Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum

Detalhes bibliográficos
Autor(a) principal: Passos, Fabine Correia
Data de Publicação: 2020
Outros Autores: Gois, Marcelo Biondaro, Sousa, Adenilma Duranes, Marinho, Ananda Isis Lima de, Corvo, Laura, Soto, Manoel, Barral Netto, Manoel, Barral, Aldina Maria Prado, Baccan, Gyselle Chrystina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46001
Resumo: Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica, Salvador, BA, Brasil.
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spelling Passos, Fabine CorreiaGois, Marcelo BiondaroSousa, Adenilma DuranesMarinho, Ananda Isis Lima deCorvo, LauraSoto, ManoelBarral Netto, ManoelBarral, Aldina Maria PradoBaccan, Gyselle Chrystina2021-02-09T11:46:02Z2021-02-09T11:46:02Z2020PASSOS, Fabine Correia et al. Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum. Memorias do Instituto Oswaldo Cruz, v. 115, p. e200377, 2020.0074-0276https://www.arca.fiocruz.br/handle/icict/4600110.1590/0074-02760200377engFundação Oswaldo Cruz. Instituto Oswaldo CruzLeishmaniose visceralLeishmania infantumTrato gastrointestinalMicrobiota gastrointestinalVisceral leishmaniasisLeishmania infantumIntestinal tractGut microbiotaInvestigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantuminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica, Salvador, BA, Brasil.Universidade Federal do Recôncavo da Bahia. Centro de Ciências da Saúde. Santo Antônio de Jesus, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica, Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica, Salvador, BA, Brasil.Universidad Autónoma de Madrid. Facultad de Ciencias. Consejo Superior de Investigaciones Científicas. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain.Universidad Autónoma de Madrid. Facultad de Ciencias. Consejo Superior de Investigaciones Científicas. Centro de Biología Molecular Severo Ochoa. Departamento de Biología Molecular. Madrid, Spain.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica, Salvador, BA, Brasil.Visceral leishmaniasis (VL) is a tropical neglected disease with high associated rates of mortality. Several studies have highlighted the importance of the intestinal tract (IT) and gut microbiota (GM) in the host immunological defense. Data in the literature on parasite life cycle and host immune defense against VL are scarce regarding the effects of infection on the IT and GM. OBJECTIVES This study aimed to investigate changes observed in the colon of Leishmania infantum-infected hamsters, including alterations in the enteric nervous system (ENS) and GM (specifically, levels of bifidobacteria and lactobacilli). METHODS Male hamsters were inoculated with L. infantum and euthanised at four or eight months post-infection. Intestines were processed for histological analysis and GM analysis. Quantitative polymerase chain reaction (qPCR) was performed to quantify each group of bacteria: Bifidobacterium spp. (Bf) and Lactobacillus spp (LacB). FINDINGS Infected hamsters showed histoarchitectural loss in the colon wall, with increased thickness in the submucosa and the mucosa layer, as well as greater numbers of intraepithelial lymphocytes. Forms suggestive of amastigotes were seen inside mononuclear cells. L. infantum infection induced changes in ENS, as evidenced by increases in the area of colonic enteric ganglia. Despite the absence of changes in the levels of Bf and LacB during the course of infection, the relative abundance of these bacteria was associated with parasite load and histological alterations. MAIN CONCLUSIONS Our results indicate that L. infantum infection leads to important changes in the colon and suggest that bacteria in the GM play a protective role.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/46001/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALPassos, Fabine Correia Investigating....pdfPassos, Fabine Correia Investigating....pdfapplication/pdf3453164https://www.arca.fiocruz.br/bitstream/icict/46001/2/Passos%2c%20Fabine%20Correia%20Investigating....pdf0f770e27ffaaa80d0e2b3a695efe339fMD52TEXTPassos, Fabine Correia Investigating....pdf.txtPassos, Fabine Correia Investigating....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
title Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
spellingShingle Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
Passos, Fabine Correia
Leishmaniose visceral
Leishmania infantum
Trato gastrointestinal
Microbiota gastrointestinal
Visceral leishmaniasis
Leishmania infantum
Intestinal tract
Gut microbiota
title_short Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
title_full Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
title_fullStr Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
title_full_unstemmed Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
title_sort Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum
author Passos, Fabine Correia
author_facet Passos, Fabine Correia
Gois, Marcelo Biondaro
Sousa, Adenilma Duranes
Marinho, Ananda Isis Lima de
Corvo, Laura
Soto, Manoel
Barral Netto, Manoel
Barral, Aldina Maria Prado
Baccan, Gyselle Chrystina
author_role author
author2 Gois, Marcelo Biondaro
Sousa, Adenilma Duranes
Marinho, Ananda Isis Lima de
Corvo, Laura
Soto, Manoel
Barral Netto, Manoel
Barral, Aldina Maria Prado
Baccan, Gyselle Chrystina
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Passos, Fabine Correia
Gois, Marcelo Biondaro
Sousa, Adenilma Duranes
Marinho, Ananda Isis Lima de
Corvo, Laura
Soto, Manoel
Barral Netto, Manoel
Barral, Aldina Maria Prado
Baccan, Gyselle Chrystina
dc.subject.other.pt_BR.fl_str_mv Leishmaniose visceral
Leishmania infantum
Trato gastrointestinal
Microbiota gastrointestinal
topic Leishmaniose visceral
Leishmania infantum
Trato gastrointestinal
Microbiota gastrointestinal
Visceral leishmaniasis
Leishmania infantum
Intestinal tract
Gut microbiota
dc.subject.en.pt_BR.fl_str_mv Visceral leishmaniasis
Leishmania infantum
Intestinal tract
Gut microbiota
description Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica, Salvador, BA, Brasil.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-02-09T11:46:02Z
dc.date.available.fl_str_mv 2021-02-09T11:46:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PASSOS, Fabine Correia et al. Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum. Memorias do Instituto Oswaldo Cruz, v. 115, p. e200377, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46001
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 10.1590/0074-02760200377
identifier_str_mv PASSOS, Fabine Correia et al. Investigating associations between intestinal alterations and parasite load according to Bifidobacterium spp. and Lactobacillus spp. abundance in the gut microbiota of hamsters infected by Leishmania infantum. Memorias do Instituto Oswaldo Cruz, v. 115, p. e200377, 2020.
0074-0276
10.1590/0074-02760200377
url https://www.arca.fiocruz.br/handle/icict/46001
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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