Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation

Detalhes bibliográficos
Autor(a) principal: Cesaro, Giovanna
Data de Publicação: 2019
Outros Autores: Carneiro, Flávia Raquel Gonçalves, Ávila, Andréa Rodrigues, Zanchin, Nilson Ivo Tonin, Guimarães, Beatriz Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59632
Resumo: Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil / Federal University of Parana. Biochemsitry Postgraduate Program. Curitiba, PR, Brazil.
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spelling Cesaro, GiovannaCarneiro, Flávia Raquel GonçalvesÁvila, Andréa RodriguesZanchin, Nilson Ivo ToninGuimarães, Beatriz Gomes2023-07-18T04:44:27Z2023-07-18T04:44:27Z2019CESARO, Giovanna et al. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biology, v. 16, n. 1, p. 133-143, Jan. 2019.1547-6286https://www.arca.fiocruz.br/handle/icict/5963210.1080/15476286.2018.15644631555-8584engTaylor and Francis Inc.https://www.arca.fiocruz.br/handle/icict/59874AnimalsCattleCells CulturedExosome Multienzyme Ribonuclease ComplexHost-Parasite InteractionsModels, MolecularProtein BindingProtein ConformationProtozoan ProteinsRNA Processing, Post-TranscriptionalRNA, RibosomalStructure-Activity RelationshipTrypanosoma brucei bruceiTrypanosomiasis, BovinerRNA processingTrypanosoma bruceiRibonucleaseRRP44PIN domain 3D structureTrypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil / Federal University of Parana. Biochemsitry Postgraduate Program. Curitiba, PR, Brazil.Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil / Oswaldo Cruz Foundation. Center for Technology Development in Healthcare. Rio de Janeiro, RJ, Brazil.Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil.Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil.Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil.Ribosomal RNA precursors undergo a series of structural and chemical modifications to generate matured RNA molecules that will comprise ribosomes. This maturation process involves a large set of accessory proteins as well as ribonucleases, responsible for removal of the external and internal transcribed spacers from the pre-rRNA. Early-diverging eukaryotes belonging to the Kinetoplastida class display several unique characteristics, in particular in terms of RNA synthesis and maturation. These peculiarities include the rRNA biogenesis and the extensive fragmentation of the large ribosomal subunit (LSU) rRNA. The role of specific endo- and exonucleases in the maturation of the unusual rRNA precursor of trypanosomatids remains largely unknown. One of the nucleases involved in rRNA processing is Rrp44, an exosome associated ribonuclease in yeast, which is involved in several metabolic RNA pathways. Here, we investigated the function of Trypanosoma brucei RRP44 orthologue (TbRRP44) in rRNA processing. Our results revealed that TbRRP44 depletion causes unusual polysome profile and accumulation of the complete LSU rRNA precursor, in addition to 5.8S maturation impairment. We also determined the crystal structure of TbRRP44 endonucleolytic domain. Structural comparison with Saccharomyces cerevisiae Rrp44 revealed differences in the catalytic site and substitutions of surface residues, which could provide molecular bases for the lack of interaction of RRP44 with the exosome complex in T. brucei.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59632/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.1080_15476286.2018.1564463.pdfapplication/pdf6808691https://www.arca.fiocruz.br/bitstream/icict/59632/2/10.1080_15476286.2018.1564463.pdfbc741bdc36c7c5a4bafc9ab2253b9515MD52icict/596322023-08-02 09:52:44.364oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-08-02T12:52:44Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
spellingShingle Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
Cesaro, Giovanna
Animals
Cattle
Cells Cultured
Exosome Multienzyme Ribonuclease Complex
Host-Parasite Interactions
Models, Molecular
Protein Binding
Protein Conformation
Protozoan Proteins
RNA Processing, Post-Transcriptional
RNA, Ribosomal
Structure-Activity Relationship
Trypanosoma brucei brucei
Trypanosomiasis, Bovine
rRNA processing
Trypanosoma brucei
Ribonuclease
RRP44
PIN domain 3D structure
title_short Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_full Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_fullStr Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_full_unstemmed Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_sort Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
author Cesaro, Giovanna
author_facet Cesaro, Giovanna
Carneiro, Flávia Raquel Gonçalves
Ávila, Andréa Rodrigues
Zanchin, Nilson Ivo Tonin
Guimarães, Beatriz Gomes
author_role author
author2 Carneiro, Flávia Raquel Gonçalves
Ávila, Andréa Rodrigues
Zanchin, Nilson Ivo Tonin
Guimarães, Beatriz Gomes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cesaro, Giovanna
Carneiro, Flávia Raquel Gonçalves
Ávila, Andréa Rodrigues
Zanchin, Nilson Ivo Tonin
Guimarães, Beatriz Gomes
dc.subject.mesh.en_US.fl_str_mv Animals
Cattle
Cells Cultured
Exosome Multienzyme Ribonuclease Complex
Host-Parasite Interactions
Models, Molecular
Protein Binding
Protein Conformation
Protozoan Proteins
RNA Processing, Post-Transcriptional
RNA, Ribosomal
Structure-Activity Relationship
Trypanosoma brucei brucei
Trypanosomiasis, Bovine
topic Animals
Cattle
Cells Cultured
Exosome Multienzyme Ribonuclease Complex
Host-Parasite Interactions
Models, Molecular
Protein Binding
Protein Conformation
Protozoan Proteins
RNA Processing, Post-Transcriptional
RNA, Ribosomal
Structure-Activity Relationship
Trypanosoma brucei brucei
Trypanosomiasis, Bovine
rRNA processing
Trypanosoma brucei
Ribonuclease
RRP44
PIN domain 3D structure
dc.subject.en.en_US.fl_str_mv rRNA processing
Trypanosoma brucei
Ribonuclease
RRP44
PIN domain 3D structure
description Oswaldo Cruz Foundation. Carlos Chagas Institute. Curitiba, PR, Brazil / Federal University of Parana. Biochemsitry Postgraduate Program. Curitiba, PR, Brazil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2023-07-18T04:44:27Z
dc.date.available.fl_str_mv 2023-07-18T04:44:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CESARO, Giovanna et al. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biology, v. 16, n. 1, p. 133-143, Jan. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59632
dc.identifier.issn.en_US.fl_str_mv 1547-6286
dc.identifier.doi.none.fl_str_mv 10.1080/15476286.2018.1564463
dc.identifier.eissn.none.fl_str_mv 1555-8584
identifier_str_mv CESARO, Giovanna et al. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biology, v. 16, n. 1, p. 133-143, Jan. 2019.
1547-6286
10.1080/15476286.2018.1564463
1555-8584
url https://www.arca.fiocruz.br/handle/icict/59632
dc.language.iso.fl_str_mv eng
language eng
dc.relation.hasversion.none.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59874
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor and Francis Inc.
publisher.none.fl_str_mv Taylor and Francis Inc.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/59632/2/10.1080_15476286.2018.1564463.pdf
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