Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/43303 |
Resumo: | Federal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, Brazil. |
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Dantas, Rebeca C. M.Guimarães, Vanessa Sousa NazaréSouza, Renata O. deValverde, Ludmila de FaroVidal, Manuela T. A.Nogueira, Raphael L. R.Rocha, Leonardo O. S. daAraújo, Giovana T.Santos, Jean N. dosRocha, Clarissa Araújo Gurgel2020-09-10T17:56:48Z2020-09-10T17:56:48Z2020DANTAS, Rebeca C.M. et al. Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma. Applied Immunohistochemistry and Molecular Morphology, 2020.1541-2016https://www.arca.fiocruz.br/handle/icict/43303engLippincott, Williams & WilkinsCarcinoma de células escamosasNeoplasias da bocaProteínaTransição epitelial-mesenquimalProliferação celularSquamous cell carcinomaMouth neoplasmsHedgehog proteinEpithelial-mesenchymal transition,Cell proliferationImmunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinomainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, Brazil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. instituto Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.In oral squamous cell carcinoma (OSCC), involvement and activation of the Hedgehog pathway (HH) may be related to epithelial-mesenchymal transition and cell proliferation. The present study aimed to evaluate epithelial-mesenchymal transition and proliferative potential in OSCC cases demonstrating activation of the HH pathway. Twenty-three GLi-1-positive OSCC cases were submitted to immunohistochemical detection of Snail, Slug, N-cadherin, E-cadherin, β-catenin, and MCM3 proteins. Clinicalpathologic immunoexpression data were obtained from the invasion front and tumor islets, and then compared. At the invasion front, OSCC cases presented positive Snail, Slug, and MCM3 expression in the nuclei of tumor cells. Loss of membrane and cytoplasmic expression of E-cadherin and β-catenin was also observed. Positive N-cadherin expression was observed in 31.78% of the cases. GLi-1 immunoexpression was associated with loss of membrane E-cadherin (P<0.001), membrane β-catenin (P<0.001), and cytoplasmic β-catenin (P=0.02) expression. In the tumor islets, we observed nuclear expression of GLi-1, Snail, Slug, and MCM3. E-cadherin and β-catenin showed positivity in tumor cell membranes. Statistically significant positive correlations between GLi-1 and Snail (P=0.05), E-cadherin (P=0.01), and cytoplasmic β-catenin (P=0.04) were found. GLi-1 was associated with clinical staging, while membrane β-catenin expression was related to the presence of metastasis in lymph nodes and to clinical staging. The HH pathway may be involved in regulating the expression of the mesenchymal phenotype. The loss of membrane E-cadherin and β-catenin expression was observed at the tumor front region, whereas cell adhesion protein expression was detected in tumor islets regardless of MCM3.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43303/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDantas R.C.M Immunodetection....pdfDantas R.C.M Immunodetection....pdfapplication/pdf689672https://www.arca.fiocruz.br/bitstream/icict/43303/2/Dantas%20R.C.M%20Immunodetection....pdf9ef0740b04a14299b852d9da52e7c8e6MD52TEXTDantas R.C.M Immunodetection....pdf.txtDantas R.C.M Immunodetection....pdf.txtExtracted texttext/plain39146https://www.arca.fiocruz.br/bitstream/icict/43303/3/Dantas%20R.C.M%20Immunodetection....pdf.txt057902fd6e0a94aa73a06a57e60dc73cMD53icict/433032023-03-15 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| dc.title.pt_BR.fl_str_mv |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| title |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| spellingShingle |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma Dantas, Rebeca C. M. Carcinoma de células escamosas Neoplasias da boca Proteína Transição epitelial-mesenquimal Proliferação celular Squamous cell carcinoma Mouth neoplasms Hedgehog protein Epithelial-mesenchymal transition, Cell proliferation |
| title_short |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| title_full |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| title_fullStr |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| title_full_unstemmed |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| title_sort |
Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma |
| author |
Dantas, Rebeca C. M. |
| author_facet |
Dantas, Rebeca C. M. Guimarães, Vanessa Sousa Nazaré Souza, Renata O. de Valverde, Ludmila de Faro Vidal, Manuela T. A. Nogueira, Raphael L. R. Rocha, Leonardo O. S. da Araújo, Giovana T. Santos, Jean N. dos Rocha, Clarissa Araújo Gurgel |
| author_role |
author |
| author2 |
Guimarães, Vanessa Sousa Nazaré Souza, Renata O. de Valverde, Ludmila de Faro Vidal, Manuela T. A. Nogueira, Raphael L. R. Rocha, Leonardo O. S. da Araújo, Giovana T. Santos, Jean N. dos Rocha, Clarissa Araújo Gurgel |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Dantas, Rebeca C. M. Guimarães, Vanessa Sousa Nazaré Souza, Renata O. de Valverde, Ludmila de Faro Vidal, Manuela T. A. Nogueira, Raphael L. R. Rocha, Leonardo O. S. da Araújo, Giovana T. Santos, Jean N. dos Rocha, Clarissa Araújo Gurgel |
| dc.subject.other.pt_BR.fl_str_mv |
Carcinoma de células escamosas Neoplasias da boca Proteína Transição epitelial-mesenquimal Proliferação celular |
| topic |
Carcinoma de células escamosas Neoplasias da boca Proteína Transição epitelial-mesenquimal Proliferação celular Squamous cell carcinoma Mouth neoplasms Hedgehog protein Epithelial-mesenchymal transition, Cell proliferation |
| dc.subject.en.pt_BR.fl_str_mv |
Squamous cell carcinoma Mouth neoplasms Hedgehog protein Epithelial-mesenchymal transition, Cell proliferation |
| description |
Federal University of Bahia. School of Dentistry. Laboratory of Oral Surgical Pathology. Salvador, BA, Brazil. |
| publishDate |
2020 |
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2020-09-10T17:56:48Z |
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2020-09-10T17:56:48Z |
| dc.date.issued.fl_str_mv |
2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
DANTAS, Rebeca C.M. et al. Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma. Applied Immunohistochemistry and Molecular Morphology, 2020. |
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https://www.arca.fiocruz.br/handle/icict/43303 |
| dc.identifier.issn.pt_BR.fl_str_mv |
1541-2016 |
| identifier_str_mv |
DANTAS, Rebeca C.M. et al. Immunodetection of Epithelial-Mesenchymal Transition and Tumor Proliferation Markers in GLi-1-positive Oral Squamous Cell Carcinoma. Applied Immunohistochemistry and Molecular Morphology, 2020. 1541-2016 |
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https://www.arca.fiocruz.br/handle/icict/43303 |
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Lippincott, Williams & Wilkins |
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Lippincott, Williams & Wilkins |
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