Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes

Detalhes bibliográficos
Autor(a) principal: Inoue, Alexandre Haruo
Data de Publicação: 2022
Outros Autores: Domingues, Patricia Ferreira, Serpeloni, Mariana, Hiraiwa, Priscila Mazzocchi, Vidal, Newton Medeiros, Butterfield, Erin R., Pino, Ricardo Canavate del, Ludwig, Adriana, Boehm, Cordula, Field, Mark C., Ávila, Andréa Rodrigues
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52506
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Inoue, Alexandre HaruoDomingues, Patricia FerreiraSerpeloni, MarianaHiraiwa, Priscila MazzocchiVidal, Newton MedeirosButterfield, Erin R.Pino, Ricardo Canavate delLudwig, AdrianaBoehm, CordulaField, Mark C.Ávila, Andréa Rodrigues2022-05-02T18:59:08Z2022-05-02T18:59:08Z2022INOUE, Alexandre Haruo et al. Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes. MCP Research, v. 21 n. 3, p. 1–24, 2022.1535-9484https://www.arca.fiocruz.br/handle/icict/5250610.1016/j.mcpro.2022.100208porAmerican Society for Biochemistry and Molecular Biology / ElsevierTrypanosoma cruziTrypanosomaCinetoplastídeosProteomicsRNA, MessengerRNA SplicingProteómicaARN MensajeroEmpalme del ARNProtéomiqueARN messagerÉpissage des ARNProteômicaRNA MensageiroSplicing de RNAProteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.National Center for Biotechnology Information. National Library of Medicine. National Institutes of Health. Bethesda, Maryland, USA.School of Life Sciences. University of Dundee. Dundee, Scotland, UK.School of Life Sciences. University of Dundee. Dundee, Scotland, UK.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.School of Life Sciences. University of Dundee. Dundee, Scotland, UK.School of Life Sciences. University of Dundee. Dundee, Scotland, UK. / Biology Centre. University of South Bohemia. Ceské Budejovice, Czech Republic.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.In trypanosomatids, transcription is polycistronic and all mRNAs are processed by trans-splicing, with export mediated by noncanonical mechanisms. Although mRNA export is central to gene regulation and expression, few orthologs of proteins involved in mRNA export in higher eukaryotes are detectable in trypanosome genomes, necessitating direct identification of protein components. We previously described conserved mRNA export pathway components in Trypanosoma cruzi, including orthologs of Sub2, a component of the TREX complex, and eIF4AIII (previously Hel45), a core component of the exon junction complex (EJC). Here, we searched for protein interactors of both proteins using cryomilling and mass spectrometry. Significant overlap between TcSub2 and TceIF4AIII-interacting protein cohorts suggests that both proteins associate with similar machinery. We identified several interactions with conserved core components of the EJC and multiple additional complexes, together with proteins specific to trypanosomatids. Additional immunoisolations of kinetoplastid-specific proteins both validated and extended the superinteractome, which is capable of supporting RNA processing from splicing through to nuclear export and cytoplasmic events. We also suggest that only proteomics is powerful enough to uncover the high connectivity between multiple aspects of mRNA metabolism and to uncover kinetoplastid-specific components that create a unique amalgam to support trypanosome mRNA maturation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/52506/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALmain2OK.pdfmain2OK.pdfapplication/pdf4033559https://www.arca.fiocruz.br/bitstream/icict/52506/2/main2OK.pdff52d90e20fdb3d9ba0f9617d4fa6d5e6MD52icict/525062022-05-02 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dc.title.pt_BR.fl_str_mv Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
title Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
spellingShingle Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
Inoue, Alexandre Haruo
Trypanosoma cruzi
Trypanosoma
Cinetoplastídeos
Proteomics
RNA, Messenger
RNA Splicing
Proteómica
ARN Mensajero
Empalme del ARN
Protéomique
ARN messager
Épissage des ARN
Proteômica
RNA Mensageiro
Splicing de RNA
title_short Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
title_full Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
title_fullStr Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
title_full_unstemmed Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
title_sort Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes
author Inoue, Alexandre Haruo
author_facet Inoue, Alexandre Haruo
Domingues, Patricia Ferreira
Serpeloni, Mariana
Hiraiwa, Priscila Mazzocchi
Vidal, Newton Medeiros
Butterfield, Erin R.
Pino, Ricardo Canavate del
Ludwig, Adriana
Boehm, Cordula
Field, Mark C.
Ávila, Andréa Rodrigues
author_role author
author2 Domingues, Patricia Ferreira
Serpeloni, Mariana
Hiraiwa, Priscila Mazzocchi
Vidal, Newton Medeiros
Butterfield, Erin R.
Pino, Ricardo Canavate del
Ludwig, Adriana
Boehm, Cordula
Field, Mark C.
Ávila, Andréa Rodrigues
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Inoue, Alexandre Haruo
Domingues, Patricia Ferreira
Serpeloni, Mariana
Hiraiwa, Priscila Mazzocchi
Vidal, Newton Medeiros
Butterfield, Erin R.
Pino, Ricardo Canavate del
Ludwig, Adriana
Boehm, Cordula
Field, Mark C.
Ávila, Andréa Rodrigues
dc.subject.other.pt_BR.fl_str_mv Trypanosoma cruzi
Trypanosoma
Cinetoplastídeos
topic Trypanosoma cruzi
Trypanosoma
Cinetoplastídeos
Proteomics
RNA, Messenger
RNA Splicing
Proteómica
ARN Mensajero
Empalme del ARN
Protéomique
ARN messager
Épissage des ARN
Proteômica
RNA Mensageiro
Splicing de RNA
dc.subject.en.pt_BR.fl_str_mv Proteomics
RNA, Messenger
RNA Splicing
dc.subject.es.pt_BR.fl_str_mv Proteómica
ARN Mensajero
Empalme del ARN
dc.subject.fr.pt_BR.fl_str_mv Protéomique
ARN messager
Épissage des ARN
dc.subject.decs.pt_BR.fl_str_mv Proteômica
RNA Mensageiro
Splicing de RNA
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-05-02T18:59:08Z
dc.date.available.fl_str_mv 2022-05-02T18:59:08Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv INOUE, Alexandre Haruo et al. Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes. MCP Research, v. 21 n. 3, p. 1–24, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52506
dc.identifier.issn.pt_BR.fl_str_mv 1535-9484
dc.identifier.doi.none.fl_str_mv 10.1016/j.mcpro.2022.100208
identifier_str_mv INOUE, Alexandre Haruo et al. Proteomics uncovers novel components of na interactive protein network supporting RNA export in trypanosomes. MCP Research, v. 21 n. 3, p. 1–24, 2022.
1535-9484
10.1016/j.mcpro.2022.100208
url https://www.arca.fiocruz.br/handle/icict/52506
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology / Elsevier
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology / Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/52506/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/52506/2/main2OK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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