Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania

Detalhes bibliográficos
Autor(a) principal: Gomes, Ivana Nunes
Data de Publicação: 2003
Outros Autores: Calabrich, Aknar Freire de Carvalho, Tavares, Rafael da Silva, Wietzerbin, Jeanne, Freitas, Luiz Antonio Rodrigues de, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1016/S1286-4579(03)00025-X
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7751
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
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spelling Gomes, Ivana NunesCalabrich, Aknar Freire de CarvalhoTavares, Rafael da SilvaWietzerbin, JeanneFreitas, Luiz Antonio Rodrigues deVeras, Patrícia Sampaio Tavares2014-06-02T17:55:52Z2014-06-02T17:55:52Z2003GOMES, I. N. et al. Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania. Microbes and Infection, v. 5, n. 4, p. 251-260, 2003.1286-4579https://www.arca.fiocruz.br/handle/icict/775110.1016/S1286-4579(03)00025-XengElsevierDifferential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmaniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina da Universidade Federal da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil.Unité Inserm U365. Institut Curie. Paris, France.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina da Universidade Federal da Bahia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.While CBA/J mice fail to be permissive to Leishmania amazonensis-driven pathogenic processes, they heal easily following Leishmania major infection. The early-phase events are crucial to the outcome of Leishmania infection and it is known that macrophages (Mφ) are important in infection control. In the present study we investigated the role of Mφ in driving CBA/J susceptibility to L. amazonensis. We performed kinetic studies and compared the capacity of L. amazonensis and L. major to infect Mφ. There was no difference in percentages of infection or parasite burden for 6 h between the two groups. In contrast, after 12 h we observed that infection was about twice as high in L. amazonensis- than in L. major-infectedMφ. In addition, rIFN-c added to the cultures induced nitric oxide (NO) production, and did not modify L. amazonensis infection, although the percentage of L. major infection was significantly reduced. This reduction in L. major infection is a TNF-a dependent mechanism as L. major-infected Mφ expressed twice as much TNF-a mRNA as L. amazonensis-infected cells, and anti-TNF-a reversed the IFN-c effect. Moreover, rTNF-a plus IFN-c were able to significantly reduce the percentage of L. amazonensisinfected cells but not to the same extent as in L. major infection. Despite having higher NO production than IFN-c-treated cells,AMGaddition to IFN-c-plus TNF-a-treated cells only partially reversed the inhibition in L. major, but not in L. amazonensis infection. Thus, in this study, we demonstrated that L. amazonensis both inactivated and resisted innate and IFN-c-induced Mφ killing mechanisms, indicating that the nature of the parasite and its interaction with Mφ could determine immune response polarization.MacrophagesCBA/J miceL. amazonensisLeishmaniose/imunologiaMacrófagos/imunologiaMacrófagos/parasitologiaCélulas CultivadasAnimaisFemininoPeróxido de Hidrogênio/metabolismoInflamação/imunologiaInterferon gama/metabolismoInterleucina-10/farmacologiaLeishmania/classificaçãoLeishmania/efeitos de drogasLeishmania/patogenicidadeLeishmaniose/parasitologiaLeishmaniose/patologiaLeishmaniose Cutânea/patologiaMasculinoCamundongosCamundongos Endogâmicos CBAEspecificidade da EspécieFator de Crescimento Transformador beta/farmacologiaFator de Necrose Tumoral alfa/fisiologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALGomes IN Differential properties....pdfGomes IN Differential properties....pdfapplication/pdf354372https://www.arca.fiocruz.br/bitstream/icict/7751/1/Gomes%20IN%20Differential%20properties....pdf68ab69b5cc5e7adefa3c142fb6a161feMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7751/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTGomes IN Differential properties....pdf.txtGomes IN Differential properties....pdf.txtExtracted texttext/plain49911https://www.arca.fiocruz.br/bitstream/icict/7751/5/Gomes%20IN%20Differential%20properties....pdf.txtaba7ae52a12a49941b40c81785c33a2aMD55THUMBNAILGomes IN Differential properties....pdf.jpgGomes IN Differential properties....pdf.jpgGenerated Thumbnailimage/jpeg2374https://www.arca.fiocruz.br/bitstream/icict/7751/4/Gomes%20IN%20Differential%20properties....pdf.jpgf8868a3219452943a83b9a41c409568cMD54icict/77512023-03-15 14:33:17.639oai:www.arca.fiocruz.br:icict/7751TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
title Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
spellingShingle Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
Gomes, Ivana Nunes
Macrophages
CBA/J mice
L. amazonensis
Leishmaniose/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Células Cultivadas
Animais
Feminino
Peróxido de Hidrogênio/metabolismo
Inflamação/imunologia
Interferon gama/metabolismo
Interleucina-10/farmacologia
Leishmania/classificação
Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose/parasitologia
Leishmaniose/patologia
Leishmaniose Cutânea/patologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Especificidade da Espécie
Fator de Crescimento Transformador beta/farmacologia
Fator de Necrose Tumoral alfa/fisiologia
title_short Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
title_full Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
title_fullStr Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
title_full_unstemmed Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
title_sort Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania
author Gomes, Ivana Nunes
author_facet Gomes, Ivana Nunes
Calabrich, Aknar Freire de Carvalho
Tavares, Rafael da Silva
Wietzerbin, Jeanne
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
author_role author
author2 Calabrich, Aknar Freire de Carvalho
Tavares, Rafael da Silva
Wietzerbin, Jeanne
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Ivana Nunes
Calabrich, Aknar Freire de Carvalho
Tavares, Rafael da Silva
Wietzerbin, Jeanne
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
dc.subject.en.pt_BR.fl_str_mv Macrophages
CBA/J mice
L. amazonensis
topic Macrophages
CBA/J mice
L. amazonensis
Leishmaniose/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Células Cultivadas
Animais
Feminino
Peróxido de Hidrogênio/metabolismo
Inflamação/imunologia
Interferon gama/metabolismo
Interleucina-10/farmacologia
Leishmania/classificação
Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose/parasitologia
Leishmaniose/patologia
Leishmaniose Cutânea/patologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Especificidade da Espécie
Fator de Crescimento Transformador beta/farmacologia
Fator de Necrose Tumoral alfa/fisiologia
dc.subject.decs.pt_BR.fl_str_mv Leishmaniose/imunologia
Macrófagos/imunologia
Macrófagos/parasitologia
Células Cultivadas
Animais
Feminino
Peróxido de Hidrogênio/metabolismo
Inflamação/imunologia
Interferon gama/metabolismo
Interleucina-10/farmacologia
Leishmania/classificação
Leishmania/efeitos de drogas
Leishmania/patogenicidade
Leishmaniose/parasitologia
Leishmaniose/patologia
Leishmaniose Cutânea/patologia
Masculino
Camundongos
Camundongos Endogâmicos CBA
Especificidade da Espécie
Fator de Crescimento Transformador beta/farmacologia
Fator de Necrose Tumoral alfa/fisiologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2014-06-02T17:55:52Z
dc.date.available.fl_str_mv 2014-06-02T17:55:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, I. N. et al. Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania. Microbes and Infection, v. 5, n. 4, p. 251-260, 2003.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7751
dc.identifier.issn.none.fl_str_mv 1286-4579
dc.identifier.doi.none.fl_str_mv 10.1016/S1286-4579(03)00025-X
identifier_str_mv GOMES, I. N. et al. Differential properties of CBA/J mononuclear phagocytes recovered from an inflammatory site and probed with two different species of Leishmania. Microbes and Infection, v. 5, n. 4, p. 251-260, 2003.
1286-4579
10.1016/S1286-4579(03)00025-X
url https://www.arca.fiocruz.br/handle/icict/7751
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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