Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear

Detalhes bibliográficos
Autor(a) principal: Carregaro, Vanessa
Data de Publicação: 2013
Outros Autores: Costa, Diego Luis, Brodskyn, Claudia Ida, Barral, Aldina Maria Prado, Barral Netto, Manoel, Cunha, Fernando Queiroz, Silva, João Santana da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7263
Resumo: University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil
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spelling Carregaro, VanessaCosta, Diego LuisBrodskyn, Claudia IdaBarral, Aldina Maria PradoBarral Netto, ManoelCunha, Fernando QueirozSilva, João Santana da2013-11-26T16:02:48Z2013-11-26T16:02:48Z2013CARREGARO, V. et al. Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear. BMC Microbiology (Online), v.13, p.102-112, 2013.1471-2199https://www.arca.fiocruz.br/handle/icict/726310.1186/1471-2180-13-102engBioMed CentralDual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice earinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, BrasilUniversity of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil / Institute Immunological Research. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil / Institute Immunological Research. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. School of Medicine. Salvador, BA, Brasil / Institute Immunological Research. Salvador, BA, BrasilUniversity of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil / University of São Paulo. School of Medicine. Department of Pharmacology. Ribeirão Preto, SP, Brasil.University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, BrasilLeishmania parasites are transmitted to their vertebrate hosts by infected Phlebotomine sand flies during the blood meal of the flies. Sand fly saliva is known to enhance Leishmania spp. infection, while preexposure to saliva protects mice against parasitic infections. In this study, we investigated the initial inflammatory leucocyte composition induced by one or three inocula of salivary gland extract (SGE) from Lutzomyia longipalpis in the presence or absence of Leishmania braziliensis. Results: We demonstrated that inoculating SGE once (SGE-1X) or three times (SGE-3X), which represented a coinoculation or a pre-exposure to saliva, respectively, resulted in different cellular infiltrate profiles. Whereas SGE-1X led to the recruitment of all leucocytes subtypes including CD4+ T cells, CD4+CD25+ T cells, dendritic cells, macrophages and neutrophils, the immune cell profile in the SGE-3X group differed dramatically, as CD4+ T cells, CD4+CD25+ T cells, dendritic cells, macrophages and neutrophils were decreased and CD8+ T cells were increased. The SGE-1X group did not show differences in the ear lesion size; however, the SGE-1X group harbored a higher number of parasites. On the other hand, the SGE-3X group demonstrated a protective effect against parasitic disease, as the parasite burden was lower even in the earlier stages of the infection, a period in which the SGE-1X group presented with larger and more severe lesions. These effects were also reflected in the cytokine profiles of both groups. Whereas the SGE-1X group presented with a substantial increase in IL-10 production, the SGE-3X group showed an increase in IFN-γ production in the draining lymph nodes. Analysis of the inflammatory cell populations present within the ear lesions, the SGE-1X group showed an increase in CD4+FOXP3+ cells, whereas the CD4+FOXP3+ population was reduced in the SGE-3X group. Moreover, CD4+ T cells and CD8+ T cells producing IFN-γ were highly detected in the ears of the SGE-3X mice prior to infection. In addition, upon treatment of SGE-3X mice with anti-IFN-γ monoclonal antibody, we observed a decrease in the protective effect of SGE-3X against L. braziliensis infection. Conclusions: These results indicate that different inocula of Lutzomyia longipalpis salivary gland extract can markedly modify the cellular immune response, which is reflected in the pattern of susceptibility or resistance to Leishmania braziliensis infection.Phlebotomines saliva,Lutzomyia longipalpis salivaLeishmania braziliensisInflammatory leucocytesCytokinesImmunoregulationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALCarregaro V Dual effect....pdfCarregaro V Dual effect....pdfapplication/pdf842320https://www.arca.fiocruz.br/bitstream/icict/7263/1/Carregaro%20V%20Dual%20effect....pdf6965b9264443601e5fa269bef50cd910MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7263/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTCarregaro V Dual effect....pdf.txtCarregaro V Dual effect....pdf.txtExtracted texttext/plain51809https://www.arca.fiocruz.br/bitstream/icict/7263/5/Carregaro%20V%20Dual%20effect....pdf.txt58eb7b6c38f3887df4a3f63df5f7b62cMD55THUMBNAILCarregaro V Dual effect....pdf.jpgCarregaro V Dual effect....pdf.jpgGenerated Thumbnailimage/jpeg2313https://www.arca.fiocruz.br/bitstream/icict/7263/4/Carregaro%20V%20Dual%20effect....pdf.jpg25c13f5efe615e7dd16e168c1fc6544aMD54icict/72632023-03-15 14:33:11.432oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:11Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
spellingShingle Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
Carregaro, Vanessa
Phlebotomines saliva,
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
title_short Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_full Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_fullStr Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_full_unstemmed Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
title_sort Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear
author Carregaro, Vanessa
author_facet Carregaro, Vanessa
Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Cunha, Fernando Queiroz
Silva, João Santana da
author_role author
author2 Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Cunha, Fernando Queiroz
Silva, João Santana da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carregaro, Vanessa
Costa, Diego Luis
Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Barral Netto, Manoel
Cunha, Fernando Queiroz
Silva, João Santana da
dc.subject.en.pt_BR.fl_str_mv Phlebotomines saliva,
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
topic Phlebotomines saliva,
Lutzomyia longipalpis saliva
Leishmania braziliensis
Inflammatory leucocytes
Cytokines
Immunoregulation
description University of São Paulo. School of Medicine. Department of Biochemistry and Immunology. Ribeirão Preto, SP, Brasil
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-11-26T16:02:48Z
dc.date.available.fl_str_mv 2013-11-26T16:02:48Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARREGARO, V. et al. Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear. BMC Microbiology (Online), v.13, p.102-112, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7263
dc.identifier.issn.none.fl_str_mv 1471-2199
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2180-13-102
identifier_str_mv CARREGARO, V. et al. Dual effect of Lutzomyia longipalpis saliva on Leishmania braziliensis infection is mediated by distinct saliva-induced cellular recruitment into BALB/c mice ear. BMC Microbiology (Online), v.13, p.102-112, 2013.
1471-2199
10.1186/1471-2180-13-102
url https://www.arca.fiocruz.br/handle/icict/7263
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
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instname:Fundação Oswaldo Cruz (FIOCRUZ)
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