Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25070 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), by Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Financiadora de Estudos e Projetos (FINEP). |
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Daltro, Pamela SantanaBarreto, Breno CardimSilva, P GChenaud Neto, PauloSousa Filho, P H FSantana Neta, DCarvalho, Gisele BatistaSilva, D NParedes, Bruno DiazAlcantara, Adriano Costa deFreitas, Luiz Antonio Rodrigues deCouto, Ricardo DavidSantos, Ricardo Ribeiro dosSouza, Bruno Solano de FreitasSoares, Milena Botelho PereiraMacambira, Simone Garcia2018-03-06T13:39:29Z2018-03-06T13:39:29Z2017DALTRO, P. S. et al. Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model. Cytotherapy, v. 19, p. 1176–1188, 2017.1465-3249https://www.arca.fiocruz.br/handle/icict/2507010.1016/j.jcyt.2017.07.002Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), by Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Financiadora de Estudos e Projetos (FINEP).Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Federal University of Bahia. Multicentric Program in Biochemistry and Molecular Biology. Salvador, BA, BrazilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Federal University of Bahia. Faculty of Biology. Salvador, BA, BrazilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Faculty of Pharmacy. Salvador, BA, BrazilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / National Institute of Science andTechnology for Regenerative Medicine. Rio de Janeiro, RJ, BrazilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science andTechnology for Regenerative Medicine. Rio de Janeiro, RJ, BrazilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science andTechnology for Regenerative Medicine. Rio de Janeiro, RJ, BrazilHospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science andTechnology for Regenerative Medicine. Rio de Janeiro, RJ, Brazil / Federal University of Bahia. Institute of Health Sciences. Department of Biochemistry and Biophysics. Salvador, BA, BrazilObesity is associated with numerous cardiac complications, including arrhythmias, cardiac fibrosis, remodeling and heart failure. Here we evaluated the therapeutic potential of mesenchymal stromal cells (MSCs) and their conditioned medium (CM) to treat cardiac complications in a mouse model of high-fat diet (HFD)-induced obesity.Methods. After obesity induction and HFD withdrawal, obese mice were treated with MSCs, CM or vehicle. Cardiac function was assessed using electrocardiography, echocardiography and treadmill test. Body weight and biochemical parameters were evaluated. Cardiac tissue was used for real time (RT)-polymerase chain reaction (PCR) and histopathologic analysis. Results/ Discussion. Characterization of CM by protein array showed the presence of different cytokines and growth factors, including chemokines, osteopontin, cystatin C, Serpin E1 and Gas 6. HFD-fed mice presented cardiac arrhythmias, altered cardiac gene expression and fibrosis reflected in physical exercise incapacity associated with obesity and diabetes. Administration of MSCs or CM improved arrhythmias and exercise capacity. This functional improvement correlated with normalization of GATA4 gene expression in the hearts of MSC- or CM-treated mice. The gene expression of connexin 43, troponin I, adiponectin, transforming growth factor (TGF) β, peroxisome proliferator activated receptor gamma (PPARγ), insulin-like growth factor 1 (IGF-1), matrix metalloproteinase-9 (MMP9) and tissue inhibitor of metalloproteinases 1 (TIMP1) were significantly reduced in MSCs, but not in CM-treated mice. Moreover, MSC or CM administration reduced the intensity of cardiac fibrosis. Conclusion. Our results suggest that MSCs and CM have a recovery effect on cardiac disturbances due to obesity and corroborate to the paracrine action of MSCs in heart disease models.engElsevierDisfunção cardíacaTerapia celularCélulas estomatais mesenquimatosasObesidadeCardiac dysfunctionCell therapyMesenchymal stromal cellsObesityTherapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25070/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDaltro PS Therapy with mesenchymal stromal....pdfDaltro PS Therapy with mesenchymal 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| dc.title.pt_BR.fl_str_mv |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| title |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| spellingShingle |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model Daltro, Pamela Santana Disfunção cardíaca Terapia celular Células estomatais mesenquimatosas Obesidade Cardiac dysfunction Cell therapy Mesenchymal stromal cells Obesity |
| title_short |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| title_full |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| title_fullStr |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| title_full_unstemmed |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| title_sort |
Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model |
| author |
Daltro, Pamela Santana |
| author_facet |
Daltro, Pamela Santana Barreto, Breno Cardim Silva, P G Chenaud Neto, Paulo Sousa Filho, P H F Santana Neta, D Carvalho, Gisele Batista Silva, D N Paredes, Bruno Diaz Alcantara, Adriano Costa de Freitas, Luiz Antonio Rodrigues de Couto, Ricardo David Santos, Ricardo Ribeiro dos Souza, Bruno Solano de Freitas Soares, Milena Botelho Pereira Macambira, Simone Garcia |
| author_role |
author |
| author2 |
Barreto, Breno Cardim Silva, P G Chenaud Neto, Paulo Sousa Filho, P H F Santana Neta, D Carvalho, Gisele Batista Silva, D N Paredes, Bruno Diaz Alcantara, Adriano Costa de Freitas, Luiz Antonio Rodrigues de Couto, Ricardo David Santos, Ricardo Ribeiro dos Souza, Bruno Solano de Freitas Soares, Milena Botelho Pereira Macambira, Simone Garcia |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Daltro, Pamela Santana Barreto, Breno Cardim Silva, P G Chenaud Neto, Paulo Sousa Filho, P H F Santana Neta, D Carvalho, Gisele Batista Silva, D N Paredes, Bruno Diaz Alcantara, Adriano Costa de Freitas, Luiz Antonio Rodrigues de Couto, Ricardo David Santos, Ricardo Ribeiro dos Souza, Bruno Solano de Freitas Soares, Milena Botelho Pereira Macambira, Simone Garcia |
| dc.subject.other.pt_BR.fl_str_mv |
Disfunção cardíaca Terapia celular Células estomatais mesenquimatosas Obesidade |
| topic |
Disfunção cardíaca Terapia celular Células estomatais mesenquimatosas Obesidade Cardiac dysfunction Cell therapy Mesenchymal stromal cells Obesity |
| dc.subject.en.pt_BR.fl_str_mv |
Cardiac dysfunction Cell therapy Mesenchymal stromal cells Obesity |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), by Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB) and Financiadora de Estudos e Projetos (FINEP). |
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2017 |
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2017 |
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2018-03-06T13:39:29Z |
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2018-03-06T13:39:29Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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DALTRO, P. S. et al. Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model. Cytotherapy, v. 19, p. 1176–1188, 2017. |
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https://www.arca.fiocruz.br/handle/icict/25070 |
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1465-3249 |
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10.1016/j.jcyt.2017.07.002 |
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DALTRO, P. S. et al. Therapy with mesenchymal stromal cells or conditioned medium reverse cardiac alterations in a high-fat diet-induced obesity model. Cytotherapy, v. 19, p. 1176–1188, 2017. 1465-3249 10.1016/j.jcyt.2017.07.002 |
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Elsevier |
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