CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Santos, Claire da Silva
Data de Publicação: 2013
Outros Autores: Boaventura, Viviane, Cardoso, Cristina Ribeiro, Tavares, Natalia, Lordelo, Morgana de Jesus, Noronha, Almério, Costa, Jackson, Borges, Valéria de Matos, Oliveira, Camila Indiani de, Van Weyenbergh, Johan, Barral, Aldina Maria Prado, Barral Netto, Manoel, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20743
Resumo: Fundação de Amparo a Pesquisa do Estado da Bahia (FAPESB) e/Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq grant number PPSUS # SUS0025/2009) and from Pronex (FAPESB/CNPq grant number 738712006). CdaSS has a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.
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spelling Santos, Claire da SilvaBoaventura, VivianeCardoso, Cristina RibeiroTavares, NataliaLordelo, Morgana de JesusNoronha, AlmérioCosta, JacksonBorges, Valéria de MatosOliveira, Camila Indiani deVan Weyenbergh, JohanBarral, Aldina Maria PradoBarral Netto, ManoelBrodskyn, Claudia Ida2017-08-24T16:25:38Z2017-08-24T16:25:38Z2013SANTOS, C. S. et al. CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis. Journal of Investigative Dermatology, v. 133, p. 1533–1540, 2013.0022-202Xhttps://www.arca.fiocruz.br/handle/icict/2074310.1038/jid.2013.4Fundação de Amparo a Pesquisa do Estado da Bahia (FAPESB) e/Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq grant number PPSUS # SUS0025/2009) and from Pronex (FAPESB/CNPq grant number 738712006). CdaSS has a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade de São Paulo. Faculdade de Ciências Farmacêuticas de São Paulo. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. São Paulo, SP, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrazilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrazilUniversidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brazil /A protective or deleterious role of CD8(+)T cells in human cutaneous leishmaniasis (CL) has been debated. The present report explores the participation of CD8(+)T cells in disease pathogenesis as well as in parasite killing. CD8(+)T cells accumulated in CL lesions as suggested by a higher frequency of CD8(+)CD45RO(+)T cells and CD8(+)CLA(+)T cells compared with peripheral blood mononuclear cells. Upon Leishmania braziliensis restimulation, most of the CD8(+)T cells from the lesion expressed cytolytic markers, CD107a and granzyme B. Granzyme B expression in CL lesions positively correlated with lesion size and percentage of TUNEL-positive cells. We also observed a significantly higher percentage of TUNEL-positive cells and granzyme B expression in the biopsies of patients showing a more intense necrotic process. Furthermore, coculture of infected macrophages and CD8(+)T lymphocytes resulted in the release of granzyme B, and the use of granzyme B inhibitor, as well as z-VAD, Fas:Fc, or anti-IFN-γ, had no effect upon parasite killing. However, coculture of infected macrophages with CD4(+)T cells strongly increased parasite killing, which was completely reversed by anti-IFN-γ. Our results reveal a dichotomy in human CL: CD8(+) granzyme B(+)T cells mediate tissue injury, whereas CD4(+)IFN-γ(+)T cells mediate parasite killing.engElsevierLeishmania braziliensisLeishmaniose CutâneaBiomarcadoresLinfócitos T CD8 positivosLinfócitos T CD4 positivosHumanosAdultoAdolescenteLeishmania braziliensisLeishmaniasis, CutaneousBiomarkersCD8-Positive T-LymphocytesCD4-Positive T-LymphocytesHumansAdultAdolescentCD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20743/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSilva C CD8+Granzyme B+ T cells-mediated... .pdfSilva C CD8+Granzyme B+ T 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dc.title.pt_BR.fl_str_mv CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
title CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
spellingShingle CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
Santos, Claire da Silva
Leishmania braziliensis
Leishmaniose Cutânea
Biomarcadores
Linfócitos T CD8 positivos
Linfócitos T CD4 positivos
Humanos
Adulto
Adolescente
Leishmania braziliensis
Leishmaniasis, Cutaneous
Biomarkers
CD8-Positive T-Lymphocytes
CD4-Positive T-Lymphocytes
Humans
Adult
Adolescent
title_short CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
title_full CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
title_fullStr CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
title_full_unstemmed CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
title_sort CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis
author Santos, Claire da Silva
author_facet Santos, Claire da Silva
Boaventura, Viviane
Cardoso, Cristina Ribeiro
Tavares, Natalia
Lordelo, Morgana de Jesus
Noronha, Almério
Costa, Jackson
Borges, Valéria de Matos
Oliveira, Camila Indiani de
Van Weyenbergh, Johan
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
author_role author
author2 Boaventura, Viviane
Cardoso, Cristina Ribeiro
Tavares, Natalia
Lordelo, Morgana de Jesus
Noronha, Almério
Costa, Jackson
Borges, Valéria de Matos
Oliveira, Camila Indiani de
Van Weyenbergh, Johan
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Claire da Silva
Boaventura, Viviane
Cardoso, Cristina Ribeiro
Tavares, Natalia
Lordelo, Morgana de Jesus
Noronha, Almério
Costa, Jackson
Borges, Valéria de Matos
Oliveira, Camila Indiani de
Van Weyenbergh, Johan
Barral, Aldina Maria Prado
Barral Netto, Manoel
Brodskyn, Claudia Ida
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniose Cutânea
Biomarcadores
Linfócitos T CD8 positivos
Linfócitos T CD4 positivos
Humanos
Adulto
Adolescente
topic Leishmania braziliensis
Leishmaniose Cutânea
Biomarcadores
Linfócitos T CD8 positivos
Linfócitos T CD4 positivos
Humanos
Adulto
Adolescente
Leishmania braziliensis
Leishmaniasis, Cutaneous
Biomarkers
CD8-Positive T-Lymphocytes
CD4-Positive T-Lymphocytes
Humans
Adult
Adolescent
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Leishmaniasis, Cutaneous
Biomarkers
CD8-Positive T-Lymphocytes
CD4-Positive T-Lymphocytes
Humans
Adult
Adolescent
description Fundação de Amparo a Pesquisa do Estado da Bahia (FAPESB) e/Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq grant number PPSUS # SUS0025/2009) and from Pronex (FAPESB/CNPq grant number 738712006). CdaSS has a fellowship from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Brazil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2017-08-24T16:25:38Z
dc.date.available.fl_str_mv 2017-08-24T16:25:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SANTOS, C. S. et al. CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis. Journal of Investigative Dermatology, v. 133, p. 1533–1540, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20743
dc.identifier.issn.pt_BR.fl_str_mv 0022-202X
dc.identifier.doi.none.fl_str_mv 10.1038/jid.2013.4
identifier_str_mv SANTOS, C. S. et al. CD8(+) granzyme B(+)-mediated tissue injury vs. CD4(+)IFNγ(+)-mediated parasite killing in human cutaneous leishmaniasis. Journal of Investigative Dermatology, v. 133, p. 1533–1540, 2013.
0022-202X
10.1038/jid.2013.4
url https://www.arca.fiocruz.br/handle/icict/20743
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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