Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load

Detalhes bibliográficos
Autor(a) principal: Aoki, Mateus Nóbrega
Data de Publicação: 2021
Outros Autores: Coelho, Bruna de Oliveira, Góes, Luiz Gustavo Bentim, Minoprio, Paola, Durigon, Edison Luiz, Morello, Luis Gustavo, Marchini, Fabrício Klerynton, Riediger, Irina Natassja, Debur, Maria do Carmo, Nakaya, Helder I., Blanes, Lucas
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47062
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.
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spelling Aoki, Mateus NóbregaCoelho, Bruna de OliveiraGóes, Luiz Gustavo BentimMinoprio, PaolaDurigon, Edison LuizMorello, Luis GustavoMarchini, Fabrício KleryntonRiediger, Irina NatassjaDebur, Maria do CarmoNakaya, Helder I.Blanes, Lucas2021-05-05T19:14:54Z2021-05-05T19:14:54Z2021AOKI, Mateus Nóbrega et al. Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load. Scientific Reports, v.11, n. 9026, p. 1–10, 2021.2045-2322https://www.arca.fiocruz.br/handle/icict/4706210.1038/s41598-021-88506-yporNatureCOVID-19RT-LAMPLoop Mediated Isothermal AmplifcationReverse TranscriptaseCoronavirus InfectionsReagent Kits, DiagnosticPoint-of-Care TestingADN Polimerasa Dirigida por ARNInfecciones por CoronavirusJuego de Reactivos para DiagnósticoPruebas en el Punto de AtenciónRNA-directed DNA polymeraseInfections à coronavirusTrousses de réactifs pour diagnosticAnalyse sur le lieu d'interventionTranscriptase ReversaInfecções por CoronavirusKit de Reagentes para DiagnósticoTestes ImediatosColorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral loadinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Universidade de São Paulo. Plataforma Científica Pasteur. São Paulo, SP, Brasil. / Universidade de São Paulo. Departamento de Microbiologia. São Paulo, SP, Brasil.Universidade de São Paulo. Plataforma Científica Pasteur. São Paulo, SP, Brasil.Universidade de São Paulo. Plataforma Científica Pasteur. São Paulo, SP, Brasil. / Universidade de São Paulo. Departamento de Microbiologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.Secretaria Estadual de Saúde. Laboratório Central do Estado do Paraná. Curitiba, PR, Brasil.Secretaria Estadual de Saúde. Laboratório Central do Estado do Paraná. Curitiba, PR, Brasil.Universidade de São Paulo. Plataforma Científica Pasteur. São Paulo, SP, Brasil. / Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Departamento de Análises Clínicas e Toxicológicas. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.The use of RT-LAMP (reverse transcriptase—loop mediated isothermal amplifcation) has been considered as a promising point-of-care method to diagnose COVID-19. In this manuscript we show that the RT-LAMP reaction has a sensitivity of only 200 RNA virus copies, with a color change from pink to yellow occurring in 100% of the 62 clinical samples tested positive by RT-qPCR. We also demonstrated that this reaction is 100% specifc for SARS-CoV-2 after testing 57 clinical samples infected with dozens of diferent respiratory viruses and 74 individuals without any viral infection. Although the majority of manuscripts recently published using this technique describe only the presence of two-color states (pink =negative and yellow=positive), we verifed by naked-eye and absorbance measurements that there is an evident third color cluster (orange), in general related to positive samples with low viral loads, but which cannot be defned as positive or negative by the naked eye. Orange colors should be repeated or tested by RT-qPCR to avoid a false diagnostic. RT-LAMP is therefore very reliable for samples with a RT-qPCR Ct < 30 being as sensitive and specifc as a RT-qPCR test. All reactions were performed in 30 min at 65 °C. The use of reaction time longer than 30 min is also not recommended since nonspecifc amplifcations may cause false positives.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/47062/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL41598_2021_Article_88506OK.pdf41598_2021_Article_88506OK.pdfapplication/pdf1560040https://www.arca.fiocruz.br/bitstream/icict/47062/2/41598_2021_Article_88506OK.pdf8417963499a4582d16632436128101b2MD52TEXT41598_2021_Article_88506OK.pdf.txt41598_2021_Article_88506OK.pdf.txtExtracted texttext/plain41455https://www.arca.fiocruz.br/bitstream/icict/47062/3/41598_2021_Article_88506OK.pdf.txt2617181787797560330af3b0f8f615caMD53icict/470622021-10-05 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dc.title.pt_BR.fl_str_mv Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
title Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
spellingShingle Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
Aoki, Mateus Nóbrega
COVID-19
RT-LAMP
Loop Mediated Isothermal Amplifcation
Reverse Transcriptase
Coronavirus Infections
Reagent Kits, Diagnostic
Point-of-Care Testing
ADN Polimerasa Dirigida por ARN
Infecciones por Coronavirus
Juego de Reactivos para Diagnóstico
Pruebas en el Punto de Atención
RNA-directed DNA polymerase
Infections à coronavirus
Trousses de réactifs pour diagnostic
Analyse sur le lieu d'intervention
Transcriptase Reversa
Infecções por Coronavirus
Kit de Reagentes para Diagnóstico
Testes Imediatos
title_short Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
title_full Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
title_fullStr Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
title_full_unstemmed Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
title_sort Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load
author Aoki, Mateus Nóbrega
author_facet Aoki, Mateus Nóbrega
Coelho, Bruna de Oliveira
Góes, Luiz Gustavo Bentim
Minoprio, Paola
Durigon, Edison Luiz
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Riediger, Irina Natassja
Debur, Maria do Carmo
Nakaya, Helder I.
Blanes, Lucas
author_role author
author2 Coelho, Bruna de Oliveira
Góes, Luiz Gustavo Bentim
Minoprio, Paola
Durigon, Edison Luiz
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Riediger, Irina Natassja
Debur, Maria do Carmo
Nakaya, Helder I.
Blanes, Lucas
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aoki, Mateus Nóbrega
Coelho, Bruna de Oliveira
Góes, Luiz Gustavo Bentim
Minoprio, Paola
Durigon, Edison Luiz
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Riediger, Irina Natassja
Debur, Maria do Carmo
Nakaya, Helder I.
Blanes, Lucas
dc.subject.other.pt_BR.fl_str_mv COVID-19
topic COVID-19
RT-LAMP
Loop Mediated Isothermal Amplifcation
Reverse Transcriptase
Coronavirus Infections
Reagent Kits, Diagnostic
Point-of-Care Testing
ADN Polimerasa Dirigida por ARN
Infecciones por Coronavirus
Juego de Reactivos para Diagnóstico
Pruebas en el Punto de Atención
RNA-directed DNA polymerase
Infections à coronavirus
Trousses de réactifs pour diagnostic
Analyse sur le lieu d'intervention
Transcriptase Reversa
Infecções por Coronavirus
Kit de Reagentes para Diagnóstico
Testes Imediatos
dc.subject.en.pt_BR.fl_str_mv RT-LAMP
Loop Mediated Isothermal Amplifcation
Reverse Transcriptase
Coronavirus Infections
Reagent Kits, Diagnostic
Point-of-Care Testing
dc.subject.es.pt_BR.fl_str_mv ADN Polimerasa Dirigida por ARN
Infecciones por Coronavirus
Juego de Reactivos para Diagnóstico
Pruebas en el Punto de Atención
dc.subject.fr.pt_BR.fl_str_mv RNA-directed DNA polymerase
Infections à coronavirus
Trousses de réactifs pour diagnostic
Analyse sur le lieu d'intervention
dc.subject.decs.pt_BR.fl_str_mv Transcriptase Reversa
Infecções por Coronavirus
Kit de Reagentes para Diagnóstico
Testes Imediatos
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-05-05T19:14:54Z
dc.date.available.fl_str_mv 2021-05-05T19:14:54Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv AOKI, Mateus Nóbrega et al. Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load. Scientific Reports, v.11, n. 9026, p. 1–10, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47062
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-021-88506-y
identifier_str_mv AOKI, Mateus Nóbrega et al. Colorimetric RT‑LAMP SARS‑CoV‑2 diagnostic sensitivity relies on color interpretation and viral load. Scientific Reports, v.11, n. 9026, p. 1–10, 2021.
2045-2322
10.1038/s41598-021-88506-y
url https://www.arca.fiocruz.br/handle/icict/47062
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