072023-02-01 11:07:58.738oai:www.arca.fiocruz.br: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ório 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dc.title.en_US.fl_str_mv Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
title Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
spellingShingle Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
Rezende, Izabela Maurício de
diagnosis
urine
virus surveillance
yellow fever
yellow fever vírus
title_short Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
title_full Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
title_fullStr Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
title_full_unstemmed Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
title_sort Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease
author Rezende, Izabela Maurício de
author_facet Rezende, Izabela Maurício de
Oliveira, Gabriela Fernanda Garcia
Costa, Thaís Alkifeles
Khan, Aslam
Pereira, Leonardo Soares
Santos, Tayrine Araújo
Alves, Pedro Augusto
Silva, Carlos Eduardo Calzavara
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
LaBeaud, Angelle Desiree
Drumond, Betânia Paiva
author_role author
author2 Oliveira, Gabriela Fernanda Garcia
Costa, Thaís Alkifeles
Khan, Aslam
Pereira, Leonardo Soares
Santos, Tayrine Araújo
Alves, Pedro Augusto
Silva, Carlos Eduardo Calzavara
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
LaBeaud, Angelle Desiree
Drumond, Betânia Paiva
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rezende, Izabela Maurício de
Oliveira, Gabriela Fernanda Garcia
Costa, Thaís Alkifeles
Khan, Aslam
Pereira, Leonardo Soares
Santos, Tayrine Araújo
Alves, Pedro Augusto
Silva, Carlos Eduardo Calzavara
Martins Filho, Olindo Assis
Carvalho, Andréa Teixeira de
LaBeaud, Angelle Desiree
Drumond, Betânia Paiva
dc.subject.en.en_US.fl_str_mv diagnosis
urine
virus surveillance
yellow fever
yellow fever vírus
topic diagnosis
urine
virus surveillance
yellow fever
yellow fever vírus
description Laboratory of Viruses. Microbiology Department. Biological Sciences Institute. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/ Department of Pediatrics. Division of Infectious Disease. Stanford University School of Medicinegrid. Stanford, CA, USA
publishDate 2022
dc.date.issued.fl_str_mv 2022
dc.date.accessioned.fl_str_mv 2023-02-01T14:07:57Z
dc.date.available.fl_str_mv 2023-02-01T14:07:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv REZENDE, Izabela Maurício de et al. Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease. J Clin Microbiol., v. 60, n. 8, e0025422, 2022. doi: 10.1128/jcm.00254-22.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/56807
dc.identifier.issn.en_US.fl_str_mv 0095-1137
identifier_str_mv REZENDE, Izabela Maurício de et al. Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease. J Clin Microbiol., v. 60, n. 8, e0025422, 2022. doi: 10.1128/jcm.00254-22.
0095-1137
url https://www.arca.fiocruz.br/handle/icict/56807
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/56807/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/56807/2/Yellow%20Fever%20Molecular%20Diagnosis%20Using%20Urine%20Specimens%20during%20Acute.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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