Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45943 |
Resumo: | Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brazil. |
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Queiroz, Jackson Alves da SilvaRampazzo, Rita de Cássia PontelloSilva Filho, Edivá Basílio daOliveira, Gabriella SgorlonOliveira, Suyane da CostaSouza, Luan Felipo BotelhoPereira, Soraya dos SantosRodrigues, Moreno Magalhães de SouzaMaia, Adriana Cristina SalvadorSilva, Cicileia Correia daMendonça, Aline Linhares Ferreira de MeloLugtenburg, Celina Aparecida BertoniAguiar, Francisco de Assis AraújoRodrigues, Rosiane de Souza SoaresSantos, Caio Henrique NemethGuimarães, Alice Paula Di SabatinoMáximo, Fernando RodriguesSantos, Alcione de Oliveira dosKrieger, Marco AurélioSalcedo, Juan Miguel VillalobosDall’Acqua, Deusilene Souza Vieira2021-02-03T13:49:56Z2021-02-03T13:49:56Z2021QUEIROZ, Jackson Alves da Silva et al. Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix. International Journal of Infectious Diseases, p. 1-21, 1 Jan. 2021.1201-9712https://www.arca.fiocruz.br/handle/icict/4594310.1016/j.ijid.2021.01.001engElsevierhttps://www.arca.fiocruz.br/handle/icict/46315Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brazil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Laboratório Central de Saúde Pública do Estado de Rondônia. Porto Velho, RO, Brazil.Secretaria de Estado de Finanças de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil.Secretaria de Estado da Saúde de Rondônia. Porto Velho, RO, Brasil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brazil.Introduction: COVID-19 is a disease caused by Severe Acute Respiratory Syndrome Virus 2 (SARS-CoV-2) which emerged in China in late 2019. The rapid viral spread has made the disease a public health emergency of worldwide concern. The gold standard for diagnosing SARS-CoV-2 is reverse transcription followed by qualitative real-time polymerase chain reaction (RT-qPCR); however, the role of viral load quantification has not been thoroughly investigated yet. Objective: The aim of this study was to develop a high-precision quantitative one-step RT-qPCR reaction using the association of the viral target and the human target in the same reaction. Methods: The assay standardization involved the absolute quantification method with serial dilutions of a plasmid with the N gene in a biological matrix to build a standard curve. Results and Discussion: The results demonstrated the possibility of quantifying as few as 2.5 copies/reaction and analysis of 244 patients with known results selected by cross- section revealed 100% agreement with a qualitative RT-qPCR assay registered by Anvisa. In this population, it was possible to quantify patients with between 2.59 and 3.5x10⁷ copies per reaction and negative patients continued to indicate the same result. Conclusion: This assay can be a useful tool for the proper patients management, since the level and duration of viral replication are important factors to assess the risk of transmission and to guide decisions regarding the isolation and release of patients also, an accurate diagnosis is critical information whereas the current COVID-19 pandemic represents the biggest current global health problem.SARS-CoV-2Coronavirus InfectionsReal-Time Polymerase Chain ReactionReagent Kits DiagnosticCOVID-19info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| title |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| spellingShingle |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) Queiroz, Jackson Alves da Silva SARS-CoV-2 Coronavirus Infections Real-Time Polymerase Chain Reaction Reagent Kits Diagnostic COVID-19 |
| title_short |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| title_full |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| title_fullStr |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| title_full_unstemmed |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| title_sort |
Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix (preprint) |
| author |
Queiroz, Jackson Alves da Silva |
| author_facet |
Queiroz, Jackson Alves da Silva Rampazzo, Rita de Cássia Pontello Silva Filho, Edivá Basílio da Oliveira, Gabriella Sgorlon Oliveira, Suyane da Costa Souza, Luan Felipo Botelho Pereira, Soraya dos Santos Rodrigues, Moreno Magalhães de Souza Maia, Adriana Cristina Salvador Silva, Cicileia Correia da Mendonça, Aline Linhares Ferreira de Melo Lugtenburg, Celina Aparecida Bertoni Aguiar, Francisco de Assis Araújo Rodrigues, Rosiane de Souza Soares Santos, Caio Henrique Nemeth Guimarães, Alice Paula Di Sabatino Máximo, Fernando Rodrigues Santos, Alcione de Oliveira dos Krieger, Marco Aurélio Salcedo, Juan Miguel Villalobos Dall’Acqua, Deusilene Souza Vieira |
| author_role |
author |
| author2 |
Rampazzo, Rita de Cássia Pontello Silva Filho, Edivá Basílio da Oliveira, Gabriella Sgorlon Oliveira, Suyane da Costa Souza, Luan Felipo Botelho Pereira, Soraya dos Santos Rodrigues, Moreno Magalhães de Souza Maia, Adriana Cristina Salvador Silva, Cicileia Correia da Mendonça, Aline Linhares Ferreira de Melo Lugtenburg, Celina Aparecida Bertoni Aguiar, Francisco de Assis Araújo Rodrigues, Rosiane de Souza Soares Santos, Caio Henrique Nemeth Guimarães, Alice Paula Di Sabatino Máximo, Fernando Rodrigues Santos, Alcione de Oliveira dos Krieger, Marco Aurélio Salcedo, Juan Miguel Villalobos Dall’Acqua, Deusilene Souza Vieira |
| author2_role |
author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Queiroz, Jackson Alves da Silva Rampazzo, Rita de Cássia Pontello Silva Filho, Edivá Basílio da Oliveira, Gabriella Sgorlon Oliveira, Suyane da Costa Souza, Luan Felipo Botelho Pereira, Soraya dos Santos Rodrigues, Moreno Magalhães de Souza Maia, Adriana Cristina Salvador Silva, Cicileia Correia da Mendonça, Aline Linhares Ferreira de Melo Lugtenburg, Celina Aparecida Bertoni Aguiar, Francisco de Assis Araújo Rodrigues, Rosiane de Souza Soares Santos, Caio Henrique Nemeth Guimarães, Alice Paula Di Sabatino Máximo, Fernando Rodrigues Santos, Alcione de Oliveira dos Krieger, Marco Aurélio Salcedo, Juan Miguel Villalobos Dall’Acqua, Deusilene Souza Vieira |
| dc.subject.en.pt_BR.fl_str_mv |
SARS-CoV-2 Coronavirus Infections Real-Time Polymerase Chain Reaction Reagent Kits Diagnostic COVID-19 |
| topic |
SARS-CoV-2 Coronavirus Infections Real-Time Polymerase Chain Reaction Reagent Kits Diagnostic COVID-19 |
| description |
Fundação Oswaldo Cruz. Fiocruz Rondônia. Porto Velho, RO, Brazil / Fundação Universidade Federal de Rondônia. Programa de Pós Graduação em Biologia Experimental. Porto Velho, RO, Brazil. |
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2021 |
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2021-02-03T13:49:56Z |
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2021-02-03T13:49:56Z |
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2021 |
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info:eu-repo/semantics/article |
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publishedVersion |
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QUEIROZ, Jackson Alves da Silva et al. Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix. International Journal of Infectious Diseases, p. 1-21, 1 Jan. 2021. |
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https://www.arca.fiocruz.br/handle/icict/45943 |
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1201-9712 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1016/j.ijid.2021.01.001 |
| identifier_str_mv |
QUEIROZ, Jackson Alves da Silva et al. Development of a quantitative one-step multiplex RT-qPCR assay for the detection of SARS-CoV-2 in a biological matrix. International Journal of Infectious Diseases, p. 1-21, 1 Jan. 2021. 1201-9712 10.1016/j.ijid.2021.01.001 |
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Elsevier |
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Elsevier |
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