Infection with Leishmania major Induces a Cellular Stress Response in Macrophages

Detalhes bibliográficos
Autor(a) principal: Filardy, Alessandra A
Data de Publicação: 2014
Outros Autores: Silva, Ana Carolina Costa da, Koeller, Carolina M, Pinto, Kamila Guimarães, Gomes, Flávia L. Ribeiro, Lopes, Marcela F, Heise, Norton, Lima, Célio G. Freire de, Nunes, Marise P, Reis, George A. dos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10524
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
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spelling Filardy, Alessandra ASilva, Ana Carolina Costa daKoeller, Carolina MPinto, Kamila GuimarãesGomes, Flávia L. RibeiroLopes, Marcela FHeise, NortonLima, Célio G. Freire deNunes, Marise PReis, George A. dos2015-05-27T13:39:43Z2015-05-27T13:39:43Z2014FILARDY, Alessandra A. et al. Infection with Leishmania major Induces a Cellular Stress Response in Macrophages. Plos One, v.9, n.1, 9p, jan. 2014.https://www.arca.fiocruz.br/handle/icict/1052410.1371/journal.pone.0085715engPlos OneLeishmanioseLeishmania majorMacrophagesCellular StressInfectionInfection with Leishmania major Induces a Cellular Stress Response in Macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.National Institute of Health. Laboratory of Parasitic Diseases. Bethesda, MA, USA.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.We investigated early cellular responses induced by infection with Leishmania major in macrophages from resistant C57/BL6 mice. Infection increased production of reactive oxygen species by resident, but not inflammatory peritoneal macrophages. In addition, infection increased activation of stress-activated protein kinases/c-Jun N-terminal kinases (SAPK/JNK) in resident, but not in inflammatory peritoneal macrophages. Infection also increased expression of membrane and soluble FasL, but infected macrophages remained viable after 48 h. Infection increased secretion of cytokines/chemokines TNF-α, IL-6, TIMP-1, IL-1RA, G-CSF, TREM, KC, MIP-1α, MIP-1β, MCP-1, and MIP-2 in resident macrophages. Addition of antioxidants deferoxamine and N-acetylcysteine reduced ROS generation and JNK activation. Addition of antioxidants or JNK inhibitor SP600125 reduced secretion of KC. Furthermore, treatment with antioxidants or JNK inhibitor also reduced intracellular parasite replication. These results indicated that infection triggers a rapid cellular stress response in resident macrophages which induces proinflammatory signals, but is also involved in parasite survival and replication in host macrophages.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10524/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmarise_nunesetal_IOC_2014.pdfapplication/pdf991260https://www.arca.fiocruz.br/bitstream/icict/10524/2/marise_nunesetal_IOC_2014.pdf9a761dfc21ca56fafd41c0c53b2b8169MD52TEXTmarise_nunesetal_IOC_2014.pdf.txtmarise_nunesetal_IOC_2014.pdf.txtExtracted texttext/plain44682https://www.arca.fiocruz.br/bitstream/icict/10524/3/marise_nunesetal_IOC_2014.pdf.txt80a47a029d7f364f4fd81f59f2373fd8MD53icict/105242022-06-24 13:03:02.895oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:03:02Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
title Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
spellingShingle Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
Filardy, Alessandra A
Leishmaniose
Leishmania major
Macrophages
Cellular Stress
Infection
title_short Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
title_full Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
title_fullStr Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
title_full_unstemmed Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
title_sort Infection with Leishmania major Induces a Cellular Stress Response in Macrophages
author Filardy, Alessandra A
author_facet Filardy, Alessandra A
Silva, Ana Carolina Costa da
Koeller, Carolina M
Pinto, Kamila Guimarães
Gomes, Flávia L. Ribeiro
Lopes, Marcela F
Heise, Norton
Lima, Célio G. Freire de
Nunes, Marise P
Reis, George A. dos
author_role author
author2 Silva, Ana Carolina Costa da
Koeller, Carolina M
Pinto, Kamila Guimarães
Gomes, Flávia L. Ribeiro
Lopes, Marcela F
Heise, Norton
Lima, Célio G. Freire de
Nunes, Marise P
Reis, George A. dos
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Filardy, Alessandra A
Silva, Ana Carolina Costa da
Koeller, Carolina M
Pinto, Kamila Guimarães
Gomes, Flávia L. Ribeiro
Lopes, Marcela F
Heise, Norton
Lima, Célio G. Freire de
Nunes, Marise P
Reis, George A. dos
dc.subject.other.pt_BR.fl_str_mv Leishmaniose
topic Leishmaniose
Leishmania major
Macrophages
Cellular Stress
Infection
dc.subject.en.pt_BR.fl_str_mv Leishmania major
Macrophages
Cellular Stress
Infection
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-27T13:39:43Z
dc.date.available.fl_str_mv 2015-05-27T13:39:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FILARDY, Alessandra A. et al. Infection with Leishmania major Induces a Cellular Stress Response in Macrophages. Plos One, v.9, n.1, 9p, jan. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10524
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0085715
identifier_str_mv FILARDY, Alessandra A. et al. Infection with Leishmania major Induces a Cellular Stress Response in Macrophages. Plos One, v.9, n.1, 9p, jan. 2014.
10.1371/journal.pone.0085715
url https://www.arca.fiocruz.br/handle/icict/10524
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Plos One
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