Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/46082 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
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Horinouchi, Cintia D. S.Barisón, María JuliaRobert, Anny WaloskiKuligovski, CriscieleAguiar, Alessandra Melo deDallagiovanna, Bruno2021-02-15T16:06:37Z2021-02-15T16:06:37Z2020HORINOUCHI, Cintia D. S. et al. Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells. World Journal Stem Cells, v.12, n. 12, p. 1640-1651, 2020.1948-0210https://www.arca.fiocruz.br/handle/icict/4608210.4252/wjsc.v12.i12.1640porBaishideng Publishing GroupCélulas-Tronco MesenquimaisMesenchymal Stem CellsAdipogenesisOsteogenesisAge FactorsCélulas Madre MesenquimatosasAdipogénesisOsteogénesisFactores de EdadCellules souches mésenchymateusesAdipogenèseOstéogenèseFacteurs âgesAdipogêneseOsteogêneseDiferenciação CelularFatores EtáriosInfluence of donor age on the differentiation and division capacity of human adipose-derived stem cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Human adipose-derived stromal/stem cells (hASCs) are one of the most useful types of mesenchymal stromal/stem cells, which are adult multipotent cells with great therapeutic potential for the treatment of several diseases. However, for successful clinical application, it is critical that high-quality cells can be obtained. Diverse factors seem to be able to influence cell quality and performance, especially factors related to donors’ intrinsic characteristics, such as age. Nevertheless, there is no consensus regarding this characteristic, and there is conflicting information in the literature. To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors. hASCs were harvested from liposuctioned adipose tissue obtained from female donors (aged 20-70 years). Cells were distributed into two groups according to age range: old hASCs (oASCs, ≥ 55 years, n = 9) and young hASCs (yASCs, ≤ 35 years, n = 9). For each group, immunophenotypic characterization was performed by flow cytometry. Population doubling time was assessed over seven days. For adipogenic potential evaluation, lipid deposits were assessed after 7 d, 14 d and 21 d of adipogenic induction. Osteogenic potential was verified by analyzing cell mineralization after 14 d, 21 d and 28 d of osteogenic induction. mRNA expression of PPARγ2, CEBPA and Runx hASCs were successfully obtained, cultured, and grouped according to their age: yASCs (26.33 ± 4.66 years old) and oASCs (64.78 ± 4.58 years old). After maintenance of the cells in culture, there were no differences in morphology between cells from the young and old donors. Additionally, both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells. The average doubling time indicated that yASCs (4.09 ± 0.94 d) did not significantly differ from oASCs (4.19 ± 1.29 d). Concerning differentiation potential, after adipogenic and osteogenic induction, yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells. However, no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis. Additionally, the mRNA expression of PPARγ2, CEBPA and Runx2 were similar in yASCs and oASCs. Our findings suggest that age does not seem to significantly affect the cell Division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/46082/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALWJSC-12-1640ok.pdfWJSC-12-1640ok.pdfapplication/pdf8575166https://www.arca.fiocruz.br/bitstream/icict/46082/2/WJSC-12-1640ok.pdf98afb5b7382d34df224dd73d18c1f56bMD52TEXTWJSC-12-1640ok.pdf.txtWJSC-12-1640ok.pdf.txtExtracted texttext/plain45210https://www.arca.fiocruz.br/bitstream/icict/46082/3/WJSC-12-1640ok.pdf.txt8caa42816cd9e0e0434feca754c41893MD53icict/460822021-02-22 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dc.title.pt_BR.fl_str_mv |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
title |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
spellingShingle |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells Horinouchi, Cintia D. S. Células-Tronco Mesenquimais Mesenchymal Stem Cells Adipogenesis Osteogenesis Age Factors Células Madre Mesenquimatosas Adipogénesis Osteogénesis Factores de Edad Cellules souches mésenchymateuses Adipogenèse Ostéogenèse Facteurs âges Adipogênese Osteogênese Diferenciação Celular Fatores Etários |
title_short |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
title_full |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
title_fullStr |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
title_full_unstemmed |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
title_sort |
Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells |
author |
Horinouchi, Cintia D. S. |
author_facet |
Horinouchi, Cintia D. S. Barisón, María Julia Robert, Anny Waloski Kuligovski, Crisciele Aguiar, Alessandra Melo de Dallagiovanna, Bruno |
author_role |
author |
author2 |
Barisón, María Julia Robert, Anny Waloski Kuligovski, Crisciele Aguiar, Alessandra Melo de Dallagiovanna, Bruno |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Horinouchi, Cintia D. S. Barisón, María Julia Robert, Anny Waloski Kuligovski, Crisciele Aguiar, Alessandra Melo de Dallagiovanna, Bruno |
dc.subject.other.pt_BR.fl_str_mv |
Células-Tronco Mesenquimais |
topic |
Células-Tronco Mesenquimais Mesenchymal Stem Cells Adipogenesis Osteogenesis Age Factors Células Madre Mesenquimatosas Adipogénesis Osteogénesis Factores de Edad Cellules souches mésenchymateuses Adipogenèse Ostéogenèse Facteurs âges Adipogênese Osteogênese Diferenciação Celular Fatores Etários |
dc.subject.en.pt_BR.fl_str_mv |
Mesenchymal Stem Cells Adipogenesis Osteogenesis Age Factors |
dc.subject.es.pt_BR.fl_str_mv |
Células Madre Mesenquimatosas Adipogénesis Osteogénesis Factores de Edad |
dc.subject.fr.pt_BR.fl_str_mv |
Cellules souches mésenchymateuses Adipogenèse Ostéogenèse Facteurs âges |
dc.subject.decs.pt_BR.fl_str_mv |
Adipogênese Osteogênese Diferenciação Celular Fatores Etários |
description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2021-02-15T16:06:37Z |
dc.date.available.fl_str_mv |
2021-02-15T16:06:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
HORINOUCHI, Cintia D. S. et al. Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells. World Journal Stem Cells, v.12, n. 12, p. 1640-1651, 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/46082 |
dc.identifier.issn.pt_BR.fl_str_mv |
1948-0210 |
dc.identifier.doi.none.fl_str_mv |
10.4252/wjsc.v12.i12.1640 |
identifier_str_mv |
HORINOUCHI, Cintia D. S. et al. Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells. World Journal Stem Cells, v.12, n. 12, p. 1640-1651, 2020. 1948-0210 10.4252/wjsc.v12.i12.1640 |
url |
https://www.arca.fiocruz.br/handle/icict/46082 |
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por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
Baishideng Publishing Group |
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Baishideng Publishing Group |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
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Repositório Institucional da FIOCRUZ (ARCA) |
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