Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection

Detalhes bibliográficos
Autor(a) principal: Alves, Clarice Carvalho
Data de Publicação: 2015
Outros Autores: Araujo, Neusa, Santos, Viviane Cristina Fernandes dos, Couto, Flávia Bubula, Assis, Natan R. G., Morais, Suellen B., Oliveira, Sérgio Costa, Fonseca, Cristina Toscano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12630
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
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spelling Alves, Clarice CarvalhoAraujo, NeusaSantos, Viviane Cristina Fernandes dosCouto, Flávia BubulaAssis, Natan R. G.Morais, Suellen B.Oliveira, Sérgio CostaFonseca, Cristina Toscano2016-01-27T12:01:36Z2016-01-27T12:01:36Z2015ALVES, Clarice Carvalho et al. Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection. PLoS Negl Trop Dis. 2015 Feb 27; vol. 9, n. 2, p. e0003537, 20151935-2727https://www.arca.fiocruz.br/handle/icict/1263010.1371/journal.pntd.0003537. eCollection 2015.engPublic Library of ScienceSm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciências e Tecnologia em Doenças Tropicais. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil/Instituto Nacional de Ciências e Tecnologia em Doenças Tropicais. Salvador, BA, BrasilBACKGROUND: A vaccine against schistosomiasis would have a great impact in disease elimination. Sm29 and Sm22.6 are two parasite tegument proteins which represent promising antigens to compose a vaccine. These antigens have been associated with resistance to infection and reinfection in individuals living in endemic area for the disease and induced partial protection when evaluated in immunization trials using naïve mice. METHODOLOGY/PRINCIPALS FINDINGS: In this study we evaluated rSm29 and rSm22.6 ability to induce protection in Balb/c mice that had been previously infected with S. mansoni and further treated with Praziquantel. Our results demonstrate that three doses of the vaccine containing rSm29 were necessary to elicit significant protection (26%-48%). Immunization of mice with rSm29 induced a significant production of IL-2, IFN-γ, IL-17, IL-4; significant production of specific antibodies; increased percentage of CD4+ central memory cells in comparison with infected and treated saline group and increased percentage of CD4+ effector memory cells in comparison with naïve Balb/c mice immunized with rSm29. On the other hand, although immunization with Sm22.6 induced a robust immune response, it failed to induce protection. CONCLUSION/SIGNIFICANCE: Our results demonstrate that rSm29 retains its ability to induce protection in previously infected animals, reinforcing its potential as a vaccine candidate.Antibodies, Helminth/bloodAntigens, Helminth/immunologyCD4-Positive T-Lymphocytes/immunologyHelminth Proteins/immunologyMembrane Glycoproteins/immunologySchistosoma mansoni/immunologyVaccines/immunologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/12630/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSm29, but not Sm22.6 retains its ability to induce .pdfSm29, but not Sm22.6 retains its ability to induce .pdfapplication/pdf547543https://www.arca.fiocruz.br/bitstream/icict/12630/2/Sm29%2c%20but%20not%20Sm22.6%20retains%20its%20ability%20to%20induce%20.pdfd88539029da829a8674e2fc5fca21f71MD52TEXTSm29, but not Sm22.6 retains its ability to induce .pdf.txtSm29, but not Sm22.6 retains its ability to induce .pdf.txtExtracted texttext/plain63176https://www.arca.fiocruz.br/bitstream/icict/12630/3/Sm29%2c%20but%20not%20Sm22.6%20retains%20its%20ability%20to%20induce%20.pdf.txt599ffe824d22921ae72104b75ccf03c6MD53icict/126302019-06-19 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dc.title.pt_BR.fl_str_mv Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
title Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
spellingShingle Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
Alves, Clarice Carvalho
Antibodies, Helminth/blood
Antigens, Helminth/immunology
CD4-Positive T-Lymphocytes/immunology
Helminth Proteins/immunology
Membrane Glycoproteins/immunology
Schistosoma mansoni/immunology
Vaccines/immunology
title_short Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
title_full Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
title_fullStr Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
title_full_unstemmed Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
title_sort Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection
author Alves, Clarice Carvalho
author_facet Alves, Clarice Carvalho
Araujo, Neusa
Santos, Viviane Cristina Fernandes dos
Couto, Flávia Bubula
Assis, Natan R. G.
Morais, Suellen B.
Oliveira, Sérgio Costa
Fonseca, Cristina Toscano
author_role author
author2 Araujo, Neusa
Santos, Viviane Cristina Fernandes dos
Couto, Flávia Bubula
Assis, Natan R. G.
Morais, Suellen B.
Oliveira, Sérgio Costa
Fonseca, Cristina Toscano
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Alves, Clarice Carvalho
Araujo, Neusa
Santos, Viviane Cristina Fernandes dos
Couto, Flávia Bubula
Assis, Natan R. G.
Morais, Suellen B.
Oliveira, Sérgio Costa
Fonseca, Cristina Toscano
dc.subject.en.pt_BR.fl_str_mv Antibodies, Helminth/blood
Antigens, Helminth/immunology
CD4-Positive T-Lymphocytes/immunology
Helminth Proteins/immunology
Membrane Glycoproteins/immunology
Schistosoma mansoni/immunology
Vaccines/immunology
topic Antibodies, Helminth/blood
Antigens, Helminth/immunology
CD4-Positive T-Lymphocytes/immunology
Helminth Proteins/immunology
Membrane Glycoproteins/immunology
Schistosoma mansoni/immunology
Vaccines/immunology
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-01-27T12:01:36Z
dc.date.available.fl_str_mv 2016-01-27T12:01:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVES, Clarice Carvalho et al. Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection. PLoS Negl Trop Dis. 2015 Feb 27; vol. 9, n. 2, p. e0003537, 2015
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12630
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0003537. eCollection 2015.
identifier_str_mv ALVES, Clarice Carvalho et al. Sm29, but not Sm22.6 retains its ability to induce a protective immune response in mice previously exposed to a Schistosoma mansoni infection. PLoS Negl Trop Dis. 2015 Feb 27; vol. 9, n. 2, p. e0003537, 2015
1935-2727
10.1371/journal.pntd.0003537. eCollection 2015.
url https://www.arca.fiocruz.br/handle/icict/12630
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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