Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24942 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Marcon, Bruna HilzendegerHoletz, Fabíola BarbieriEastman, GuillermoOriga-Alves, Ana CarolinaAmorós, Mariana AndreaAguiar, Alessandra Melo deRebelatto, Carmen Lúcia KuniyoshiBrofman, Paulo Roberto SludSotelo-Silveira, JoseDallagiovanna, Bruno2018-02-26T14:50:50Z2018-02-26T14:50:50Z2017MARCON, Bruna H. et al. Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells. Stem Cell Research, n. 25, p. 191-201, 2017.1876-7753https://www.arca.fiocruz.br/handle/icict/2494210.1016/j.scr.2017.10.02710.1016/j.scr.2017.10.027porElsevierCélulas Estaminais Mesenquimais derivadas de AdiposeAdult Stem CellsAdipogenesisRibosomesProtein BiosynthesisMesenchymal Stromal CellsCélulas Madre AdultasAdipogénesisRibosomasBiosíntesis de ProteínasCélulas del Estroma MesenquimatosoCélulas-Tronco AdultasAdipogeniaRibossomosBiossíntese de ProteínasCélulas Mesenquimais EstromaisDownregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto de Investigaciones Biológicas Clemente Estable, Department of Genomics. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Institute of Biology and Experimental Medicine — National Council of Scientific and Technical Research (IByME-CONICET). Laboratory of Stem Cells. Ciudad Autónoma de Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Instituto de Investigaciones Biológicas Clemente Estable, Department of Genomics. Montevideo, Uruguay / Universidad de la Repúblic. School of Sciences. Department of Cell and Molecular Biology. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Commitment of adult stem cells involves the activation of specific gene networks regulated from transcription to protein synthesis. Here, we used ribosome profiling to identify mRNAs regulated at the translational level, through both differential association to polysomes and modulation of their translational rates. We observed that translational regulation during the differentiation of human adipose-derived stromal cells (hASCs, also known as adipose-derived mesenchymal stem cells), a subset of which are stem cells, to adipocytes was a major regulatory event. hASCs showed a significant reduction of whole protein synthesis after adipogenic induction and a downregulation of the expression and translational efficiency of ribosomal proteins. Additionally, focal adhesion and cytoskeletal proteins were downregulated at the translational level. This negative regulation of the essential biological functions of hASCs resulted in a reduction in cell size and the potential of hASCs to migrate. We analyzed whether the inactivation of key translation initiation factors was involved in this observed major repression of translation. We showed that there was an increase in the hypo phosphorylated forms of 4E-BP1, a negative regulator of translation, during early adipogenesis. Our results showed that extensive translational regulation occurred during the early stage of the adipogenic differentiation of hASCs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/24942/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALAguiarDallagiovanaDown.pdfAguiarDallagiovanaDown.pdfapplication/pdf1655163https://www.arca.fiocruz.br/bitstream/icict/24942/2/AguiarDallagiovanaDown.pdfa12594bf616019d421501a6a7f5a49ffMD52TEXTAguiarDallagiovanaDown.pdf.txtAguiarDallagiovanaDown.pdf.txtExtracted texttext/plain67069https://www.arca.fiocruz.br/bitstream/icict/24942/3/AguiarDallagiovanaDown.pdf.txtbca67b271d5f5014c232075236182143MD53icict/249422019-11-23 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| dc.title.pt_BR.fl_str_mv |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| title |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| spellingShingle |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells Marcon, Bruna Hilzendeger Células Estaminais Mesenquimais derivadas de Adipose Adult Stem Cells Adipogenesis Ribosomes Protein Biosynthesis Mesenchymal Stromal Cells Células Madre Adultas Adipogénesis Ribosomas Biosíntesis de Proteínas Células del Estroma Mesenquimatoso Células-Tronco Adultas Adipogenia Ribossomos Biossíntese de Proteínas Células Mesenquimais Estromais |
| title_short |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| title_full |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| title_fullStr |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| title_full_unstemmed |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| title_sort |
Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells |
| author |
Marcon, Bruna Hilzendeger |
| author_facet |
Marcon, Bruna Hilzendeger Holetz, Fabíola Barbieri Eastman, Guillermo Origa-Alves, Ana Carolina Amorós, Mariana Andrea Aguiar, Alessandra Melo de Rebelatto, Carmen Lúcia Kuniyoshi Brofman, Paulo Roberto Slud Sotelo-Silveira, Jose Dallagiovanna, Bruno |
| author_role |
author |
| author2 |
Holetz, Fabíola Barbieri Eastman, Guillermo Origa-Alves, Ana Carolina Amorós, Mariana Andrea Aguiar, Alessandra Melo de Rebelatto, Carmen Lúcia Kuniyoshi Brofman, Paulo Roberto Slud Sotelo-Silveira, Jose Dallagiovanna, Bruno |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Marcon, Bruna Hilzendeger Holetz, Fabíola Barbieri Eastman, Guillermo Origa-Alves, Ana Carolina Amorós, Mariana Andrea Aguiar, Alessandra Melo de Rebelatto, Carmen Lúcia Kuniyoshi Brofman, Paulo Roberto Slud Sotelo-Silveira, Jose Dallagiovanna, Bruno |
| dc.subject.other.pt_BR.fl_str_mv |
Células Estaminais Mesenquimais derivadas de Adipose |
| topic |
Células Estaminais Mesenquimais derivadas de Adipose Adult Stem Cells Adipogenesis Ribosomes Protein Biosynthesis Mesenchymal Stromal Cells Células Madre Adultas Adipogénesis Ribosomas Biosíntesis de Proteínas Células del Estroma Mesenquimatoso Células-Tronco Adultas Adipogenia Ribossomos Biossíntese de Proteínas Células Mesenquimais Estromais |
| dc.subject.en.pt_BR.fl_str_mv |
Adult Stem Cells Adipogenesis Ribosomes Protein Biosynthesis Mesenchymal Stromal Cells |
| dc.subject.es.pt_BR.fl_str_mv |
Células Madre Adultas Adipogénesis Ribosomas Biosíntesis de Proteínas Células del Estroma Mesenquimatoso |
| dc.subject.decs.pt_BR.fl_str_mv |
Células-Tronco Adultas Adipogenia Ribossomos Biossíntese de Proteínas Células Mesenquimais Estromais |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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2017 |
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2017 |
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2018-02-26T14:50:50Z |
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2018-02-26T14:50:50Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MARCON, Bruna H. et al. Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells. Stem Cell Research, n. 25, p. 191-201, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24942 |
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1876-7753 |
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10.1016/j.scr.2017.10.027 |
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10.1016/j.scr.2017.10.027 |
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MARCON, Bruna H. et al. Downregulation of the protein synthesis machinery is a major regulatory event during early adipogenic differentiation of human adipose-derived stromal cells. Stem Cell Research, n. 25, p. 191-201, 2017. 1876-7753 10.1016/j.scr.2017.10.027 |
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Elsevier |
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Elsevier |
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