CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13576 |
Resumo: | Restrito: p. 1533-1540 |
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Santos, Claire da SilvaBoaventura, Viviane SampaioCardoso, Cristina RibeiroTavares, Natália MachadoLordelo, Morgana de JesusNoronha, Almério Libório Lopes deNoronha, Almério Libório Lopes deCosta, JacksonBorges, Valéria de MatosOliveira, Camila Indiani deWeyenbergh, Johan VanBarral, Aldina Maria PradoBarral-Netto, ManoelBrodskyn, Claudia IdaSantos, Claire da SilvaBoaventura, Viviane SampaioCardoso, Cristina RibeiroTavares, Natália MachadoLordelo, Morgana de JesusNoronha, Almério Libório Lopes deNoronha, Almério Libório Lopes deCosta, JacksonBorges, Valéria de MatosOliveira, Camila Indiani deWeyenbergh, Johan VanBarral, Aldina Maria PradoBarral-Netto, ManoelBrodskyn, Claudia Ida2013-11-06T20:59:24Z20130022-202Xhttp://repositorio.ufba.br/ri/handle/ri/13576Restrito: p. 1533-1540A protective or deleterious role of CD8þT cells in human cutaneous leishmaniasis (CL) has been debated. The present report explores the participation of CD8þT cells in disease pathogenesis as well as in parasite killing. CD8þT cells accumulated in CL lesions as suggested by a higher frequency of CD8þCD45ROþT cells and CD8þCLAþT cells compared with peripheral blood mononuclear cells. Upon Leishmania braziliensis restimulation, most of the CD8þT cells from the lesion expressed cytolytic markers, CD107a and granzyme B. Granzyme B expression in CL lesions positively correlated with lesion size and percentage of TUNEL-positive cells. We also observed a significantly higher percentage of TUNEL-positive cells and granzyme B expression in the biopsies of patients showing a more intense necrotic process. Furthermore, coculture of infected macrophages and CD8þ T lymphocytes resulted in the release of granzyme B, and the use of granzyme B inhibitor, as well as z-VAD, Fas:Fc, or anti-IFN-g, had no effect upon parasite killing. However, coculture of infected macrophages with CD4þT cells strongly increased parasite killing, which was completely reversed by anti-IFN-g. Our results reveal a dichotomy in human CL: CD8þ granzyme BþT cells mediate tissue injury, whereas CD4þIFN-gþT cells mediate parasite killing.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-11-05T20:09:33Z No. of bitstreams: 1 5555555.pdf: 4914876 bytes, checksum: e54c8737a502be3105cd578fa7d10ef4 (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2013-11-06T20:59:23Z (GMT) No. of bitstreams: 1 5555555.pdf: 4914876 bytes, checksum: e54c8737a502be3105cd578fa7d10ef4 (MD5)Made available in DSpace on 2013-11-06T20:59:24Z (GMT). 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| dc.title.pt_BR.fl_str_mv |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Investigative Dermatology |
| title |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| spellingShingle |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis Santos, Claire da Silva Leishmania braziliensis CD8 þ T cells Granzyme B Macrophages |
| title_short |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| title_full |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| title_fullStr |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| title_full_unstemmed |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| title_sort |
CD8þ Granzyme Bþ–Mediated Tissue Injury vs. CD4þIFNcþ–Mediated Parasite Killing in Human Cutaneous Leishmaniasis |
| author |
Santos, Claire da Silva |
| author_facet |
Santos, Claire da Silva Boaventura, Viviane Sampaio Cardoso, Cristina Ribeiro Tavares, Natália Machado Lordelo, Morgana de Jesus Noronha, Almério Libório Lopes de Costa, Jackson Borges, Valéria de Matos Oliveira, Camila Indiani de Weyenbergh, Johan Van Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Boaventura, Viviane Sampaio Cardoso, Cristina Ribeiro Tavares, Natália Machado Lordelo, Morgana de Jesus Noronha, Almério Libório Lopes de Costa, Jackson Borges, Valéria de Matos Oliveira, Camila Indiani de Weyenbergh, Johan Van Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Claire da Silva Boaventura, Viviane Sampaio Cardoso, Cristina Ribeiro Tavares, Natália Machado Lordelo, Morgana de Jesus Noronha, Almério Libório Lopes de Noronha, Almério Libório Lopes de Costa, Jackson Borges, Valéria de Matos Oliveira, Camila Indiani de Weyenbergh, Johan Van Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida Santos, Claire da Silva Boaventura, Viviane Sampaio Cardoso, Cristina Ribeiro Tavares, Natália Machado Lordelo, Morgana de Jesus Noronha, Almério Libório Lopes de Noronha, Almério Libório Lopes de Costa, Jackson Borges, Valéria de Matos Oliveira, Camila Indiani de Weyenbergh, Johan Van Barral, Aldina Maria Prado Barral-Netto, Manoel Brodskyn, Claudia Ida |
| dc.subject.por.fl_str_mv |
Leishmania braziliensis CD8 þ T cells Granzyme B Macrophages |
| topic |
Leishmania braziliensis CD8 þ T cells Granzyme B Macrophages |
| description |
Restrito: p. 1533-1540 |
| publishDate |
2013 |
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2013-11-06T20:59:24Z |
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2013 |
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0022-202X |
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0022-202X |
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Journal of Investigative Dermatology |
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Journal of Investigative Dermatology |
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http://www.nature.com/jid/journal/v133/n6/pdf/jid20134a.pdf |
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