Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction

Detalhes bibliográficos
Autor(a) principal: Abud, Ana Paula Ressetti
Data de Publicação: 2021
Outros Autores: Paschoal, Ariane Caroline Campos, Kuligovski, Crisciele, Caruso, Rodrigo Rêgo Barros, Dallagiovanna, Bruno, Aguiar, Alessandra Melo de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50405
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil.
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spelling Abud, Ana Paula RessettiPaschoal, Ariane Caroline CamposKuligovski, CriscieleCaruso, Rodrigo Rêgo BarrosDallagiovanna, BrunoAguiar, Alessandra Melo de2021-12-14T13:28:59Z2021-12-14T13:28:59Z2021ABUD, Ana Paula Ressetti et al. Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction. MethodsX, v.8, n. 101515, p. 1–22, 2021.2215-0161https://www.arca.fiocruz.br/handle/icict/5040510.1016/j.mex.2021.101515porElsevierUsing inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity predictioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. / Grupo Boticário. Pesquisa and Desenvolvimento. São José dos Pinhais, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.In vitro stem cell models are used as alternatives to animal models and are important tools for cytotoxicity studies. Researchers can determine the effects of test substances on human cells by evaluating cell viability and differentiation. Here, we describe an in vitro model to quantify adipogenesis based on the Nile red staining of specific lipid droplets and the emission of basic lipids from human adipose tissue-derived mesenchymal stromal cells (AD-MSCs) in the presence of test substances. This assay allows for the prediction of toxicity based on the inhibition of adipogenesis in vitro in a 96-well format. The differentiation of a progenitor cell into a specialized cell, the adipocyte, is easy to monitor and quantify, making this a simple assay. The fluorescence staining of nuclei and lipid droplets is measured after 14 days of cell differentiation to determine cell number and assess cell differentiation using high-content imaging analysis, thus allowing for the identification of chemicals that impact differentiation. We also describe a protocol to assess adipocyte differentiation by fluorescence intensity using a multiplate reader.Stem CellsCell DifferentiationCytotoxicityAdipogenesisCélulas MadreDiferenciación CelularCitotoxicidadAdipogénesisCellules souchesDifférenciation cellulaireCytotoxicitéAdipogenèseCélulas-TroncoDiferenciação CelularCitotoxicidadeAdipogeniainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/50405/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1-s2.0-S2215016121003083OK.pdf1-s2.0-S2215016121003083OK.pdfapplication/pdf3357165https://www.arca.fiocruz.br/bitstream/icict/50405/2/1-s2.0-S2215016121003083OK.pdf08e485666c223cf6560c4cb1098d6818MD52icict/504052021-12-14 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dc.title.pt_BR.fl_str_mv Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
title Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
spellingShingle Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
Abud, Ana Paula Ressetti
Stem Cells
Cell Differentiation
Cytotoxicity
Adipogenesis
Células Madre
Diferenciación Celular
Citotoxicidad
Adipogénesis
Cellules souches
Différenciation cellulaire
Cytotoxicité
Adipogenèse
Células-Tronco
Diferenciação Celular
Citotoxicidade
Adipogenia
title_short Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
title_full Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
title_fullStr Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
title_full_unstemmed Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
title_sort Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction
author Abud, Ana Paula Ressetti
author_facet Abud, Ana Paula Ressetti
Paschoal, Ariane Caroline Campos
Kuligovski, Crisciele
Caruso, Rodrigo Rêgo Barros
Dallagiovanna, Bruno
Aguiar, Alessandra Melo de
author_role author
author2 Paschoal, Ariane Caroline Campos
Kuligovski, Crisciele
Caruso, Rodrigo Rêgo Barros
Dallagiovanna, Bruno
Aguiar, Alessandra Melo de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Abud, Ana Paula Ressetti
Paschoal, Ariane Caroline Campos
Kuligovski, Crisciele
Caruso, Rodrigo Rêgo Barros
Dallagiovanna, Bruno
Aguiar, Alessandra Melo de
dc.subject.en.pt_BR.fl_str_mv Stem Cells
Cell Differentiation
Cytotoxicity
Adipogenesis
topic Stem Cells
Cell Differentiation
Cytotoxicity
Adipogenesis
Células Madre
Diferenciación Celular
Citotoxicidad
Adipogénesis
Cellules souches
Différenciation cellulaire
Cytotoxicité
Adipogenèse
Células-Tronco
Diferenciação Celular
Citotoxicidade
Adipogenia
dc.subject.es.pt_BR.fl_str_mv Células Madre
Diferenciación Celular
Citotoxicidad
Adipogénesis
dc.subject.fr.pt_BR.fl_str_mv Cellules souches
Différenciation cellulaire
Cytotoxicité
Adipogenèse
dc.subject.decs.pt_BR.fl_str_mv Células-Tronco
Diferenciação Celular
Citotoxicidade
Adipogenia
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Rede de Plataformas Tecnológicas FIOCRUZ – Bioensaios com Métodos Alternativos em Citotoxicidade. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-12-14T13:28:59Z
dc.date.available.fl_str_mv 2021-12-14T13:28:59Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ABUD, Ana Paula Ressetti et al. Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction. MethodsX, v.8, n. 101515, p. 1–22, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50405
dc.identifier.issn.pt_BR.fl_str_mv 2215-0161
dc.identifier.doi.none.fl_str_mv 10.1016/j.mex.2021.101515
identifier_str_mv ABUD, Ana Paula Ressetti et al. Using inhibition of the adipogenesis of adipose-derived stem cells in vitro for toxicity prediction. MethodsX, v.8, n. 101515, p. 1–22, 2021.
2215-0161
10.1016/j.mex.2021.101515
url https://www.arca.fiocruz.br/handle/icict/50405
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50405/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50405/2/1-s2.0-S2215016121003083OK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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