Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells

Detalhes bibliográficos
Autor(a) principal: Horinouchi, Cintia D. S.
Data de Publicação: 2020
Outros Autores: Barisón, María Julia, Robert, Anny Waloski, Kuligovski, Crisciele, Aguiar, Alessandra Melo de, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46082
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Horinouchi, Cintia D. S.Barisón, María JuliaRobert, Anny WaloskiKuligovski, CriscieleAguiar, Alessandra Melo deDallagiovanna, Bruno2021-02-15T16:06:37Z2021-02-15T16:06:37Z2020HORINOUCHI, Cintia D. S. et al. Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells. World Journal Stem Cells, v.12, n. 12, p. 1640-1651, 2020.1948-0210https://www.arca.fiocruz.br/handle/icict/4608210.4252/wjsc.v12.i12.1640porBaishideng Publishing GroupCélulas-Tronco MesenquimaisMesenchymal Stem CellsAdipogenesisOsteogenesisAge FactorsCélulas Madre MesenquimatosasAdipogénesisOsteogénesisFactores de EdadCellules souches mésenchymateusesAdipogenèseOstéogenèseFacteurs âgesAdipogêneseOsteogêneseDiferenciação CelularFatores EtáriosInfluence of donor age on the differentiation and division capacity of human adipose-derived stem cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Human adipose-derived stromal/stem cells (hASCs) are one of the most useful types of mesenchymal stromal/stem cells, which are adult multipotent cells with great therapeutic potential for the treatment of several diseases. However, for successful clinical application, it is critical that high-quality cells can be obtained. Diverse factors seem to be able to influence cell quality and performance, especially factors related to donors’ intrinsic characteristics, such as age. Nevertheless, there is no consensus regarding this characteristic, and there is conflicting information in the literature. To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors. hASCs were harvested from liposuctioned adipose tissue obtained from female donors (aged 20-70 years). Cells were distributed into two groups according to age range: old hASCs (oASCs, ≥ 55 years, n = 9) and young hASCs (yASCs, ≤ 35 years, n = 9). For each group, immunophenotypic characterization was performed by flow cytometry. Population doubling time was assessed over seven days. For adipogenic potential evaluation, lipid deposits were assessed after 7 d, 14 d and 21 d of adipogenic induction. Osteogenic potential was verified by analyzing cell mineralization after 14 d, 21 d and 28 d of osteogenic induction. mRNA expression of PPARγ2, CEBPA and Runx hASCs were successfully obtained, cultured, and grouped according to their age: yASCs (26.33 ± 4.66 years old) and oASCs (64.78 ± 4.58 years old). After maintenance of the cells in culture, there were no differences in morphology between cells from the young and old donors. Additionally, both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells. The average doubling time indicated that yASCs (4.09 ± 0.94 d) did not significantly differ from oASCs (4.19 ± 1.29 d). Concerning differentiation potential, after adipogenic and osteogenic induction, yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells. However, no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis. Additionally, the mRNA expression of PPARγ2, CEBPA and Runx2 were similar in yASCs and oASCs. Our findings suggest that age does not seem to significantly affect the cell Division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/46082/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALWJSC-12-1640ok.pdfWJSC-12-1640ok.pdfapplication/pdf8575166https://www.arca.fiocruz.br/bitstream/icict/46082/2/WJSC-12-1640ok.pdf98afb5b7382d34df224dd73d18c1f56bMD52TEXTWJSC-12-1640ok.pdf.txtWJSC-12-1640ok.pdf.txtExtracted texttext/plain45210https://www.arca.fiocruz.br/bitstream/icict/46082/3/WJSC-12-1640ok.pdf.txt8caa42816cd9e0e0434feca754c41893MD53icict/460822021-02-22 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dc.title.pt_BR.fl_str_mv Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
title Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
spellingShingle Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
Horinouchi, Cintia D. S.
Células-Tronco Mesenquimais
Mesenchymal Stem Cells
Adipogenesis
Osteogenesis
Age Factors
Células Madre Mesenquimatosas
Adipogénesis
Osteogénesis
Factores de Edad
Cellules souches mésenchymateuses
Adipogenèse
Ostéogenèse
Facteurs âges
Adipogênese
Osteogênese
Diferenciação Celular
Fatores Etários
title_short Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
title_full Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
title_fullStr Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
title_full_unstemmed Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
title_sort Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
author Horinouchi, Cintia D. S.
author_facet Horinouchi, Cintia D. S.
Barisón, María Julia
Robert, Anny Waloski
Kuligovski, Crisciele
Aguiar, Alessandra Melo de
Dallagiovanna, Bruno
author_role author
author2 Barisón, María Julia
Robert, Anny Waloski
Kuligovski, Crisciele
Aguiar, Alessandra Melo de
Dallagiovanna, Bruno
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Horinouchi, Cintia D. S.
Barisón, María Julia
Robert, Anny Waloski
Kuligovski, Crisciele
Aguiar, Alessandra Melo de
Dallagiovanna, Bruno
dc.subject.other.pt_BR.fl_str_mv Células-Tronco Mesenquimais
topic Células-Tronco Mesenquimais
Mesenchymal Stem Cells
Adipogenesis
Osteogenesis
Age Factors
Células Madre Mesenquimatosas
Adipogénesis
Osteogénesis
Factores de Edad
Cellules souches mésenchymateuses
Adipogenèse
Ostéogenèse
Facteurs âges
Adipogênese
Osteogênese
Diferenciação Celular
Fatores Etários
dc.subject.en.pt_BR.fl_str_mv Mesenchymal Stem Cells
Adipogenesis
Osteogenesis
Age Factors
dc.subject.es.pt_BR.fl_str_mv Células Madre Mesenquimatosas
Adipogénesis
Osteogénesis
Factores de Edad
dc.subject.fr.pt_BR.fl_str_mv Cellules souches mésenchymateuses
Adipogenèse
Ostéogenèse
Facteurs âges
dc.subject.decs.pt_BR.fl_str_mv Adipogênese
Osteogênese
Diferenciação Celular
Fatores Etários
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-02-15T16:06:37Z
dc.date.available.fl_str_mv 2021-02-15T16:06:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HORINOUCHI, Cintia D. S. et al. Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells. World Journal Stem Cells, v.12, n. 12, p. 1640-1651, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46082
dc.identifier.issn.pt_BR.fl_str_mv 1948-0210
dc.identifier.doi.none.fl_str_mv 10.4252/wjsc.v12.i12.1640
identifier_str_mv HORINOUCHI, Cintia D. S. et al. Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells. World Journal Stem Cells, v.12, n. 12, p. 1640-1651, 2020.
1948-0210
10.4252/wjsc.v12.i12.1640
url https://www.arca.fiocruz.br/handle/icict/46082
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Baishideng Publishing Group
publisher.none.fl_str_mv Baishideng Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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