Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies

Detalhes bibliográficos
Autor(a) principal: Carvalho, H. S.
Data de Publicação: 2005
Outros Autores: Baptista, M. L., Pinto, M. A., Silva, M., Takiya, C. M., Chagas, V., Pannain, V., Coelho, .H. S. M., Yoshida, C. F. T.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44555
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Carvalho, H. S.Baptista, M. L.Pinto, M. A.Silva, M.Takiya, C. M.Chagas, V.Pannain, V.Coelho, .H. S. M.Yoshida, C. F. T.2020-11-20T14:16:46Z2020-11-20T14:16:46Z2005CARVALHO, H. S. et al. Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies. memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 3, p. 269-272, May 2005.0074-0276https://www.arca.fiocruz.br/handle/icict/4455510.1590/S0074-027620050003000091678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Hepatite viralHibridização in situBiópsia hepáticaHepatite crônicaHepatitis C virus RNAHepatitis C virus RNAIn situ hybridizationLiver biopsyChronic hepatitisViral hepatitisDetection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Departamento de Histologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Centro de Ciências da Saúde. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Departamento de Clinica Médica, Setor de Gastroenterologia. Rio de Janeiro, RJ, Brasil. aFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fourteen hepatitis C virus (HCV) chronically infected patients were submitted to routine liver biopsy for histological evaluation. Liver samples were assayed to HCV-RNA by in situ hybridization, using digoxigenin labeled probe. HCV genotypes were found to be predominantly type 1 (71.4%), followed by genotype 3 (21.4%), and genotype 2 (7.2%). Alanine-aminotransferase levels were raised in 10 patients. The histopathological scores were minimal (21.4%), mild (57.2%), and moderate (21.4%). Viral RNA was detected in liver cells from nine patients (64.3%). ISH method provides localization and poor confirmation of HCV RNA in the liver tissue of HCV chronic patients.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44555/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHSCarvalho_etal_IOC_2005.pdfHSCarvalho_etal_IOC_2005.pdfapplication/pdf4113476https://www.arca.fiocruz.br/bitstream/icict/44555/2/HSCarvalho_etal_IOC_2005.pdf1b5236594a49376137fb1f3c0efbf7d4MD52TEXTHSCarvalho_etal_IOC_2005.pdf.txtHSCarvalho_etal_IOC_2005.pdf.txtExtracted texttext/plain21214https://www.arca.fiocruz.br/bitstream/icict/44555/3/HSCarvalho_etal_IOC_2005.pdf.txt63c908941dc7e45ab5fffcb1be5718ebMD53icict/445552020-11-21 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dc.title.pt_BR.fl_str_mv Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
title Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
spellingShingle Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
Carvalho, H. S.
Hepatite viral
Hibridização in situ
Biópsia hepática
Hepatite crônica
Hepatitis C virus RNA
Hepatitis C virus RNA
In situ hybridization
Liver biopsy
Chronic hepatitis
Viral hepatitis
title_short Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
title_full Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
title_fullStr Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
title_full_unstemmed Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
title_sort Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies
author Carvalho, H. S.
author_facet Carvalho, H. S.
Baptista, M. L.
Pinto, M. A.
Silva, M.
Takiya, C. M.
Chagas, V.
Pannain, V.
Coelho, .H. S. M.
Yoshida, C. F. T.
author_role author
author2 Baptista, M. L.
Pinto, M. A.
Silva, M.
Takiya, C. M.
Chagas, V.
Pannain, V.
Coelho, .H. S. M.
Yoshida, C. F. T.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, H. S.
Baptista, M. L.
Pinto, M. A.
Silva, M.
Takiya, C. M.
Chagas, V.
Pannain, V.
Coelho, .H. S. M.
Yoshida, C. F. T.
dc.subject.other.pt_BR.fl_str_mv Hepatite viral
Hibridização in situ
Biópsia hepática
Hepatite crônica
Hepatitis C virus RNA
topic Hepatite viral
Hibridização in situ
Biópsia hepática
Hepatite crônica
Hepatitis C virus RNA
Hepatitis C virus RNA
In situ hybridization
Liver biopsy
Chronic hepatitis
Viral hepatitis
dc.subject.en.pt_BR.fl_str_mv Hepatitis C virus RNA
In situ hybridization
Liver biopsy
Chronic hepatitis
Viral hepatitis
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2020-11-20T14:16:46Z
dc.date.available.fl_str_mv 2020-11-20T14:16:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, H. S. et al. Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies. memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 3, p. 269-272, May 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44555
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762005000300009
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv CARVALHO, H. S. et al. Detection of hepatitis C virus RNA by in situ hybridization in paraformaldehyde fixed biopsies. memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 3, p. 269-272, May 2005.
0074-0276
10.1590/S0074-02762005000300009
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44555
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44555/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/44555/2/HSCarvalho_etal_IOC_2005.pdf
https://www.arca.fiocruz.br/bitstream/icict/44555/3/HSCarvalho_etal_IOC_2005.pdf.txt
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