Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/15244 |
Resumo: | University of São Paulo. Institute of Biomedical Sciences. Department of Immunology. São Paulo, SP, Brasil |
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Castiblanco-Valencia, Mónica MFraga, Tatiana RodriguesBreda, Leandro Carvalho DantasVasconcellos, Sílvio ArrudaFigueira, Cláudio PereiraPicardeau, MathieuWunder Junior, Elsio AugustoKo, Albert IcksangBarbosa, Angela SilvaIsaac, Lourdes2016-08-11T16:49:53Z2016-08-11T16:49:53Z2016CASTIBLANCO-VALENCIA, M. M. et al. Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum. Immunology Letters, v. 173, p. 61–68, 2016.0165-2478https://www.arca.fiocruz.br/handle/icict/1524410.1016/j.imlet.2016.03.005engElsevierAcquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human seruminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of São Paulo. Institute of Biomedical Sciences. Department of Immunology. São Paulo, SP, BrasilUniversity of São Paulo. Institute of Biomedical Sciences. Department of Immunology. São Paulo, SP, BrasilUniversity of São Paulo. Institute of Biomedical Sciences. Department of Immunology. São Paulo, SP, BrasilUniversity of São Paulo. Faculty of Veterinary Medicine. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur. Biology of Spirochetes Unit. Paris, FranceeYale University. Department of Epidemiology of Microbial Diseases. USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Yale University. Department of Epidemiology of Microbial Diseases. USAButantan Institute. Laboratory of Bacteriology. São Paulo, SP, BrasilUniversity of São Paulo. Institute of Biomedical Sciences. Department of Immunology. São Paulo, SP, BrasilLeptospiral immunoglobulin-like (Lig) proteins are surface exposed molecules present in pathogenic but not in saprophytic Leptospira species. We have previously shown that Lig proteins interact with the soluble complement regulators Factor H (FH), FH like-1 (FHL-1), FH related-1 (FHR-1) and C4b Binding Protein (C4BP). In this study, we used the saprophyte L. biflexa serovar Patoc as a surrogate host to address the specific role of LigA and LigB proteins in leptospiral complement evasion. L. biflexa expressing LigA or LigB was able to acquire FH and C4BP. Bound complement regulators retained their cofactor activities of FI in the proteolytic cleavage of C3b and C4b. Moreover, heterologous expression of ligA and ligB genes in the saprophyte L. biflexa enhanced bacterial survival in human serum. Complement deposition on lig-transformed L. biflexa was assessed by flow cytometry analysis. With regard to MAC deposition, L. biflexa expressing LigA or LigB presented an intermediate profile: MAC deposition levels were greater than those found in the pathogenic L. interrogans, but lower than those observed for L. biflexa wildtype. In conclusion, Lig proteins contribute to in vitro control of complement activation on the leptospiral surface, promoting an increased bacterial survival in human serum.LeptospiraComplementImmune evasionLig proteinsFactor HC4BPinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15244/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALValencia MMC Acquisition of negative.pdfValencia MMC Acquisition of negative.pdfapplication/pdf2201621https://www.arca.fiocruz.br/bitstream/icict/15244/2/Valencia%20MMC%20Acquisition%20of%20negative.pdf256fd22844511b0f8dd7550132f2cf45MD52TEXTValencia MMC Acquisition of negative.pdf.txtValencia MMC Acquisition of negative.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| title |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| spellingShingle |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum Castiblanco-Valencia, Mónica M Leptospira Complement Immune evasion Lig proteins Factor H C4BP |
| title_short |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| title_full |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| title_fullStr |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| title_full_unstemmed |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| title_sort |
Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum |
| author |
Castiblanco-Valencia, Mónica M |
| author_facet |
Castiblanco-Valencia, Mónica M Fraga, Tatiana Rodrigues Breda, Leandro Carvalho Dantas Vasconcellos, Sílvio Arruda Figueira, Cláudio Pereira Picardeau, Mathieu Wunder Junior, Elsio Augusto Ko, Albert Icksang Barbosa, Angela Silva Isaac, Lourdes |
| author_role |
author |
| author2 |
Fraga, Tatiana Rodrigues Breda, Leandro Carvalho Dantas Vasconcellos, Sílvio Arruda Figueira, Cláudio Pereira Picardeau, Mathieu Wunder Junior, Elsio Augusto Ko, Albert Icksang Barbosa, Angela Silva Isaac, Lourdes |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Castiblanco-Valencia, Mónica M Fraga, Tatiana Rodrigues Breda, Leandro Carvalho Dantas Vasconcellos, Sílvio Arruda Figueira, Cláudio Pereira Picardeau, Mathieu Wunder Junior, Elsio Augusto Ko, Albert Icksang Barbosa, Angela Silva Isaac, Lourdes |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira Complement Immune evasion Lig proteins Factor H C4BP |
| topic |
Leptospira Complement Immune evasion Lig proteins Factor H C4BP |
| description |
University of São Paulo. Institute of Biomedical Sciences. Department of Immunology. São Paulo, SP, Brasil |
| publishDate |
2016 |
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2016-08-11T16:49:53Z |
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2016-08-11T16:49:53Z |
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2016 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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CASTIBLANCO-VALENCIA, M. M. et al. Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum. Immunology Letters, v. 173, p. 61–68, 2016. |
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https://www.arca.fiocruz.br/handle/icict/15244 |
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0165-2478 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.imlet.2016.03.005 |
| identifier_str_mv |
CASTIBLANCO-VALENCIA, M. M. et al. Acquisition of negative complement regulators by the saprophyte Leptospira biflexa expressing LigA or LigB confers enhanced survival in human serum. Immunology Letters, v. 173, p. 61–68, 2016. 0165-2478 10.1016/j.imlet.2016.03.005 |
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