Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation

Detalhes bibliográficos
Autor(a) principal: Cesaro, Giovanna
Data de Publicação: 2019
Outros Autores: Carneiro, Flávia Raquel Gonçalves, Ávila, Andréa Rodrigues, Zanchin, Nilson Ivo Tonin, Guimarães, Beatriz Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1080/15476286.2018.1564463
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59874
Resumo: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES Financing code 001 and CAPES-COFECUB grant 862-15), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, NITZ 312195/2015-0 and GC fellow-ship) and Fundação Oswaldo Cruz.
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spelling Cesaro, GiovannaCarneiro, Flávia Raquel GonçalvesÁvila, Andréa RodriguesZanchin, Nilson Ivo ToninGuimarães, Beatriz Gomes2023-08-02T12:48:48Z2023-08-02T12:48:48Z2019CESARO, Giovanna et al. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biology, v. 16, n. 1, p. 133-143, 7 Jan. 2019.1547-6286https://www.arca.fiocruz.br/handle/icict/5987410.1080/15476286.2018.15644631555-8584Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES Financing code 001 and CAPES-COFECUB grant 862-15), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, NITZ 312195/2015-0 and GC fellow-ship) and Fundação Oswaldo Cruz.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências – Bioquímica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Ribosomal RNA precursors undergo a series of structural and chemical modifications to generate matured RNA molecules that will comprise ribosomes. This maturation process involves a large set of accessory proteins as well as ribonucleases, responsible for removal of the external and internal transcribed spacers from the pre-rRNA. Early-diverging eukaryotes belonging to the Kinetoplastida class display several unique characteristics, in particular in terms of RNA synthesis and maturation. These peculiarities include the rRNA biogenesis and the extensive fragmentation of the large ribosomal subunit (LSU) rRNA. The role of specific endo- and exonucleases in the maturation of the unusual rRNA precursor of trypanosomatids remains largely unknown. One of the nucleases involved in rRNA processing is Rrp44, an exosome associated ribonuclease in yeast, which is involved in several metabolic RNA pathways. Here, we investigated the function of Trypanosoma brucei RRP44 orthologue (TbRRP44) in rRNA processing. Our results revealed that TbRRP44 depletion causes unusual polysome profile and accumulation of the complete LSU rRNA precursor, in addition to 5.8S maturation impairment. We also determined the crystal structure of TbRRP44 endonucleolytic domain. Structural comparison with Saccharomyces cerevisiae Rrp44 revealed differences in the catalytic site and substitutions of surface residues, which could provide molecular bases for the lack of interaction of RRP44 with the exosome complex in T. brucei.engTaylor and Francis Grouphttps://www.arca.fiocruz.br/handle/icict/59632Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlerRNA processingTrypanosoma bruceiribonucleaseRRP44PIN domain 3D structureinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.en_US.fl_str_mv Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
spellingShingle Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
Cesaro, Giovanna
rRNA processing
Trypanosoma brucei
ribonuclease
RRP44
PIN domain 3D structure
title_short Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_full Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_fullStr Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_full_unstemmed Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
title_sort Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation
author Cesaro, Giovanna
author_facet Cesaro, Giovanna
Carneiro, Flávia Raquel Gonçalves
Ávila, Andréa Rodrigues
Zanchin, Nilson Ivo Tonin
Guimarães, Beatriz Gomes
author_role author
author2 Carneiro, Flávia Raquel Gonçalves
Ávila, Andréa Rodrigues
Zanchin, Nilson Ivo Tonin
Guimarães, Beatriz Gomes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cesaro, Giovanna
Carneiro, Flávia Raquel Gonçalves
Ávila, Andréa Rodrigues
Zanchin, Nilson Ivo Tonin
Guimarães, Beatriz Gomes
dc.subject.en.en_US.fl_str_mv rRNA processing
Trypanosoma brucei
ribonuclease
RRP44
PIN domain 3D structure
topic rRNA processing
Trypanosoma brucei
ribonuclease
RRP44
PIN domain 3D structure
description Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES Financing code 001 and CAPES-COFECUB grant 862-15), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, NITZ 312195/2015-0 and GC fellow-ship) and Fundação Oswaldo Cruz.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2023-08-02T12:48:48Z
dc.date.available.fl_str_mv 2023-08-02T12:48:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CESARO, Giovanna et al. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biology, v. 16, n. 1, p. 133-143, 7 Jan. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59874
dc.identifier.issn.en_US.fl_str_mv 1547-6286
dc.identifier.doi.none.fl_str_mv 10.1080/15476286.2018.1564463
dc.identifier.eissn.none.fl_str_mv 1555-8584
identifier_str_mv CESARO, Giovanna et al. Trypanosoma brucei RRP44 is involved in an early stage of large ribosomal subunit RNA maturation. RNA Biology, v. 16, n. 1, p. 133-143, 7 Jan. 2019.
1547-6286
10.1080/15476286.2018.1564463
1555-8584
url https://www.arca.fiocruz.br/handle/icict/59874
dc.language.iso.fl_str_mv eng
language eng
dc.relation.isversionof.en_US.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59632
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Taylor and Francis Group
publisher.none.fl_str_mv Taylor and Francis Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59874/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/59874/2/ve_Giovanna_Cesaro_etal_CDTS_2019_ARTIGO_FINAL.pdf
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