Alternative methods as tools for obesity research: In vitro and in silico approaches
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57379 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
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Pamplona, Juliana HelenaZoehler, BernardoShigunov, PatríciaBarisón, María JuliaSevero, Vanessa RossiniErich, Natalie MayaraRibeiro, Annanda LyraHorinouchi, Cintia Delai da SilvaSuzukawa, Andréia AkemiRobert, Anny WaloskiAbud, Ana Paula RessettiAguiar, Alessandra Melo de2023-03-15T14:14:35Z2023-03-15T14:14:35Z2023PAMPLONA, Juliana Helena et al. Alternative methods as tools for obesity research: In vitro and in silico approaches. Life, v. 13, n. 108, p. 1–25, 2023.2075-1729https://www.arca.fiocruz.br/handle/icict/5737910.3390/life13010108porMDPIAdipogeniaMétodos Alternativos in SilicoCultura Celular 3DObesityAdipogenesisMesenchymal Stem CellsAdipose TissueIn Vitro TechniquesCell Culture Techniques, Three DimensionalMicrofluidicsObesidadAdipogénesisCélulas Madre MesenquimatosasTejido AdiposoTécnicas In VitroTécnicas de Cultivo Tridimensional de CélulasMicrofluídicaObésitéAdipogenèseCellules souches mésenchymateusesTissu adipeuxTechniques in vitroMicrofluidiqueObesidadeAdipogêneseCélulas-Tronco MesenquimaisTecido AdiposoTécnicas In VitroTécnicas de Cultura de Células em Três DimensõesMicrofluídicaAlternative methods as tools for obesity research: In vitro and in silico approachesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Pesquisa em Apicomplexa. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil.The study of adipogenesis is essential for understanding and treating obesity, a multifactorial problem related to body fat accumulation that leads to several life-threatening diseases, becoming one of the most critical public health problems worldwide. In this review, we propose to provide the highlights of the adipogenesis study based on in vitro differentiation of human mesenchymal stem cells (hMSCs). We list in silico methods, such as molecular docking for identification of molecular targets, and in vitro approaches, from 2D, more straightforward and applied for screening large libraries of substances, to more representative physiological models, such as 3D and bioprinting models. We also describe the development of physiological models based on microfluidic systems applied to investigate adipogenesis in vitro. We intend to identify the main alternative models for adipogenesis evaluation, contributing to the direction of preclinical research in obesity. Future directions indicate the association of in silico and in vitro techniques to bring a clear picture of alternative methods based on adipogenesis as a tool for obesity research.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALlife-13-00108.pdflife-13-00108.pdfapplication/pdf5298928https://www.arca.fiocruz.br/bitstream/icict/57379/2/life-13-00108.pdfee3067b2ef6c30195c53516dff125328MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57379/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/573792023-03-15 11:14:36.725oai:www.arca.fiocruz.br:icict/57379Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T14:14:36Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| title |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| spellingShingle |
Alternative methods as tools for obesity research: In vitro and in silico approaches Pamplona, Juliana Helena Adipogenia Métodos Alternativos in Silico Cultura Celular 3D Obesity Adipogenesis Mesenchymal Stem Cells Adipose Tissue In Vitro Techniques Cell Culture Techniques, Three Dimensional Microfluidics Obesidad Adipogénesis Células Madre Mesenquimatosas Tejido Adiposo Técnicas In Vitro Técnicas de Cultivo Tridimensional de Células Microfluídica Obésité Adipogenèse Cellules souches mésenchymateuses Tissu adipeux Techniques in vitro Microfluidique Obesidade Adipogênese Células-Tronco Mesenquimais Tecido Adiposo Técnicas In Vitro Técnicas de Cultura de Células em Três Dimensões Microfluídica |
| title_short |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| title_full |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| title_fullStr |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| title_full_unstemmed |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| title_sort |
Alternative methods as tools for obesity research: In vitro and in silico approaches |
| author |
Pamplona, Juliana Helena |
| author_facet |
Pamplona, Juliana Helena Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti Aguiar, Alessandra Melo de |
| author_role |
author |
| author2 |
Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti Aguiar, Alessandra Melo de |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pamplona, Juliana Helena Zoehler, Bernardo Shigunov, Patrícia Barisón, María Julia Severo, Vanessa Rossini Erich, Natalie Mayara Ribeiro, Annanda Lyra Horinouchi, Cintia Delai da Silva Suzukawa, Andréia Akemi Robert, Anny Waloski Abud, Ana Paula Ressetti Aguiar, Alessandra Melo de |
| dc.subject.other.en_US.fl_str_mv |
Adipogenia Métodos Alternativos in Silico Cultura Celular 3D |
| topic |
Adipogenia Métodos Alternativos in Silico Cultura Celular 3D Obesity Adipogenesis Mesenchymal Stem Cells Adipose Tissue In Vitro Techniques Cell Culture Techniques, Three Dimensional Microfluidics Obesidad Adipogénesis Células Madre Mesenquimatosas Tejido Adiposo Técnicas In Vitro Técnicas de Cultivo Tridimensional de Células Microfluídica Obésité Adipogenèse Cellules souches mésenchymateuses Tissu adipeux Techniques in vitro Microfluidique Obesidade Adipogênese Células-Tronco Mesenquimais Tecido Adiposo Técnicas In Vitro Técnicas de Cultura de Células em Três Dimensões Microfluídica |
| dc.subject.en.en_US.fl_str_mv |
Obesity Adipogenesis Mesenchymal Stem Cells Adipose Tissue In Vitro Techniques Cell Culture Techniques, Three Dimensional Microfluidics |
| dc.subject.es.en_US.fl_str_mv |
Obesidad Adipogénesis Células Madre Mesenquimatosas Tejido Adiposo Técnicas In Vitro Técnicas de Cultivo Tridimensional de Células Microfluídica |
| dc.subject.fr.en_US.fl_str_mv |
Obésité Adipogenèse Cellules souches mésenchymateuses Tissu adipeux Techniques in vitro Microfluidique |
| dc.subject.decs.en_US.fl_str_mv |
Obesidade Adipogênese Células-Tronco Mesenquimais Tecido Adiposo Técnicas In Vitro Técnicas de Cultura de Células em Três Dimensões Microfluídica |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
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2023 |
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2023-03-15T14:14:35Z |
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2023-03-15T14:14:35Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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PAMPLONA, Juliana Helena et al. Alternative methods as tools for obesity research: In vitro and in silico approaches. Life, v. 13, n. 108, p. 1–25, 2023. |
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https://www.arca.fiocruz.br/handle/icict/57379 |
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2075-1729 |
| dc.identifier.doi.none.fl_str_mv |
10.3390/life13010108 |
| identifier_str_mv |
PAMPLONA, Juliana Helena et al. Alternative methods as tools for obesity research: In vitro and in silico approaches. Life, v. 13, n. 108, p. 1–25, 2023. 2075-1729 10.3390/life13010108 |
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MDPI |
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MDPI |
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